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Genetic Engineering in Plants — Transgenic Plants 第十章 植物的基因工程
Agrobacterium tumefaciens and Crown galls
Transformation of plant cells byAgrobacterium tumefaciens
Agrobacteria cause crown gall tumors in plants
Structure of the octopine and nopaline plasmids
Structure of the nopaline Ti plasmid pTi C58 LB---left border RB---right border
Map of the nopaline T-DNA region
Formation of a cointegrate Ti plasmid
Binary vector system baced on Bin19
A GUS vector CaMV-cauliflower mosaic virus GUS-glucuronidase NPTII---neomycin phospho- trasferase II
Regeneration of transgenic plants by leaf discs
Regeneration of plants from protoplasts Auxins/cytokinins↑, Root development Auxins/cytokinins↓, Shoot development
Regeneration of leaf disks infected by Agrobacterium
Transfer of T-DNA from Agrobacterium in to a plant cell---Vir proteins and T-DNA transfer
Transfering genes into plant cells by cointegration using T-DNA NPTII---neomycin phospho- trasferaseII
Transfer of an antisense gene for polygalacturonase (PG) into tomato cells by using a binary vector CaMV-cauliflower mosaic virus GUS-glucuronidase
The antisense RNA procedure for blocking or reducing the level of expression of a specific gene
The regulation of biosynthesis of Ethylene (ETH)
反义基因在耐储藏番茄育种中的应用 ACC gene ( 1- 氨基丙环烷羧酸氧化酶， 乙烯合成途径酶类 ) anti-sense ACC-mRNA ACC-mRNA 乙烯合成被抑制 (-) 5 3mRNA (-) 5 3 (+) 3 5 cDNA anti-sense ACC gene pCaMV35s-
耐储藏番茄 华番一号 我国第一个基因工程农作物品种
Systemic infection of a plant with recombinant geminivirus Vectors Gene A---Replication in plant cells Gene B---Infectivity
Direct transfer of DNA into plant cells by microprojectile bombardement (shotgun)
Stable transformation of plastids in tobacco plants Sp---spectinomycin Sm---streptomycin
Cloning a plant gene by transposon tagging
Cloning plant genes by T-DNA insertional Mutagenesis EMS---ethylmethane sulfonate
Protection of plants from tobacco mosaic virus (TMV) infection by transgenic expression of the TMV coat protein(CP)
Established Bt and Roundup ready crops
Killing insect larvae with transgenic cotton plants that express the Bacillus thuringiensis(Bt) toxin gene
苏云金芽胞杆菌（ BT ）毒素蛋白基因
苏云金芽胞杆菌（ BT ）毒素蛋白基因 转 BT 基因水稻抗稻纵卷叶螟 抗病基因＋抗虫基因＋优质基因
Engineering herbicide-resistance plants
Roundup Ready® 转抗除草剂基因的 大豆和玉米
Plants as bioreactors to produce antibodies
Chapter 18-Genetic Engineering of Plants: Methodology Plant transformation with the Ti plasmid of Agrobacterium tumefaciens Ti plasmid derived vector systems.
Genetic Engineering of Plants BIT 220 End of Chapter 22.
Ti- 质粒与植物基因组的相互作用 蒋舜媛 董霞 任昶 DNA 重排研究进展专题.
Unit II Lecture 4 B. Tech. (Biotechnology) III Year V th Semester EBT-501, Genetic Engineering.
Plant Genetic Engineering. Genetic Engineering The process of manipulating and transferring instructions carried by genes from one cell to another Why.
Plant Genetic Transformation. All stable transformation methods consist of three steps: Delivery of DNA into a single plant cell. Integration of the DNA.
High efficiency transformation of tall fescue with Agrobacterium tumefaciens Shujie Dong, Rongda Qu * Plant Science 168 (2005) 1453–1458 生科四甲 李廷原
Molecular Basis for Relationship between Genotype and Phenotype DNA RNA protein genotype function organism phenotype DNA sequence amino acid sequence transcription.
Plant transformation Introduction of individual gene(s) of interest into plant genome Genetic modification with or without integration May include regeneration.
基因工程技术向 “ 肥胖 ” 宣战 瘦蛋白（ Leptin) 基因 克隆与表达 瘦蛋白的基因工程.
PLANT VECTORS REKHA PULICHERLA. Plasmids A circular piece of DNA that is not a part of the bacteria’s genome. It is ‘extra-chromosomal.’ Analogy: The.
The Basics of Transgenic Technology Pat Byrne Department of Soil & Crop Sciences Colorado State University.
C H A P T E R PowerPoint ® Lecture by: Lisa Werner Pima Community College Plant Biotechnolog y 6.
Copyright © 2009 Pearson Education, Inc. PowerPoint Lectures for Introduction to Biotechnology, Second Edition William J.Thieman and Michael A.Palladino.
Plant Biotechnology Chapter 6. Motivation for genetically engineered crops Agriculture is the biggest industrial sector in the world –$1.3 trillion of.
