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René Wijffels, Michel Eppink, Maria AlgaePARC biorefinery.

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Presentation on theme: "René Wijffels, Michel Eppink, Maria AlgaePARC biorefinery."— Presentation transcript:

1 René Wijffels, Michel Eppink, Maria AlgaePARC biorefinery

2 Contents  Technical and economical feasibility  Process ● Photobioreactors ● Lipids ● Biorefinery  Projects ● AlgaePARC ● AlgaePARC biorefinery ● SPLASH ● FUEL4ME ● MIRACLES

3 Can algae replace commodities of today?  European market: ● Fossil fuels: 400 Mtonnes/year ● Polymers and chemicals: 100 Mtonnes/year ● Palm oil: 7 Mtonnes/year ● Soy: 8 Mtonnes/year  Global microalgae market today: ● < 10,000 tonnes/year

4 Develop this process is a sustainable and economical way within the next years To develop a more sustainable and economically feasible process, all biomass components should be used

5 From basic to applied research  Resources ● Efficient use of sunlight ● Reduction of energy input ● Use of residual nutrients  Strains ● Lipid accumulation ● Metabolic Engineering  Implementation ● Scale-up ● Biorefinery ● Design scenarios/LCA’s

6 Photobioreactor design

7 Antenna size mutants  Wild type cells ● Cells oversaturated: heat dissipation  Antenna size mutants ● Light-saturation at higher light intensity ● Less energy wasted. Tim de Mooij Turbidostat

8 Behaviour during day/night cycles The aim of this research is to obtain more knowledge in the circadian behaviour of the cell cycle of lipid producing microalgae grown in photobioreactors. This will provide ways to optimize the current production process under outdoor day/night cycles. Neochloris oleoabundans is grown in a lab-scale photobioreactor under simulated outdoor conditions. The circadian rhythm of the cell cycle is analysed using flow cytometry. In addition, cell composition (lipids, starch, proteins) during the cell cycle is investigated. Lenneke de Winter

9 Lipids e e e e e e e e e e

10 Lipid accumulation N+ N- Breuer G., Lamers P.P., Martens D.E., Draaisma R.B., Wijffels R.H. (2012) The impact of nitrogen starvation on the dynamics of triacylglycerol accumulation in nine microalgae strains. Bioresource Technology 124:

11 Genetic engineering  Lipids ● Strain improvement towards a desired fatty acid profile ● Strain improvement towards a increased TAG production  Hydrocarbons ● Botryococcus ● Insert Botryococcus genes for hydrocarbons in Chlamydomonas  Mutagenesis and direct gene targeting approaches  Transcriptomics to obtain insight in regulation Stefan Aanstoot Lenny de Jaeger

12 TAG production: process optimization Breuer et al. 2013

13 Continuous TAG production Klok et al Anne Klok NO 3 N- medium Different light:N ratios

14 Richard Postma Proteins from Green Sources  Development of a mild, continuous, scalable disruption and protein extraction technology with minimal energy requirements Soluble Insoluble Disruption - Bead-milling (reference method) - Enzymatic - Pulsed Electronic Field Extraction - Ionic liquid - Polymer precipitation - Ultrafiltration

15 AlgaePARC  Basic research is not sufficient  Gap between basic research and commercial applications  Continuous interaction between basic research and pilots  Follow up in demonstrations  Products  Scale  Production chain

16 AlgaePARC  International centre of applied research  Intermediate between basic research and applications  Development of competitive technology (economics, sustainability)  Acquire information for full scale plants  Algal biomass for food, feed, chemicals and fuels

17 Scenario: Lipids + Biogas energy Scenedesmus: €600/T Cost of production: €500/T

18 Scenario: Lipid & protein biorefinery Nannochloropsis: €8,000/T Cost of production: €500/T

19 AlgaePARC biorefinery  Fractionate all components  Maintain their functionality  At low energy input

20 EU-FP7 projects  INTESUSAL: demonstration project Portugal  ALGADISK: biofilmreactor; pilot Spain  FUEL4ME: biofuel project with pilots in Israël, Italy, Spain and Netherlands  SPLASH: biomaterials project with pilots in Spain and Netherlands  MIRACLES: biorefinery project for food and feed applications with pilots in Norway, Chile, Netherlands and Spain

21 Education  Primary school  High schools ● Design challenge and practical kit  BSc ● Physiology and process design  MSc ● Bioreactor design  PhD ● Designing of microalgae-based production processes, for research and commercial purposes ● Next edition: June 2014

22 Algae for commodities  Demonstrate that biobased products have the quality to replace existing products  Convince we can produce economically in the future  Imagine we can reach the right market volumes  Create awareness: it is not going to be easy  Join forces  Create a long term commitment

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