1. Development of an integrated biorefinery for processing chitin rich biowaste to specialty and fine chemicals ChiBio is a large integrated R&D project funded through the FP7 KBBE Programme of the European Union Grant agreement no: 289284 www.chibiofp7.eu

2. Agenda Project Motive What is in crustacean waste? What is Chitin? And what are its applications? ChiBio Refinery and project PERT Chart Who is involved in this project? Work Package 2 (WP2)-Fishery Waste Pretreatment WP2 Objectives WP2 Achievements Questions

3. Fishery Waste to High Value Products The European fishery industry is a major source of industrial biowaste in Europe. The processing of crustaceans (e.g. shrimps and crabs) in the EU alone results in more than ~750.000 t/a of shell waste 1 and globally it is estimated at 1.44 million metric tons

4. Crustacean Waste Protein Calcium Carbonate Carotenoids Polysaccharides- Chitin > Chitosan

5. Chitin Naturally occurring polysaccharide, second most abundant in nature next to cellulose Chitin and its deacetylated derivative chitosan are exceptionally useful polymers They are biodegradable, non-toxic and biocompatible making them useful in a range of applications

6. Chitin Uses Agriculture- fertilizer to improve crops Medicine- Wound and burn treatment- increases healing time Food- additive to stabilise foods Water treatment, the paper and textile industries and tissue engineering

7. ChiBio Refinery

8. ChiBio-Partners Participant no. Short name Participant organisation nameCountry 1 (Coordinator) FhG Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. DE 2 TUM Technische Universität München DE 3 LIT Letterkenny Institute of Technology IE 4 UMB The Norwegian University of Life Sciences NO 5 INS Institute National des Sciences et Technologies de la Mer TN 6 APR Apronex s.r.o. CZ 7 ERF EARAGAIL EISC TEORANTA (Errigal Fish Company LTD) IE 8 EVO Evonik Industries AG DE 9 SCAG Südchemie AG DE 10 EIL Energieinstitut an der Johannes Kepler Universität Linz GmbH AT 11 PTS PT Biotech Surindo ID

9. PERT-Chart

10. WP2 Fishery Waste Pretreatment Carla Harkin Dr. Denis McCrudden Dr. Wolfram Bruck Dr. Catherine Lynch

11. WP2 Objectives Acquire crustacean shell wastes (Europe/Asia/Africa) Pretreatment of crustacean shell wastes to chitin and chitinous material (Raw/Cooked/Chemical/Microbial/Size) Stabilization for transportation (moist/dry/purity) Setting up analytical methods for constant quality control for chitin/chitinous material (Determination of optimum assays for QC)

12. WP2 QC methods established: solubility of product, water and fat solubility, degree of deacetylation, ash, FTIR, moisture, microbial load, nitrogen content (Kjeldahl), and composition of raw materials (AA). Protocols generated for chemical and microbial isolation of chitinous materials. Batches of chitinous material have been sent to the project partners fulfilling our work targets

13. QUESTIONS? References FAOSTAT, FAO statistical databases, fisheries data. 2001. Food and Agriculture organization of the United Nations, Rome, Italy, (http://www.fao.org).http://www.fao.org THANKS