1. ChREBPα increases β-cell mitochondrial activity and ATP production in human islets.
ChREBPα increases β-cell mitochondrial activity and ATP production in human islets. A: Dispersed human islets were transduced with the indicated adenovirus, treated as shown, and labeled with MitoTracker Red CMXRos (Mito) and an anti-insulin antibody (ins). Scale bars = 20 μm. B: The mitochondrial signal intensity was determined from the images in A and is shown relative to the control (treated with low glucose [Glu]). C: Total intracellular ATP content was determined in human islets and normalized to protein content. Data are shown as a percentage of the control (stimulated with low Glu and treated with Ad.LacZ). The values shown are the mean ± SE (n = 3–5). *P < 0.05, **P < MFI, mean fluorescence intensity.
Anil Kumar et al. Diabetes 2018;67:
©2018 by American Diabetes Association