1. KG-501 suppresses NSCLC/IL-1β CM–induced migration of HUVECs by regulating IL-1β–induced CXC chemokine gene expression in NSCLC cells.
KG-501 suppresses NSCLC/IL-1β CM–induced migration of HUVECs by regulating IL-1β–induced CXC chemokine gene expression in NSCLC cells. A, HUVEC migration was induced with CM from A549 cells treated with control medium (a) or with medium containing 2.5 ng/mL IL-1β (b), 10 μmol/L KG-501 (c), 2.5 ng/mL IL-1β plus 2.5 μmol/L KG-501 (d), 2.5 ng/mL IL-1β plus 5 μmol/L KG-501 (e), or 2.5 ng/mL IL-1β plus 10 μmol/L KG-501 (f) in serum-free medium for 24 h. B, quantification of image analysis for the HUVEC migration assay. C, total RNAs were isolated from cells with the treatment described in A, and real-time PCR was done to measure the expression level of the indicated genes. D, the concentration of CXCL5 and CXCL8 protein in the CM was measured with ELISA. Results were normalized according to the cell number. Columns, mean; bars, SE. **, P < 0.01; ***, P <
Hongxia Sun et al. Cancer Prev Res 2008;1:
©2008 by American Association for Cancer Research