Download presentation

Presentation is loading. Please wait.

1
**Biotest Medizintechnik GmbH**

Hemoglobin determination Biotest Hemoglobin System Accuracy and precision Biotest Medizintechnik GmbH

2
**Products Cuvette Measuring device Biotest Hemoglobin**

Biotest Hemoglobin Tester

3
**Function Cuvette Biotest Hemoglobin Tester**

Blood capillary, reagent and measuring cuvette in one. Biotest Hemoglobin Tester Photometer with integrated control and digital display

4
**Function - Cuvette Chemical reaction Haemolysis by sodium desoxycholat**

Sodium nitrite Sodium azid Methemo-globin Free Haemoglobin Azid- Methaemo-globin Erythrocytes

5
Function - Cuvette Absorbance curve

6
Measurements

7
Measurements Linearity and precision, n = 20 measurements by each blood sample. y = 1,0064x - 0,0773 R² = 0,9992 5,00 10,00 15,00 20,00 5,0 g/dl 10,0 g/dl 15,0 g/dl 20,0 g/dl Biotest Hemoglobin System, g/dl Mean value HiCN, i g/dl

8
Measurements

9
**Function - Tester Photometer; two wave lengths**

Evaluation and calculation Reading Evaluation

10
**Self calibration 100% green light, 100% red light and dark current**

Compared to moment of calibration If necessary automatic adjustment of LED current Evaluation

11
**Resolution Max deviation = +/- 0,75 % or +/- 30 steps Absorption**

256 g/L 0 = 100 % light 470 – 880 nm 12 bits = 4000 ”steps” Resolution

12
**Calibration Absorption 256 g/L**

Two points of calibration for linearity; 80 and 220 g/L Three control levels for linearity 120, 150, 180 g/L 0 = 100 % light 470 – 880 nm 12 bits = 4000 ”steps”

13
**Function - Tester Calculation of Hb Extinction Turbidity Turbidity**

Wave length [nm]

14
Capillary blood Hb Time 0 min 1 min 2 min 3 min

15
**Results - Introduction**

Accuracy How close to true value is the result Precision How close to each other are repeated measurements with the same sample

16
**Results - Introduction**

Accuracy and precision High accuracy Low precision Low accuracy High precision High accuracy High precision

17
**Results - Introduction**

Accuracy: Deviation (mean) in g/L Preferred; deviation in % Precision: Standard deviation SD Preferred; Coefficient of variation, CV %

18
**Results Precision with control blood (n=50) Mean value 12,39 g/dl**

30 Mean value 12,39 g/dl SD 0,084 g/dl CV 0,68 % 25 20 Numbers 15 10 5 12,1 12,2 12,3 12,4 12,5 12,6 12,7 Result [g/dl]

19
Features Cuvette Aimed for Hb-measurement with Biotest-Hemoglobin-Tester and compatible instruments High accuracy and precision Can be used for venous, capillary and arterial blood Developed to be price worth due to latest production and control technique

20
Features Tester Automatic calibration: no calibration or test cuvette needed Cuvette holder minimizing the risk of contamination Automatic monitoring of power supply with fast loading of batteries (1 hour loading > 40 hours of measuring) Automatic shut off with latest value saved Measures only cuvettes with properly haemolysed blood

21
**Summary High accuracy and precision Easy and reliable system**

Long time use on battery power

22
**Results - Introduction**

Similar presentations

© 2017 SlidePlayer.com Inc.

All rights reserved.

Ads by Google

Ppt on mars one application Run ppt on website Powerpoint ppt on patient safety goals Ppt on history of capital market in indian Ppt on central administrative tribunal bench What does appt only meant Ppt on solar energy generation Ppt on statistics and probability worksheets Ppt on life study of mathematician salary Ppt on eye oscillation