Presentation on theme: "Introduction to the Laboratories of Bio and Soft-matters 陳志強 中央研究院物理研究所 中央大學生物物理研究所."— Presentation transcript:
Introduction to the Laboratories of Bio and Soft-matters 陳志強 中央研究院物理研究所 中央大學生物物理研究所
Content Overview of Biophysics in Academia Sinica (15 min) Laboratory of Soft and Biomatter (30 min) Effects of Conformations of DNA on some Physical and Biological Problems Communications in Excitable Networks
Institute of Physics Academia Sinica Theory / High Energy Nano/Surface Complexity
Research Opportunities in Academia Sinica 李世炳 - System Biology 梁鈞泰 - Flocking 周家復 - Nano/Bio devices 林耿慧 - Self-Assembly 阮文滔 - Single molecule DNA 陳彥龍 - Single DNA in Microfluidics 李定國 - Protein folding 胡進錕 - Protein folding, DNA and etc
Laboratory of Bio and Softmatters Softmatter -- de Gennes in the 90s Energy comparable to KT, many degree of freedom (complexity) Nonlinearity and Cooperativity Polymers - proteins, DNA Networks - polymers, bio-chemical reactions, cells and neurons
Why DR with DNA ? 1.DNA is very long - can be much longer than 2. DNA processes secondary structure - elastic properties different from polymers 3. Structure can be changed by pH or temperature - Good probe for turbulence ?
Setup (1) Motor (2) Flexible couple ring (3) Torque sensor (4) Fluid container (5) Rotating disk (1) (2)(3) (5) (4) Lambda DNA - Promega Corporation / U.S.A. - Size: 48,502bp - Commonly used substrate in restriction enzyme activity assay. - Storage: –20 ℃ - Concentration of stock solution: 515 g/ml Buffer solution - 10mM tris-HCL (pH 7.8 at 25 ℃ ), 10mM NaCl and 1mM EDTA.
Torque Measurement The Shaft torque measurement for buffer solution and 1.35 wppm solution of lambda DNA in continuous mode rpm sweep at 25 ℃. (sweep rate; 20rpm/sec) Torque Variation
Only L/2 degradation The electrophoresis result of lambda DNA after degradation for 1 hour at 1,980 rpm, 25 ℃. Electrophoresis 23.1Kp
Summary of DR Only a few %DR by improvement of wall properties a few ppm of additive of long chain polymers in turbulent flow can produce a max of 80% DR Polymers broken (degraded) by turbulence NO BASIC UNDERSTANDING YET ! DNA might provide a good probe for turbulence
Summary Many nonlinear effects induced by the conformation of DNA Turbulent Drag Reduction Coil-Globule Transition Structure and Packing of DNA Effect of conformation of the transcription of DNA
Complexity from Bio-matters Communications in Excitable Networks
Scale-free network Poisson distribution Exponential Network Power-law distribution Scale-free Network Airlines Highway
Summary Biophysics Understanding of Biological Nonlinearity and Complexity in terms of Physical Principles Physics of Polymers and Networks of Excitable Systems DNA Slime Mould Heart Brains Both fundamental and applied problems NCU/Academia Sinica Best Combination in Taiwan