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AlgaePARC biorefinery

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Presentation on theme: "AlgaePARC biorefinery"— Presentation transcript:

1 AlgaePARC biorefinery
René Wijffels, Michel Eppink, Maria Barbosa @ReneWijffels

2 Contents Technical and economical feasibility Process Photobioreactors
Lipids Biorefinery Projects AlgaePARC AlgaePARC biorefinery SPLASH FUEL4ME MIRACLES

3 Can algae replace commodities of today?
European market: Fossil fuels: Mtonnes/year Polymers and chemicals: 100 Mtonnes/year Palm oil: Mtonnes/year Soy: Mtonnes/year Global microalgae market today: < 10,000 tonnes/year

4 Develop this process is a sustainable and economical way within the next 10 -15 years
To develop a more sustainable and economically feasible process, all biomass components should be used

5 From basic to applied research
Resources Efficient use of sunlight Reduction of energy input Use of residual nutrients Strains Lipid accumulation Metabolic Engineering Implementation Scale-up Biorefinery Design scenarios/LCA’s

6 Photobioreactor design

7 Antenna size mutants Wild type cells
Cells oversaturated: heat dissipation Antenna size mutants Light-saturation at higher light intensity Less energy wasted. Tim de Mooij Turbidostat

8 Behaviour during day/night cycles
Lenneke de Winter The aim of this research is to obtain more knowledge in the circadian behaviour of the cell cycle of lipid producing microalgae grown in photobioreactors. This will provide ways to optimize the current production process under outdoor day/night cycles. Neochloris oleoabundans is grown in a lab-scale photobioreactor under simulated outdoor conditions. The circadian rhythm of the cell cycle is analysed using flow cytometry. In addition, cell composition (lipids, starch, proteins) during the cell cycle is investigated.

9 Lipids e e e e e

10 Lipid accumulation N- N+
Nauwelijks TAG onder groeicondities zonder stikstof limitatie Alle algen hopen TAG op maar sommige niet veel 6 algen hopen veel vet op, en 4 zelfs meer dan 35% TAG Hoeveelheid functionele vetten (vetzuren) neemt af onder stikstof limitatie Breuer G., Lamers P.P. , Martens D.E. , Draaisma R.B., Wijffels R.H. (2012) The impact of nitrogen starvation on the dynamics of triacylglycerol accumulation in nine microalgae strains. Bioresource Technology 124:

11 Genetic engineering Lipids
Strain improvement towards a desired fatty acid profile Strain improvement towards a increased TAG production Hydrocarbons Botryococcus Insert Botryococcus genes for hydrocarbons in Chlamydomonas Mutagenesis and direct gene targeting approaches Transcriptomics to obtain insight in regulation Stefan Aanstoot Lenny de Jaeger

12 TAG production: process optimization
Breuer et al. 2013

13 Continuous TAG production
Different light:N ratios Anne Klok NO3 N- medium Klok et al. 2013

14 Richard Postma Proteins from Green Sources
Development of a mild, continuous, scalable disruption and protein extraction technology with minimal energy requirements Soluble Insoluble Disruption Bead-milling (reference method) Enzymatic Pulsed Electronic Field Extraction Ionic liquid Polymer precipitation Ultrafiltration

15 AlgaePARC Basic research is not sufficient
Gap between basic research and commercial applications Continuous interaction between basic research and pilots Follow up in demonstrations Products Scale Production chain

16 AlgaePARC International centre of applied research
Intermediate between basic research and applications Development of competitive technology (economics, sustainability) Acquire information for full scale plants Algal biomass for food, feed, chemicals and fuels

17 Scenario: Lipids + Biogas energy
Scenedesmus: €600/T Cost of production: €500/T

18 Scenario: Lipid & protein biorefinery
Nannochloropsis: €8,000/T Cost of production: €500/T

19 AlgaePARC biorefinery
Fractionate all components Maintain their functionality At low energy input

20 EU-FP7 projects INTESUSAL: demonstration project Portugal
ALGADISK: biofilmreactor; pilot Spain FUEL4ME: biofuel project with pilots in Israël, Italy, Spain and Netherlands SPLASH: biomaterials project with pilots in Spain and Netherlands MIRACLES: biorefinery project for food and feed applications with pilots in Norway, Chile, Netherlands and Spain

21 Education Primary school High schools
Design challenge and practical kit BSc Physiology and process design MSc Bioreactor design PhD Designing of microalgae-based production processes, for research and commercial purposes Next edition: June 2014

22 Algae for commodities Demonstrate that biobased products have the quality to replace existing products Convince we can produce economically in the future Imagine we can reach the right market volumes Create awareness: it is not going to be easy Join forces Create a long term commitment

23 @ReneWijffels

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