1. Expression changes of specific epigenetic-controlled tumor-related genes by the prenatal/maternal BSp treatment. qRT-PCR and Western blot analysis were performed to measure gene expression of tumor suppressor genes such as p53 and p16 (A and C), and tumor-p...
Expression changes of specific epigenetic-controlled tumor-related genes by the prenatal/maternal BSp treatment. qRT-PCR and Western blot analysis were performed to measure gene expression of tumor suppressor genes such as p53 and p16 (A and C), and tumor-promoting genes such as TERT and c-Myc (B and D) in breast tumors of SV40 mouse offspring born to the mothers treated with the BSp diet. A and B, Relative gene transcription expression. C and D, Western gel blot showing protein levels. β-Actin antibody was used to ensure equal loading. Representative photograph from an experiment was repeated three times. E and F, Histogram of quantification of the protein levels. Data are in triplicate from three independent experiments and were normalized to GAPDH or β-actin and calibrated to levels in untreated samples. Columns, mean; bars, SD; *, P < 0.05; **, P < 0.01; ***, P < 0.001, significantly different from control.
Yuanyuan Li et al. Cancer Prev Res 2018;11:
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