1. PTB-independent ShcA pools require Src to promote mammary tumorigenesis.
PTB-independent ShcA pools require Src to promote mammary tumorigenesis. A, Src was deleted from ErbB2 mammary epithelial cell line (NMuMG-NeuNT) from the indicated ShcA-expressing cell lines using Crispr/Cas9 genomic editing. Immunoblot analysis of vector control and Src-Crispr (CR) cell lines using Src- and Tubulin-specific antibodies. Vector control and Src-CR of NMuMG-NeuNT ShcAWT (B) and PTBMUT (C) cell lines were injected into the mammary fat pads of immunodeficient mice. The data depict the average treumor volumes (mm3) ± SEM and are representative of 9–10 tumors per group. *, P < 0.05; **, P < 0.01; ****, P < IHC staining of pY416-Src (D) and pS240/244-rS6 (E) in ShcAWTand PTBMUT-expressing NMuMG-NeuNT mammary tumors. The data depict the average positively stained cells or pixels ± SEM is representative of 8–10 tumors per group. **, P < F, FLAG immunoprecipitates from indicated cell lines were probed with pY239/240-ShcA or ShcA-specific antibodies via immunoblot analysis. The graph represents densitometric quantification of three independent experiments using ImageJ software. **, P < G, Clonogenic assay of specified cell lines treated with DMSO, lapatinib (0.5 μmol/L), PP2 (2 μmol/L) alone, or in combination. The data is shown as fold change in viability relative to DMSO control and is representative of three independent experiments (means ± SEM). *, P < 0.05; ***, P <
Jacqueline R. Ha et al. Mol Cancer Res 2018;16:
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