Presentation on theme: "New Technologies and Innovations in Laboratory Support of HIV/AIDS care and Treatment Dr. Thaweesak Tirawatnapong Chula Medical Research Center (Chula."— Presentation transcript:
New Technologies and Innovations in Laboratory Support of HIV/AIDS care and Treatment Dr. Thaweesak Tirawatnapong Chula Medical Research Center (Chula MRC)
Diagnostics Tests for HIV 1 Tests to facilitate initial diagnosis 2 Tests to stage the patients 3 Tests to monitor the patients
Important of HIV Testing Known HIV status for care and treatment For prevention measure ProblemProblem Poor HIV testing coverage in resource- limited Setting
General Community Requirement Need to increase the level of access to high quality diagnostic in resource-limited settings To facilitate early detection and treatment of HIV/AIDS Simplify and improve the efficiency of HIV testing Tests design to be delivered at The point of care (POC) or Near POC
1. Sample preparation, including viral concentration and nucleic acid extraction. 2. Amplification of the target DNA or RNA. 3. Detection of the amplified product. The Various PCR Process have Three Steps in Common
New Technologies for Viral Load Testing Alere NAT system disposable cartridge that provides for sample collection, cell lysis, specific target capture, reverse transcription, polymerase chain reaction amplification and real time fluorescence detection based on reporter probe hybridization on an integrated micro probe array.
The Liat Analyzer is developed for nucleic acid based testing and the Liat Tubes serve both as collection devices and as test chambers, maintaining samples in a closed system from sample collection through sample disposal. We believe that the Liat system is suitable for a wide range of testing labs and near-patient test settings due to its ease of use, fast turnaround time, cost effectiveness and safety features.
NanoStrings Technology employs two ~50 base probes per mRNA that hybridize in solution. The Reporter Probe carries the signal; the Capture Probe allows the complex to be immobilized for data collection Hybridization
After hybridization, the excess probes are removed and the probe/target complexes aligned and immobilized in the nCounter Cartridge. Purification and Immobilization
Data Collection Sample Cartridges are placed in the Digital Analyzer for data collection. Color codes on the surface of the cartridge are counted and tabulated for each target molecule
nCounter Analysis System High level of precision High level of sensitivity (<1 copy per cell) No enzyme processing or Amplification Quantification
Multiplexed Diagnostic LLNL Awarded $2.5M to Move Pathogen Detection Tests to NanoString Platform June 07, 2011 The National Institutes of Health has awarded $2.5 million to researchers at Lawrence Livermore National Laboratory to support the development of pathogen detection assays on NanoString Technologies' nCounter system. The project aims to develop high-content, multiplex assays capable of simultaneously detecting 35 National Institute of Allergy and Infectious Diseases category A, B, and C viral agents, plus quantifying cytokine and chemokine responses in patients,