Presentation on theme: "Mass Spectrometry Week 2 Lab S117 Fall 2011. Overview Post-Lab: Percent Alcohol in Wine – TurnItIn.com – Working with data sets – Analyzing data sets."— Presentation transcript:
Overview Post-Lab: Percent Alcohol in Wine – TurnItIn.com – Working with data sets – Analyzing data sets – Analysis of experimental procedure Pre-lab: Mass Spectrometry – Fragmentation – Protein Analysis Practical matters of lab meeting
Assignments Sign up for turnitin.com Formal reports due electronically up to time of lab One minute 24 hours late = 20pt penalty Give copy of lab notebook to AI in lab
Excel Working with data sets Statistical Analysis Certainty and uncertainty – Measurements – Experimental limit – Reproducibility
Experimental Design Practical Weaknesses? Propagation of Error Was the distillation useful?
Pre-Lab: Mass Spectrometry Analysis of isotopes!
Nominal Mass and Exact Mass Nominal Mass: Low resolution – 35 Cl = 35 Da – 37 Cl = 37 Da – CO = N 2 = 28 Da Exact Mass: High Resolution – CO = 27.9949 Da – N 2 = 28.0061 Da
Analysis of Molecules Parent peak (molecular ion)and fragment peaks Analysis of fragment patterns useful Application to Cl 2 caffeine isovaleraldehyde
Analysis of Biomolecules Complex molecules Molecular mass in kiloDaltons (kDa) Nobel Prizes for MS of proteins