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**Biotest Medizintechnik GmbH**

Hemoglobin determination Biotest Hemoglobin System Accuracy and precision Biotest Medizintechnik GmbH

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**Products Cuvette Measuring device Biotest Hemoglobin**

Biotest Hemoglobin Tester

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**Function Cuvette Biotest Hemoglobin Tester**

Blood capillary, reagent and measuring cuvette in one. Biotest Hemoglobin Tester Photometer with integrated control and digital display

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**Function - Cuvette Chemical reaction Haemolysis by sodium desoxycholat**

Sodium nitrite Sodium azid Methemo-globin Free Haemoglobin Azid- Methaemo-globin Erythrocytes

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Function - Cuvette Absorbance curve

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Measurements

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Measurements Linearity and precision, n = 20 measurements by each blood sample. y = 1,0064x - 0,0773 R² = 0,9992 5,00 10,00 15,00 20,00 5,0 g/dl 10,0 g/dl 15,0 g/dl 20,0 g/dl Biotest Hemoglobin System, g/dl Mean value HiCN, i g/dl

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Measurements

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**Function - Tester Photometer; two wave lengths**

Evaluation and calculation Reading Evaluation

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**Self calibration 100% green light, 100% red light and dark current**

Compared to moment of calibration If necessary automatic adjustment of LED current Evaluation

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**Resolution Max deviation = +/- 0,75 % or +/- 30 steps Absorption**

256 g/L 0 = 100 % light 470 – 880 nm 12 bits = 4000 ”steps” Resolution

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**Calibration Absorption 256 g/L**

Two points of calibration for linearity; 80 and 220 g/L Three control levels for linearity 120, 150, 180 g/L 0 = 100 % light 470 – 880 nm 12 bits = 4000 ”steps”

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**Function - Tester Calculation of Hb Extinction Turbidity Turbidity**

Wave length [nm]

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Capillary blood Hb Time 0 min 1 min 2 min 3 min

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**Results - Introduction**

Accuracy How close to true value is the result Precision How close to each other are repeated measurements with the same sample

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**Results - Introduction**

Accuracy and precision High accuracy Low precision Low accuracy High precision High accuracy High precision

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**Results - Introduction**

Accuracy: Deviation (mean) in g/L Preferred; deviation in % Precision: Standard deviation SD Preferred; Coefficient of variation, CV %

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**Results Precision with control blood (n=50) Mean value 12,39 g/dl**

30 Mean value 12,39 g/dl SD 0,084 g/dl CV 0,68 % 25 20 Numbers 15 10 5 12,1 12,2 12,3 12,4 12,5 12,6 12,7 Result [g/dl]

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Features Cuvette Aimed for Hb-measurement with Biotest-Hemoglobin-Tester and compatible instruments High accuracy and precision Can be used for venous, capillary and arterial blood Developed to be price worth due to latest production and control technique

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Features Tester Automatic calibration: no calibration or test cuvette needed Cuvette holder minimizing the risk of contamination Automatic monitoring of power supply with fast loading of batteries (1 hour loading > 40 hours of measuring) Automatic shut off with latest value saved Measures only cuvettes with properly haemolysed blood

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**Summary High accuracy and precision Easy and reliable system**

Long time use on battery power

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**Results - Introduction**

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