We think you have liked this presentation. If you wish to download it, please recommend it to your friends in any social system. Share buttons are a little bit lower. Thank you!
Presentation is loading. Please wait.
Published byAmani Mussett
Modified about 1 year ago
MES-4 Chromatin Regulation in the C. elegans Germ Line
XX XO soma both Xs are reduced ~50% germline Xs are not transcribed Dosage compensation in worms:
How chromatin is organized…
The Histone Code H3 H4 N N H3 N N NN acetylated Lys 8, 12, 16 activating methylated Lys 9 silencing methylated Lys 4, 36 activating methylated Lys 27 silencing methylated Lys 20 silencing
Ref. Get better resolution! Kornberg and Lorch, Cell 1999 H3 H4 H2A H2B The nucleosome.
MES-4 binds to just 5 of the 6 chromosomes. MES-4 + DNA DNA alone maternal pronucleus paternal pronucleus
WT mes-4 WT mes-4 2mK36 H3-Lys36 di-methylation is on Xs in early embryos. MES-4 is required for H3-Lys36 di-methylation. 1-cell
Antibody Use in Detection of Target Protein
NSD1 catalyzes H3 Lys36 and H4 Lys20 methylation in vitro. (Raysam et al., EMBO J 2003) MES-4 and its homologs
C. elegans (MES-4) and C. briggsae (CBG60706) Homologs
MES-4 and CBG60706
CB60706 and MES-4 Introns and Exons
BLAST Amino Acid Alignment Under Default Settings
Genes & Chromosomes Chromosomes - single large DNA molecule and its associated proteins, containing many genes; stores & transmits genetic info Gene -
The Code of Life: Topic 4 Regulation of gene expression.
Control of Gene Expression Eukaryotes. Eukaryotic Gene Expression Some genes are expressed in all cells all the time. These so-called housekeeping genes.
Homework: Lab 6B Analysis Questions – due tomorrow Problem Set will be due next Wednesday Do Now: With your lab group… Take out lab packet
X-inactivation 20/11/08. Males are haploid for X-linked genes expression.
X-inactivation. Males are haploid for X-linked genes.
Nucleic acid Basics Hybridization Electrophoresis PCR Diagnostic tools DNA-Protein interactions Chromatin Gene expression.
Control of Gene Expression. The Central Dogma From DNA to Proteins DNA RNA Protein Translation Transcription Genotype Phenotype.
Lecture #8Date _________ n Chapter 19~ The Organization and Control of Eukaryotic Genomes.
Eukaryotic Genomes 11 November, 2005 Text Chapter 19.
Salivary gland- polytene chromosomes Brain-diploid metaphase chromosomes Same Objective Lens: Why are the salivary gland chromosomes so much larger? Alyssa.
DNA and RNA Chapter 12 JEOPARDY #2 S2C06 Jeopardy Review
Ch 15 -.Gene Regulation Prokaryote Regulation Operon * not found in eukaryotes Operon * not found in eukaryotes Regulator gene = codes for repressor.
Advantages of C. elegans: 1. rapid life cycle 2. hermaphrodite 3. prolific reproduction 4. transparent 5. only ~1000 cells 6. laser ablation 7. complete.
MCDB 4650 Developmental Control of Gene Expression.
© 2015 Pearson Education, Inc. Chromosome structure and chemical modifications can affect gene expression DNA packing.
Fig Enhancer (distal control elements) Proximal control elements Poly-A signal sequence Termination region Downstream Promoter Upstream DNA Exon.
© 2012 Pearson Education, Inc. Lecture by Edward J. Zalisko PowerPoint Lectures for Campbell Biology: Concepts & Connections, Seventh Edition Reece, Taylor,
Gene Expression Expression of different set of genes in each cell type.
Eukaryotic Gene Regulation. Chromatin Structure DNA & protein 1) Nucleosomes DNA & histones (proteins) DNA wrapped around 8-piece histone bead.
