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Hemoglobin determination Biotest Hemoglobin System Accuracy and precision Biotest Medizintechnik GmbH.

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Presentation on theme: "Hemoglobin determination Biotest Hemoglobin System Accuracy and precision Biotest Medizintechnik GmbH."— Presentation transcript:

1 Hemoglobin determination Biotest Hemoglobin System Accuracy and precision Biotest Medizintechnik GmbH

2 2 Products Cuvette –Biotest Hemoglobin Measuring device –Biotest Hemoglobin Tester

3 3 Function Cuvette Blood capillary, reagent and measuring cuvette in one. Biotest Hemoglobin Tester Photometer with integrated control and digital display

4 4 Function - Cuvette Chemical reaction Free Haemoglobin Sodium nitriteSodium azid Erythrocytes Haemolysis by sodium desoxycholat Methemo- globin Azid- Methaemo -globin

5 5 Function - Cuvette Absorbance curve

6 6 Measurements

7 7 Linearity and precision, n = 20 measurements by each blood sample. Mean value HiCN, i g/dl Biotest Hemoglobin System, g/dl

8 8 Measurements

9 9 Function - Tester Photometer; two wave lengths Evaluation and calculation Reading Evaluation

10 10 Self calibration 100% green light, 100% red light and dark current Compared to moment of calibration If necessary automatic adjustment of LED current Evaluation

11 11 Absorption 256 g/L 12 bits = 4000 steps Resolution 0 = 100 % light 470 – 880 nm Resolution Max deviation = +/- 0,75 % or +/- 30 steps

12 12 Calibration Absorption256 g/L 12 bits = 4000 steps 0 = 100 % light 470 – 880 nm Two points of calibration for linearity; 80 and 220 g/L Three control levels for linearity 120, 150, 180 g/L

13 13 Function - Tester Calculation of Hb Extinction Wave length [nm] Turbidity

14 14 Hb Time 0 min1 min2 min3 min Capillary blood

15 15 Results - Introduction Accuracy –How close to true value is the result Precision –How close to each other are repeated measurements with the same sample

16 16 Results - Introduction Accuracy and precision High accuracy Low precision Low accuracy High precision High accuracy High precision

17 17 Results - Introduction Accuracy: –Deviation (mean) in g/L –Preferred; deviation in % Precision: –Standard deviation SD –Preferred; Coefficient of variation, CV %

18 18 Results Precision with control blood (n=50) ,112,212,312,412,512,612,7 Result [g/dl] Numbers Mean value12,39g/dl SD 0,084g/dl CV0,68%

19 19 Features Cuvette –Aimed for Hb-measurement with Biotest-Hemoglobin-Tester and compatible instruments –High accuracy and precision –Can be used for venous, capillary and arterial blood –Developed to be price worth due to latest production and control technique

20 20 Features Tester Automatic calibration: no calibration or test cuvette needed Cuvette holder minimizing the risk of contamination Automatic monitoring of power supply with fast loading of batteries (1 hour loading > 40 hours of measuring) Automatic shut off with latest value saved Measures only cuvettes with properly haemolysed blood

21 21 Summary High accuracy and precision Easy and reliable system Long time use on battery power

22 22 Results - Introduction


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