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MES-4 Chromatin Regulation in the C. elegans Germ Line.

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Presentation on theme: "MES-4 Chromatin Regulation in the C. elegans Germ Line."— Presentation transcript:

1 MES-4 Chromatin Regulation in the C. elegans Germ Line

2 XX XO soma both Xs are reduced ~50% germline Xs are not transcribed Dosage compensation in worms:

3 How chromatin is organized…

4 The Histone Code H3 H4 N N H3 N N NN acetylated Lys 8, 12, 16 activating methylated Lys 9 silencing methylated Lys 4, 36 activating methylated Lys 27 silencing methylated Lys 20 silencing

5 Ref. Get better resolution! Kornberg and Lorch, Cell 1999 H3 H4 H2A H2B The nucleosome.

6 MES-4 binds to just 5 of the 6 chromosomes. MES-4 + DNA DNA alone maternal pronucleus paternal pronucleus

7 WT mes-4 WT mes-4 2mK36 H3-Lys36 di-methylation is on Xs in early embryos. MES-4 is required for H3-Lys36 di-methylation. 1-cell

8 Chromatography

9 Sucrose Gradients

10 Gel Electrophoresis

11 Antibody Use in Detection of Target Protein

12 NSD1 catalyzes H3 Lys36 and H4 Lys20 methylation in vitro. (Raysam et al., EMBO J 2003) MES-4 and its homologs

13 C. elegans (MES-4) and C. briggsae (CBG60706) Homologs

14 MES-4 and CBG60706

15 CB60706 and MES-4 Introns and Exons

16 Nucleotide Alignment

17 BLAST Amino Acid Alignment Under Default Settings


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