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30/04/2015 Enzymes used in the detergent industry.

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Presentation on theme: "30/04/2015 Enzymes used in the detergent industry."— Presentation transcript:

1 30/04/2015 Enzymes used in the detergent industry

2 NOVOZYMES PRESENTATION30/04/20152 4 Types of enzymes are used for different detergents Cellulase Cellulose Cotton, viscose Carbohydrases Amylase Lipase Protease Starch Triglycerides Proteins Examples Rice, noodles, potatoes, corn Organic fats and oils, sebum, lipstick Substrate Blood, egg, milk grass, meat

3 NOVOZYMES PRESENTATION30/04/20153 Catalytic actions of detergent enzymes

4 NOVOZYMES PRESENTATION30/04/20154 Stain removal  Surfactants can remove most of the soils in laundry. Why do we need the enzymes?  Enzymes and surfactants generally work complementary, and together they can do the cleaning job at low temperature + mild pH  The following cartoon shows how enzymes degrade soil partially and surfactants bring the reminder into suspension

5 NOVOZYMES PRESENTATION30/04/20155 Removal of stain 1: The stain is bound to a surface Stain Substrate Fibre

6 NOVOZYMES PRESENTATION30/04/20156 Removal of stain 2: A surfactant has been added Soil Fibre Surfactant

7 NOVOZYMES PRESENTATION30/04/20157 Removal of stain 3: Enzymes are added and degrade available sites Soil Fibre Surfactant Enzyme

8 NOVOZYMES PRESENTATION30/04/20158 Removal of Stain 4: The soil comes loose and goes into suspension Soil Fibre Surfactant Enzyme

9 NOVOZYMES PRESENTATION30/04/20159 General principle of stain removal Stain Clean vest Stain less visible Stain no longer visible Wash, no enzýmes Wash with enzymes 

10 NOVOZYMES PRESENTATION30/04/201510 Removal of invisible stains: redeposition Conclusion: Enzymes give more permanent cleaning! Washed in dirty water, No enzymes Stain Washed Clean vest Washed in dirty water, With enzymes 

11 NOVOZYMES PRESENTATION30/04/201511 Removal of inorganic particulate soils H2: washed with cleaning cellulase H1: washed without cellulase

12 NOVOZYMES PRESENTATION30/04/201512 Removal of fuzz and pills With the care cellulase: Carezyme ® Without cellulase

13 NOVOZYMES PRESENTATION30/04/201513 Enzymes boost the removal of … Food and plant stains in laundry and ADW Invisible impurities in laundry and ADW Particulate inorganic soils in laundry Fuzz and pills in laundry BUT….. Which enzymes for what?

14 NOVOZYMES PRESENTATION30/04/201514 International brand support model Basic performance Lifestyle Aesthetics Protease Amylase Lipase Cellulase

15 NOVOZYMES PRESENTATION30/04/201515 International brand support model Polarzyme Stainzyme

16 NOVOZYMES PRESENTATION30/04/201516 Proteases

17 NOVOZYMES PRESENTATION30/04/201517 Proteases from Novozymes Alcalase ® For low pH, soft on wool Esperase ® For high pH, mainly I&I* Everlase ® Relatively stable in presence of bleach Savinase ® High performance standard protease Polarzyme ® High performance low temp. protease Ovozyme ® Tolerates protease inhibitors from eggs Relase ® For liquid detergents Coronase ® For liquid detergents *I&I = Industrial and Institutional cleaning

18 NOVOZYMES PRESENTATION30/04/201518 Proteases catalyze the hydrolytic cleavage of the peptide chain Protease -R 1 CH-CO-NH-CR 2 H- + H 2 O → -CR 1 H-COO - + + H 3 N-CR 2 H- Proteases can provide: stain removal cleanliness general whitening

19 NOVOZYMES PRESENTATION30/04/201519 Facts about proteases  Novozymes proteases have been used since 1963  Are used in 75 % of all detergents  Protease is a basic ingredient in laundry and ADW detergents  Protease effects cannot be substituted by any other ingredients

20 NOVOZYMES PRESENTATION30/04/201520 Protease effect on blood spots not washedwashed 3 times, no protease washed 3 times, with protease

21 NOVOZYMES PRESENTATION30/04/201521 Stain removal with protease in laundry Protease no yes Cocoa (CFT) Blood (CFT) Salmon paste (Equest) Grass (Equest) Spinach (Equest) Olives (Equest)

22 NOVOZYMES PRESENTATION30/04/201522 Protease effect on blood spots Protease effect after 3 wash cycles and re-soiling No protease With Protease Re-soiling

23 NOVOZYMES PRESENTATION30/04/201523 Proteases perform well at high pH Relative Activity (%) The figure shows Alcalase versus Savinase pH

24 NOVOZYMES PRESENTATION30/04/201524 Wash performance of polarzyme 12T Unwashed 1) TOM (Tergotometer) is a small scale method simulating top loading US washing machine 25 °C, TOM1, Regular powder, 10 °dH, 20 min, EMPA 117 swatch