Regulation of Gene Expression Eukaryotes. I. Regulation at Stages A. All organisms prokaryotes and eukaryotes alike have to regulate which genes are expressed.
Eukaryotic Gene Expression. Introduction Every cell in a multi-cellular eukaryote does not express all its genes, all the time (usually only 3-5%) –Long-term.
AP Biology Control of Eukaryotic Genes.
Eukaryotic Gene Regulation. 5 mechanisms 1.Chromatin Structure 2.Transcriptional 3.Posttranscriptional 4.Translational 5.Posttranslational.
REGULATION OF GENE EXPRESSION Prokaryotes vs. Eukaryotes.
How is gene expression in eukaryotes accomplished ?
Information Pathways Genes and Chromosomes CH353 March 25, 2008.
Section 8.6: Gene Expression and Regulation Biology.
DNA PACKAGING. 8 histones make up the nucleosome core DNA wraps twice around the 8 histones Histone 1 helps maintain the nucleosome DNA is negatively.
Twenty-five years of the nucleosome Kornberg and Lorch 1998, Cell 98: 285.
Gene Expression. Cell Differentiation Cell types are different because genes are expressed differently in them. Causes: Changes in chromatin structure.
GENE REGULATION RESULTS IN DIFFERENTIAL GENE EXPRESSION, LEADING TO CELL SPECIALIZATION Eukaryotic DNA.
Introduction Mitosis is a series of events that produce two identical cells from one parent cell. Mitosis only involves somatic or body cells that contain.
All the cells in the organism have the same DNA DNA is packed together with histones and other proteins into chromatin. Chromatin is a highly dynamic.
Describe the structure of a nucleosome, the basic unit of DNA packaging in eukaryotic cells.
Chapter 15 Methylation, Acetylation and Epigenetics.
Copyright © 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Differential Gene Expression Almost all the cells in an organism are genetically.
Fundamentals of Biochemistry Third Edition Fundamentals of Biochemistry Third Edition Chapter 28 Regulation of Gene Expression Chapter 28 Regulation of.
Chapter 16 The Genetic Basis of Development. Determination is a multistep process Pluripotent embryonic cells MesodermEctoderm Nerve cells Skin cells.
Chromatin Remodeling DNA is wrapped around histones to form nucleosomes DNA is wrapped around histones to form nucleosomes Chromosome packaging Chromosome.
Organism Estimated size (in bases) Estimated gene # Average gene density/base Diploid chromosome # Human2.9 x 10 9 ~30,0001/100,00046 Rat2.8 x 10 9 ~30,0001/100,00042.
GENETICS A Conceptual Approach FIFTH EDITION GENETICS A Conceptual Approach FIFTH EDITION Benjamin A. Pierce CHAPTER 17 Control of Gene Expression in Bacteria.
32 Gene regulation in Eukaryotes. Lecture Outline 11/28/05 Gene regulation in eukaryotes –Chromatin remodeling –More kinds of control elements Promoters,
Chapter 13 DNA Repair and Chromosome Structure. You Must Know DNA proofreading and packaging.
10 m mircotubules DNA Eukaryotic cell nucleus heterochromatineuchromatinnucleolus.
DNA MODIFICATIONS AND LONG-TERM PATTERNS OF GENE EXPRESSION EPIGENETICS PART 1 Feb 19, 2015.
AP Biology Discussion Notes Monday 3/14/2016. Goals for Today Be able to describe regions of DNA and how they are important to gene expression in Bacteria.
Fig. 18-3b-2 (b) Tryptophan present, repressor active, operon off Tryptophan (corepressor) No RNA made Active repressor mRNA Protein DNA.
Chromatin Immunoprecipitation and the Chip on Chip technique. Fredrik Fagerström Billai B E A- Core Facility for Bioinformatics and Expression Analysis.
© 2017 SlidePlayer.com Inc. All rights reserved.