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The identification proteins using mass spectrometry “mass-mapping / peptide mass-fingerprinting” Dr Kevin Mills Institute of Child Health, UCL, London.

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Presentation on theme: "The identification proteins using mass spectrometry “mass-mapping / peptide mass-fingerprinting” Dr Kevin Mills Institute of Child Health, UCL, London."— Presentation transcript:

1 The identification proteins using mass spectrometry “mass-mapping / peptide mass-fingerprinting” Dr Kevin Mills Institute of Child Health, UCL, London

2 The identification proteins using mass spectrometry - amino acid protein Dr Kevin Mills Institute of Child Health, UCL, London

3 The identification proteins using mass spectrometry - arginine protein - lysine Dr Kevin Mills Institute of Child Health, UCL, London

4 The identification proteins using mass spectrometry - arginine - lysine Trypsin Recognises and cleaves the protein at the carboxylic acid of arginine and lysine amino acids Dr Kevin Mills Institute of Child Health, UCL, London

5 The identification proteins using mass spectrometry - arginine - lysine Dr Kevin Mills Institute of Child Health, UCL, London

6 The identification proteins using mass spectrometry - arginine - lysine Dr Kevin Mills Institute of Child Health, UCL, London

7 The identification proteins using mass spectrometry Dr Kevin Mills Institute of Child Health, UCL, London

8 The identification proteins using mass spectrometry Dr Kevin Mills Institute of Child Health, UCL, London

9 The identification proteins using mass spectrometry Tryptic digestion of the protein provides peptide fragments of varying length and therefore mass Dr Kevin Mills Institute of Child Health, UCL, London

10 The identification proteins using mass spectrometry Tryptic digestion of the protein provides peptide fragments of varying length and therefore mass Mass spectrometer Increasing Mass  (longer the peptide the heavier its mass) Dr Kevin Mills Institute of Child Health, UCL, London

11 The identification proteins using mass spectrometry Tryptic digestion of the protein provides peptide fragments of varying length and therefore mass Mass spectrometer Increasing Mass  (longer the peptide the heavier its mass) Dr Kevin Mills Institute of Child Health, UCL, London

12 The identification proteins using mass spectrometry Tryptic digestion of the protein provides peptide fragments of varying length and therefore mass Mass spectrometer Increasing Mass  (longer the peptide the heavier its mass) Dr Kevin Mills Institute of Child Health, UCL, London

13 The identification proteins using mass spectrometry Tryptic digestion of the protein provides peptide fragments of varying length and therefore mass Mass spectrometer Increasing Mass  (longer the peptide the heavier its mass) Dr Kevin Mills Institute of Child Health, UCL, London

14 The identification proteins using mass spectrometry Tryptic digestion of the protein provides peptide fragments of varying length and therefore mass Mass spectrometer Increasing Mass  (longer the peptide the heavier its mass) Dr Kevin Mills Institute of Child Health, UCL, London

15 The identification proteins using mass spectrometry Tryptic digestion of the protein provides peptide fragments of varying length and therefore mass Mass spectrometer Increasing Mass  (longer the peptide the heavier its mass) Dr Kevin Mills Institute of Child Health, UCL, London

16 The identification proteins using mass spectrometry Tryptic digestion of the protein provides peptide fragments of varying length and therefore mass Mass spectrometer Increasing Mass  (longer the peptide the heavier its mass) Dr Kevin Mills Institute of Child Health, UCL, London

17 The identification proteins using mass spectrometry Tryptic digestion of the protein provides peptide fragments of varying length and therefore mass Mass spectrometer Increasing Mass  (longer the peptide the heavier its mass) Dr Kevin Mills Institute of Child Health, UCL, London

18 Increasing Mass  (longer the peptide the heavier its mass) The identification proteins using mass spectrometry Tryptic digestion of the protein provides peptide fragments of varying length and therefore mass Mass spectrometer Dr Kevin Mills Institute of Child Health, UCL, London

19 The identification proteins using mass spectrometry m/z % Increasing peptide length results in increasing mass of the peptide Dr Kevin Mills Institute of Child Health, UCL, London

20 The identification proteins using mass spectrometry Trypsin fragment Dr Kevin Mills Institute of Child Health, UCL, London

21  1 antitrypsin deficiency control plasma Identification of Proteins by Peptide Mass Mapping 3D Model of  1 -antitrypsin In-gel Tryptic digestion Comparison of peptide masses with in silico digestion in data base to obtain “match” Dr Kevin Mills Institute of Child Health, UCL, London

22 Content on this website is the copyright of the Great Ormond Street Hospital for Children and the Institute of Child Health unless otherwise stated. Where copyright applies, visitors can download material for private research, study or in-house use only. Visitors must not copy, distribute, or publish any material from this website. Any other use of copyright material requires the formal permission of the Great Ormond Street Hospital for Children and the Institute of Child Health. Information specifically for patients and families has been reviewed for accuracy, but we hold no responsibility for alterations made thereafter without our consent. The content of this presentation is the copyright of Dr Kevin Mills, UCL Institute of Child Health. Visitors must not copy, distribute, or publish any material from this presentation. It is strictly forbidden to alter this presentation in any way. Any other use of this presentation requires formal permission from Dr Mills. (c) Dr Kevin Mills, UCL Institute of Child Health, London, 2006.


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