25 NOVOZYMES PRESENTATION30/04/201525 Protease sensitive test swatches:  CFT C-10Pigment/Oil/Milk on cotton  CFT C-03Chocolate milk/Soot on cotton  EMPA112Cocoa on cotton  CFT C-05Blood/Milk/Ink on cotton  EMPA116Blood/Milk/Ink on cotton  EMPA117Blood/Milk/Ink on cotton/polyester  EMPA111Blood on cotton  CS-8 Grass on cotton  PCS-8 Grass on cotton/polyester  EMPA164 Grass on cotton  WfK 10NEgg on cotton  WfK 20NEgg on cotton/polyester

26 NOVOZYMES PRESENTATION30/04/201526 Low temperature profile of polarzyme Activity profile of Polarzyme shifted towards lower temperatures maintaining activity at high temperature

27 NOVOZYMES PRESENTATION30/04/201527 Protease benefit tested in wash & wear test  Consumer wash and wear test: 70 men asked to wear a white cotton T-shirt  T-shirts collected and cut in half  One half washed in detergent with 0.1% Polarzyme 12T  Other half washed in well known non-biological brand  T-shirts sewed up, redistributed for another wearing and washing cycle (4 cycles in total)  After each cycle the person responsible for the family´s laundry was asked to rate the result

28 NOVOZYMES PRESENTATION30/04/201528 Protease benefit in wash & wear test - overall consumer preference

29 NOVOZYMES PRESENTATION30/04/201529 Protease benefit in wash & wear test consumer preference for each performance attribute

30 NOVOZYMES PRESENTATION30/04/201530 Status for proteases  Protease is applied in laundry and ADW for complete removal of protein  Protease is recognised world-wide as a neccesary provider of basic detergency

31 NOVOZYMES PRESENTATION30/04/201531 Lipases

32 NOVOZYMES PRESENTATION30/04/201532 Lipase mechanism Lipase No odor Lipase Odor + + Short chain fatty acids (C 4 –C 8) Dairy soils

33 NOVOZYMES PRESENTATION30/04/201533 Lipases from Novozymes  Lipolase®Original lipase wild type lipase. Multi wash lipase  Lipex® First wash protein engineered Lipase. Stain removal and anti redeposition effects.

34 NOVOZYMES PRESENTATION30/04/201534 Effects with Lipase  First Lipase introduced in 1988  First protein engineered lipase introduced in 1998  Lipase can to some extent replace surfactants  Remove fatty matter at low temperature  Provide stain removal  Give in-depth cleaning  Reduces redeposition  General whitening + Lipase - Lipase Lipstick

35 NOVOZYMES PRESENTATION30/04/201535 Activity of Lipolase during drying Relative Activity (%)

36 NOVOZYMES PRESENTATION30/04/201536 Lipase performance  Lipolase works best at low water concentration i.e. during drying of laundry  Lipex also works at high water concentrations such as in the main wash

37 NOVOZYMES PRESENTATION30/04/201537 Lipase for fat removal from outside of cotton fibres no lipase with lipase Lard Sebum Olive Oil

38 NOVOZYMES PRESENTATION30/04/201538 Lipase effect on inside of cotton fibres Olive oil Unwashed Olive oil Washed with detergent Olive oil Washed with detergent & lipase Cross sectional specimen of yarns soiled with olive oil

39 NOVOZYMES PRESENTATION30/04/201539 Lipase 1’st wash effect on polyester washed without lipase washed with lipase 0.2 mm

40 NOVOZYMES PRESENTATION30/04/201540 Lipex - visual soil removal in 1st wash No LipexLipex 500 LU/L Lipex 1000LU/L

41 NOVOZYMES PRESENTATION30/04/201541 Anti-redeposition benefits after 1st wash - a secondary effect of Lipases Unwashed textiles - Lipex + Lipex

42 NOVOZYMES PRESENTATION30/04/201542 Status for Lipases  Lipase can be used in laundry for complete lipid removal especially at lower temperatures  Secondary effect of lipases are contribution to general cleanness by preventing redeposition of fats, oils and colorants  In depth cleaning of textile fibres  There is a smell issue that limits the dosage

43 NOVOZYMES PRESENTATION30/04/201543 Amylases

44 NOVOZYMES PRESENTATION30/04/201544 Amylases from Novozymes  Termamyl®Original amylase for high temp.  Termamyl® UltraHighly stable liquid amylase  Duramyl® Improved stability in the presence of bleach  Stainzyme®High performance “Stainfighter”. Performs well under low temperature wash conditions

45 NOVOZYMES PRESENTATION30/04/201545 -Amylases  Amylases hydrolyse a-1,4-glucose-linkages randomly forming soluble dextrins and oligosaccharides  The viscosity of the gelatinized starch is reduced and the solubility enhanced

46 NOVOZYMES PRESENTATION30/04/201546 Removal of soils containing starch  Gelatinization and swelling are needed to make the starch susceptible to enzymatic breakdown  For most starchy foods, various degrees of gelatinization result from cooking  Amylases boost the removal of starch e.g. from pasta, potato, gravy, chocolate, and baby food  Amylases prevent starch from adhering to the surface of laundry and dishes and act as a glue for particulate soils

