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13 Oct. 2005GW Canters Leiden Univ1. Scanning Probe Microscopy (SPM) Atomic/scanning Force Microscopy Scanning Tunnelling Microscopy.

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Presentation on theme: "13 Oct. 2005GW Canters Leiden Univ1. Scanning Probe Microscopy (SPM) Atomic/scanning Force Microscopy Scanning Tunnelling Microscopy."— Presentation transcript:

1 13 Oct. 2005GW Canters Leiden Univ1

2 Scanning Probe Microscopy (SPM) Atomic/scanning Force Microscopy Scanning Tunnelling Microscopy

3 AFM resolution Chris Chan, MetProt Group, private comm. 2004

4 Mica 5nm Au nanoparticle Chris Chan, MetProt Group, private comm. 2004

5 2 & 5nm Au particles on mica, TM in water Chris Chan, MetProt Group, private comm. 2004

6 5nm Au particles on HOPG Chris Chan, MetProt Group, private comm. 2004

7 13 Oct. 2005GW Canters Leiden Univ7 J.Davis et al., Chem Communic. (2003) Advance pub.

8 13 Oct. 2005GW Canters Leiden Univ8 J.Davis Pers. Communic. (2003)

9 13 Oct. 2005GW Canters Leiden Univ9 Forcing current through a single metalloprotein Fluid conductance J.Davis Pers. Communic. (2003)

10 13 Oct. 2005GW Canters Leiden Univ10 J.D. Davis, D. Bruce, G.W. Canters, J. Crozier, H.A.O. Hill (2003) Chem. Commun Height distribution was found to be the same for the wt and K27C azurins, while the height of S118C is Å lower AFM permits to distinguish between the “standing” and the “lying” azurin molecules. Electrochemical characteristics of the azurin monolayers. Half wave potential is defined as (E pa –E pc )/2 vs. SCE Azurin WT Azurin K27C Azurin S118C E 1/2 * (mV vs. SCE)165–175165–175120–145 k et /s –1 300–400210–320300–570 BUT: the electron transfer rate is independent of the distance between the Cu and the electrode surface Is it possibly an effect of heterogeneous binding?

11 13 Oct. 2005GW Canters Leiden Univ11 Flavodoxin

12 13 Oct. 2005GW Canters Leiden Univ12 Flavodoxin N106C dimer on mica ~0.1ug/ml in 20mM MES pH6 TM in air STM images of tip-induced dissociation of K27C azurin dimer, (a) before and (b) after application of a voltage pulse. Scanning conditions were bias voltage 0.1 V, current 0.1 nA, temperature 20±1 °C, relative humidity 35±5%. Halliwell et.al Chris Chan, MetProt Group, private comm. 2004

13 The trimeric structure of copper-containing NiR MonomerTrimer

14 Nir on template stripped gold 10ug/ml in 20mM MES, pH 6 TM in air Chris Chan, MetProt Group, private comm. 2004

15 13 Oct. 2005GW Canters Leiden Univ15 Nir on template stripped gold 100ug/ml in 20mM MES, pH 6 TM in air Chris Chan, MetProt Group, private comm. 2004

16 1ug/ml Nir M94C, polylysine on mica TM in 20mM MES, pH6 Chris Chan, MetProt Group, private comm. 2004

17 13 Oct. 2005GW Canters Leiden Univ17

18 13 Oct. 2005GW Canters Leiden Univ18 Nature Reviews Mol. Cell Biol. 3(2002)906 JACS 124(2002)6063 PNAS 101(2004)523

19 13 Oct. 2005GW Canters Leiden Univ19 Nature Reviews Mol. Cell Biol. 3(2002)906


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