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DISCOVERY OF GIBBERELLINS 1.“BAKANAE” OR “FOOLISH SEEDLING” DISEASE OF RICE : plants grew too talkk and toppled over. 2.JAPANESE SCIENTISTS WORKED ON.

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Presentation on theme: "DISCOVERY OF GIBBERELLINS 1.“BAKANAE” OR “FOOLISH SEEDLING” DISEASE OF RICE : plants grew too talkk and toppled over. 2.JAPANESE SCIENTISTS WORKED ON."— Presentation transcript:

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2 DISCOVERY OF GIBBERELLINS 1.“BAKANAE” OR “FOOLISH SEEDLING” DISEASE OF RICE : plants grew too talkk and toppled over. 2.JAPANESE SCIENTISTS WORKED ON THE PROBLEM AND IDENTIFIED CAUSE: INFECTION BY FUNGUS, GIBBERELLA FUJUKUROI. 3.MID 1950s: ACTIVE SUBSTANCE CRYTALIZED AND IDENTIFIED IN ENGLAND AND US: GIBBERELLIC ACID (GA 3 )

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7 Rice leaf sheath bioassay for gibberellin Increasing GA 3 concentration

8 GIBBERELLIN BIOSYNTHESIS AND METABOLISM 1.GIBBERELLINS ARE SYNTHESIZED VIA THE TERPENOID PATHWAY. 2. BASED ON ISOPRENE UNITS: 3.BIOLOGICAL ISOPRENE UNIT = ISOPENTENYL DIPHOSPHATE

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12 LOCATIONS OF SOME MUTANT GENES THAT BLOCK GA BIOSYNTHETIC PATHWAY IN PEA

13 GA 1, THE BIOLOGICALLY ACTIVE GIBBERELLIN, IS SYNTHESIZED FROM GA 20

14 GA 1 LEVELS CORRELATE WITH INTERNODE LENGTH

15 Phenotypes and genotypes of peas with different gibberellin levels

16 Luciferase reporter construct for GA 20-oxidase HIGHEST IN APICAL BUD

17 EFFECT OF AMO-1618 ON PETIOLE ELONGATION OF SPINACH

18 EFFECT OF LONG DAYS ON GA LEVELS IN SPINACH

19 Long DaysShort Days Effect of Long Days (High GA levels) on Tuber Formation in Potato

20 Genetically Engineered Dwarf Wheat Plants Overexpressing GA 2-ox, which degrades GA 1

21 KINETICS OF GA-INDUCED ELNGATION OF DEEPWATER RICE INTERNODE 30 min

22 DISTRIBUTION OF CELL DIVISION FOLLOWING GA TREATMENT OF A ROSETTE PLANT, Samolus parviflorus

23 Arabidopsis GIBBERELLIN RESPONSE MUTANTS WITH DEFECTS IN SIGNAL TRANSDUCTION 1. Gibberellin-in sensitive dwarfs 2. Gibberellin-deficient mutants in which GA deficiency has been overcome by a second suppressor mutation, so plant looks normal (double mutant) 3. Mutants with constitutive gibberellin response (“slender” mutants)

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25 GAI and RGA are related repressor proteins Repression of GA Response Genes GA Response Genes Expressed

26 DIFFERENT TYPES OF MUTATIONS OF GAI AND RGA REPRESSORS AFFECT GROWTH DIFFERENTLY

27 Enzyme involved in protein modification - enhances activity of GAI and RGA GA acts to block action of both SPY and GAI/RGA

28 THE CEREAL ALEURONE LAYER

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34 EFFECT OF GA ON CALCIUM IN BARLEY ALEURONE LAYER PROTOPLAST CONTROL + GA 3 GA 3 + ABA (ABSCISIC ACID) Low Cytoplasmic [Ca2+] High

35 SUMMARY OF STEPS IN GA SIGNAL TRANSDUCTION PATHWAY


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