1. Immunoblot and ChIP analyses showing that AhRR was not involved in the lack of ABCG2 upregulation in SW620 cells treated with romidepsin or B(a)P.
Immunoblot and ChIP analyses showing that AhRR was not involved in the lack of ABCG2 upregulation in SW620 cells treated with romidepsin or B(a)P. A, immunoblot analysis showing the cellular level of AhRR in various cell lines indicated after treatment of romidepsin or B(a)P. HepG2 and HeLa cells were known to express detectable protein level of AhRR, therefore they were included for comparison. GAPDH was used as a loading control. No activation of AhRR was detected in all the cell lines tested. B, ChIP analyses targeting the proximal and distal region of the ABCG2 promoter in romidepsin- or B(a)P-treated SW620 cells, using anti-AhRR antibody. Input, DNA isolated from the lysate before immunoprecipitation (only 10% of the total chromatin was used for the PCR reactions and it was run as the “input” in the gel electrophoresis); IgG, ChIP using normal IgG for immunoprecipitation. A representative gel image from 2 independent immunoprecipitations is shown.
Kenneth K.W. To et al. Mol Cancer Res 2011;9:
©2011 by American Association for Cancer Research