GENE DELIVERY TECHNIQUES. Genetic Engineering of Plants Must get DNA: 1.into the cells 2.integrated into the genome 3.Expressed For (1) and (2), two main.
RECOMBINANT DNA TECHNOLOGY PART 2 Topics Genomic libraries Id a specific clone or sequence within a library Transgenic plants Transgenic animals Timely.
Transfection-Transfer of non-viral genetic material into eukaryotic cells. Infection/ Transduction- Transfer of viral genetic material into cells.
Additional Transformation Topics. Chloroplast Transformation.
Agricultural Biotechnology Altering Genes in Plants to Fight Pests and Improve Nutrition Altering Genes in Plants to Fight Pests and Improve Nutrition.
Genetically Modified Organisms (GMOs) Any microorganism, plant or animal that has purposely had its genome altered using genetic engineering technology.
Chapter 6 Plant Biotechnology. Plant Structure CO 2 + H 2 O →C 6 H 12 O 6 + O 2 Plant Structure.
Plant Molecular Genetics and Genetic Transformation HORT 301 – Plant Physiology November 1, 2010 Taiz and Zeiger – Chapter 2, Smith et al. (2010) – Chapter.
Biotechnology in Agriculture Chapter 11. Learning Outcomes Describe the role of meristematic tissue in propagating plants by various asexual methods.
Dr. Sanjay Singh C.M.P. College Allahabad Seminar presented at Department of Botany, University of Allahabad, Allahabad on
Vaccines, Clones &Transgenics. Plant vaccines contain dead or weakened strains of plant virus which are injected to plants against diseases Vaccines.
What is Biotechnology?. General Definition The application of technology to improve a biological organism Detailed Definition The application of the technology.
Transgenic Plants. Prepare tissue for transformation Introduce DNA Culture plant tissue Develop shoots Develop Roots Field test the plants Leaf, germinating.
RT-PCR 扬州大学 生物科学与技术学院. 背景介绍 DNA 存在于细胞核中并编码了基因 转录 : 双链 DNA 解链后利用其中一条链（编 码链）合成信使 RNA （ mRNA ） mRNA 从细胞核转移到细胞质中 mRNA 结合上核糖体开始翻译成蛋白质 蛋白执行基因的功能.
Genetic Engineering of Plants Roman Sobotka. Terminology matters... Plant breeding (even conventional like selectively mating plants) is a kind of genetic.
1 Regulation of gene expression prokaryotes eukaryotes gene activity is regulated primarily at the level of transcription.
Lecture 9 &10 Insect resistant plants. Genetic engineering of plants and applications.
Science and GMO-relevant technology Genes and genomes – last week –Genomes and their inheritance and variation –Genes and their structure –Important methods:
Genetically Modified Organisms - plants, animals, microorganisms - add or take away traits.
第九章 核糖体 Robinson ＆ Brown （ 1953 ）发现于植物 细胞。 Palacle （ 1955 ）发现于动物细胞。 Roberts （ 1958 ）建议命名为核糖核蛋白 （ ribosome ），简称核糖体。 核糖体是细胞内合成蛋白质的工厂，在 一个旺盛生长的细菌中，大约有
Genetically Modified Organisms Image:
Plant Genetic Engineering. 1.a suitable transformation method 2.a means of screening for transformants 3.an efficient regeneration system 4.genes/constructs.
Lecture 15 Chapter 10 Plant transformation II Neal Stewart.
第二章 病毒的复制 病毒在活细胞内，以其基因为模板，在酶的作用下，分别 合成病毒基因及蛋白质，再组装成完整的病毒颗粒，这种 方式称为复制（ replication) 。 从病毒进入宿主细胞开始，经过基因组复制和病毒蛋白合 成，至释放出子代病毒的全过程，称为一个复制周期。 病毒的复制周期主要包括吸附和穿入、脱壳、生物合成、
Chapter 6 Plant Biotechnology. Agriculture: The Next Revolution Biggest industry in the world ($1.3 trillion of products per year) Plant transgenesis.
Unit 1 Cell and Molecular Biology Section 10 Agriculture.
1 Transgenic Animals and Plants - Genetic Engineering of plant -> Transgenic plants - Genetic Engineering of animals -> Transgenic animals.
Agriculture Transgenic Plants Production of Bovine Somatotrophin.
Genetic Engineering of Plants Must get DNA: 1.into the cells 2.integrated into the genome (unless using transient expression assays) 3.expressed (everywhere.
DNA technology Ch. 10 Cloning genes Into bacteria Into plants Into animals.
第 3 章 细胞的基本结构 第 2 节 细胞器 —— 系统内的分工合作. 细胞的结构 细胞质基质 细 胞 器细 胞 器.
Biotechnology and Agriculture Madam Noorulnajwa Diyana Yaacob Universiti Malaysia Perlis LECTURE 10 & 11.
Genetically Modified (GM) Foods GM foods can be either transgenic or cisgenic. The transgene codes for a protein that is somehow advantageous to the plant.
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