47 NOVOZYMES PRESENTATION30/04/201547 Removal of invisible starch stain Washed in dirty water, No enzymes Starch stain Washed in any detergent Clean vest Washed in dirty water, With enzymes

48 NOVOZYMES PRESENTATION30/04/201548 pH-profile of Termamyl and Stainzyme

49 NOVOZYMES PRESENTATION30/04/201549 Temp. profile for Termamyl and Stainzyme

50 NOVOZYMES PRESENTATION30/04/201550 Washing time

51 NOVOZYMES PRESENTATION30/04/201551 Different pH profiles

52 NOVOZYMES PRESENTATION30/04/201552 Potato starch Cotton CFT CS-27 Polyester CFT PS-27 Nylon CFT NS-27 Polyacryl CFT PAS-27 Cotton/Polyester CFT PCS-27 With Termamyl ® With Stainzyme ® Commercial powder with bleaching agent, dosage: 4 g/l, Commercial washing machine, temperature: 40°C, short programme

53 NOVOZYMES PRESENTATION30/04/201553 Rice starch Cotton CFT CS-28 Polyester CFT PS-28 Nylon CFT NS-28 Polyacryl CFT PAS-28 Cotton/Polyester CFT PCS-28 With Termamyl ® With Stainzyme ® Commercial powder with bleaching agent, dosage: 4 g/l, Commercial washing machine, temperature: 40°C, short programme

54 NOVOZYMES PRESENTATION30/04/201554 Status for Amylases  Amylases important for stain removal in laundry  Amylases are to a large extent recognised as a provider of basic detergency in laundry  Amylases very important type of enzymes for dishwashing applications  Stainzyme launch is a break through in amylase technology

55 NOVOZYMES PRESENTATION30/04/201555 Cellulases from Novozymes Celluzyme®first cellulase from NZ, colour clarification, prevents anti-redeposition, tolerant towards LAS. Multi-component enzyme product. 1986. Carezyme®colour clarification, care cellulase, high activity on cellulose fibres, sensitive to LAS. 1998. Endolase®prevents anti-redeposition and maintain whiteness (in EU liquids). Works on soluble cellulose. 1999. Renozyme®colour clarification, robust towards anionic surfactants, cost effective cellulase

56 NOVOZYMES PRESENTATION30/04/201556 Many cellulases have a domain structure Catalytic Core Hydrophilic Linker Cellulose Binding Domain

57 NOVOZYMES PRESENTATION30/04/201557 “Cellulase” enzymes working together to degrade cellulose Carezyme / Renozyme CBH I CBH II EG R NR GG EG Endolase

58 NOVOZYMES PRESENTATION30/04/201558 Cellulase reactions  Cellulases hydrolyse b-1,4-glucose-linkages forming soluble dextrins  The substrate is water-rich amorphous cellulose In fibers this is especially found in stress points where the cellulose crystal structure is disrupted  Some cellulases are only active on soluble forms of cellulose

59 NOVOZYMES PRESENTATION30/04/201559 Removal of fuzz No Cellulase With Carezyme = Clearer Colors

60 NOVOZYMES PRESENTATION30/04/201560 Cellulase effects  Fuzz removal (Color clarification)  Depilling / anti-pilling  Soil removal (cleaning)  Anti-redeposition  Softness

61 NOVOZYMES PRESENTATION30/04/201561 Endolase® antiredeposition effect White cotton Carbon soil + Wash “Grey” cotton Without cellulase “Less grey ” cotton With cellulase

62 NOVOZYMES PRESENTATION30/04/201562 Endolase® cleaning effects UnwashedWash 1Wash 2Wash 3 No Endolase Endolase Whiteness

63 NOVOZYMES PRESENTATION30/04/201563 Status for cellulases Big difference in effects of different cellulases  Color clarification & care: Celluzyme, Carezyme and Renozyme  Anti-redeposition & Cleaning: Celluzyme and Endolase Cellulases can support both basic performance and aesthetics

64 NOVOZYMES PRESENTATION30/04/201564 Factors influencing enzyme wash performance  Detergent composition  Enzyme concentration  Temperature  pH  Water hardness  Contact time  Type of soil  Type of wash system  Type of wash method

65 NOVOZYMES PRESENTATION30/04/201565 Thank you! Slide Divider Use: Drawing attention to the different chapters or themes in the presentation Edit: Change the photo by marking the background plane (largest object) in the grouped object: > Click on Format AutoShape > Under Fill – Colour, choose Fill Effects > Go to Picture tab and click on Select Picture tab > Choose picture > Click OK Slide Divider Use: Drawing attention to the different chapters or themes in the presentation Edit: Change the photo by marking the background plane (largest object) in the grouped object: > Click on Format AutoShape > Under Fill – Colour, choose Fill Effects > Go to Picture tab and click on Select Picture tab > Choose picture > Click OK guide

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