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Lysmikroskopi MENA3100,OBK, 01.02.11. Lysmikroskop Vi ønsker å se flere detaljer Forstørrelse (magnification) Oppløsningsevne (resolution) Kontrast Optisk.

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Presentation on theme: "Lysmikroskopi MENA3100,OBK, 01.02.11. Lysmikroskop Vi ønsker å se flere detaljer Forstørrelse (magnification) Oppløsningsevne (resolution) Kontrast Optisk."— Presentation transcript:

1 Lysmikroskopi MENA3100,OBK,

2 Lysmikroskop Vi ønsker å se flere detaljer Forstørrelse (magnification) Oppløsningsevne (resolution) Kontrast Optisk mikroskop

3 Simple microscope Lupe, forstørrelsesglass

4 The simple microscope Antony van Leeuwenhoek (1632−1723)

5 The compound microscope ca Hans Janssen Robert Hooke (1635−1703) Micrographia, 1664 Det sammensatte mikroskop

6 Don’t get too hang up in the ray tracing

7 Stereo microscope Stereomikroskop

8 Compound microscope for transmitted light Mikroskop for gjennomfallende lys

9 Inverted compound microscope for reflected light Mikroskop for reflektert lys

10

11

12 jwiley_microscopy/2006_Sept_Hammond.pdf

13 Illuminating system Specimen Filament Field diaphragm Aperture diaphragm Condenser lens

14 Illuminating system Specimen Filament Field diaphragm Aperture diaphragm Condenser lens Köhler illumination Uniform specimen illumination Field diaphragm Controll illuminated area Aperture diaphragm Controll angle of the cone of light

15 Imaging system Specimen Objective Tube lens Eyepiece Okular

16 Primary image

17 Refraction

18 Kromatisk abberation

19 Spherical abberation

20 CorrectionsThe objective

21 The limiting factor: Diffraction

22 R = 0,61 /NA Resolution Rayleigh criterion (Raleigh in Brandon and Kaplan)

23

24 Abbe

25 Numerical aperture (NA) NA = nsin  R = 0,61 /NA Resolution

26 Oil immersion

27 Fouriertransformasjon

28 Condenser system Eyepice Ocular (okular) More is needed than just the objective

29

30 Optical anisotropy Calcite

31 Amorphous and cubic: Optically isotropic, one refracive index Hexagonal, trigonal, tetragonal: Optically anisotropic, two refractive indices Lower symmetry: Optically anisotropic, three refractive indices

32 Optically active crystals in the microscope

33

34 Michel-Levy chart

35

36 Remember that geologists often call their book on microscopy on thin sections for optical crystallography, and microscopy with reflected light is ore microscopy (malmmikroskopi). Some web sites: maintenance:


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