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Antisense basic fibroblast growth factor gene transfer reduces neointimal thickening after arterial injury  Abigail K. Hanna, MD, Jonathan C. Fox, MD,

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Presentation on theme: "Antisense basic fibroblast growth factor gene transfer reduces neointimal thickening after arterial injury  Abigail K. Hanna, MD, Jonathan C. Fox, MD,"— Presentation transcript:

1 Antisense basic fibroblast growth factor gene transfer reduces neointimal thickening after arterial injury  Abigail K. Hanna, MD, Jonathan C. Fox, MD, PhD, David G. Neschis, MD, Shawn D. Safford, BS, Judith L. Swain, MD, Michael A. Golden, MD  Journal of Vascular Surgery  Volume 25, Issue 2, Pages (February 1997) DOI: /S (97) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Composite of photomicrographs of representative cross-sections from arteries treated with either control adenovirus transporting Ad.lacZ, the same adenovirus transporting Ad.ASbFGF, or vehicle alone (PBS). Control arteries are in top row (A-C); bottom row contains images of arteries treated with increasing doses of Ad.ASbFGF (left to right). In each micrograph, the neointima is demarcated between two black arrows. Treatment with the lowest dose of Ad.ASbFGF, 2 × 109 pfu/ml (D), does not significantly decrease neointimal thickening compared with control arteries (top row). Ad.ASbFGF at a slightly higher dose, 1 × 1010 pfu/ml (E), results in a reduction of the intimal thickening response by 66%. Highest dose of Ad.ASbFGF, 1 × 1011 pfu/ml (F), shows nearly negligible intimal thickening. The arteries depicted in images C and G were treated with the same concentration of adenovirus, 1 × 1010 pfu/ml, but C was treated with the Ad.lacZ gene, and G was treated with the Ad.ASbFGF gene. These images, taken at lower magnification, demonstrate that response to Ad.ASbFGF (a 66% reduction in neointimal hyperplasia) is uniform along lumen circumference (G). All tissues are stained with hematoxylin-eosin except for images A and F, which are prepared with elastin-collagen stain to demonstrate the elastin layers of the media. Images A, B, D, E, and F are magnified by 125X. Images C and G are magnified by 50X. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 1 Composite of photomicrographs of representative cross-sections from arteries treated with either control adenovirus transporting Ad.lacZ, the same adenovirus transporting Ad.ASbFGF, or vehicle alone (PBS). Control arteries are in top row (A-C); bottom row contains images of arteries treated with increasing doses of Ad.ASbFGF (left to right). In each micrograph, the neointima is demarcated between two black arrows. Treatment with the lowest dose of Ad.ASbFGF, 2 × 109 pfu/ml (D), does not significantly decrease neointimal thickening compared with control arteries (top row). Ad.ASbFGF at a slightly higher dose, 1 × 1010 pfu/ml (E), results in a reduction of the intimal thickening response by 66%. Highest dose of Ad.ASbFGF, 1 × 1011 pfu/ml (F), shows nearly negligible intimal thickening. The arteries depicted in images C and G were treated with the same concentration of adenovirus, 1 × 1010 pfu/ml, but C was treated with the Ad.lacZ gene, and G was treated with the Ad.ASbFGF gene. These images, taken at lower magnification, demonstrate that response to Ad.ASbFGF (a 66% reduction in neointimal hyperplasia) is uniform along lumen circumference (G). All tissues are stained with hematoxylin-eosin except for images A and F, which are prepared with elastin-collagen stain to demonstrate the elastin layers of the media. Images A, B, D, E, and F are magnified by 125X. Images C and G are magnified by 50X. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 1 Composite of photomicrographs of representative cross-sections from arteries treated with either control adenovirus transporting Ad.lacZ, the same adenovirus transporting Ad.ASbFGF, or vehicle alone (PBS). Control arteries are in top row (A-C); bottom row contains images of arteries treated with increasing doses of Ad.ASbFGF (left to right). In each micrograph, the neointima is demarcated between two black arrows. Treatment with the lowest dose of Ad.ASbFGF, 2 × 109 pfu/ml (D), does not significantly decrease neointimal thickening compared with control arteries (top row). Ad.ASbFGF at a slightly higher dose, 1 × 1010 pfu/ml (E), results in a reduction of the intimal thickening response by 66%. Highest dose of Ad.ASbFGF, 1 × 1011 pfu/ml (F), shows nearly negligible intimal thickening. The arteries depicted in images C and G were treated with the same concentration of adenovirus, 1 × 1010 pfu/ml, but C was treated with the Ad.lacZ gene, and G was treated with the Ad.ASbFGF gene. These images, taken at lower magnification, demonstrate that response to Ad.ASbFGF (a 66% reduction in neointimal hyperplasia) is uniform along lumen circumference (G). All tissues are stained with hematoxylin-eosin except for images A and F, which are prepared with elastin-collagen stain to demonstrate the elastin layers of the media. Images A, B, D, E, and F are magnified by 125X. Images C and G are magnified by 50X. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 1 Composite of photomicrographs of representative cross-sections from arteries treated with either control adenovirus transporting Ad.lacZ, the same adenovirus transporting Ad.ASbFGF, or vehicle alone (PBS). Control arteries are in top row (A-C); bottom row contains images of arteries treated with increasing doses of Ad.ASbFGF (left to right). In each micrograph, the neointima is demarcated between two black arrows. Treatment with the lowest dose of Ad.ASbFGF, 2 × 109 pfu/ml (D), does not significantly decrease neointimal thickening compared with control arteries (top row). Ad.ASbFGF at a slightly higher dose, 1 × 1010 pfu/ml (E), results in a reduction of the intimal thickening response by 66%. Highest dose of Ad.ASbFGF, 1 × 1011 pfu/ml (F), shows nearly negligible intimal thickening. The arteries depicted in images C and G were treated with the same concentration of adenovirus, 1 × 1010 pfu/ml, but C was treated with the Ad.lacZ gene, and G was treated with the Ad.ASbFGF gene. These images, taken at lower magnification, demonstrate that response to Ad.ASbFGF (a 66% reduction in neointimal hyperplasia) is uniform along lumen circumference (G). All tissues are stained with hematoxylin-eosin except for images A and F, which are prepared with elastin-collagen stain to demonstrate the elastin layers of the media. Images A, B, D, E, and F are magnified by 125X. Images C and G are magnified by 50X. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 1 Composite of photomicrographs of representative cross-sections from arteries treated with either control adenovirus transporting Ad.lacZ, the same adenovirus transporting Ad.ASbFGF, or vehicle alone (PBS). Control arteries are in top row (A-C); bottom row contains images of arteries treated with increasing doses of Ad.ASbFGF (left to right). In each micrograph, the neointima is demarcated between two black arrows. Treatment with the lowest dose of Ad.ASbFGF, 2 × 109 pfu/ml (D), does not significantly decrease neointimal thickening compared with control arteries (top row). Ad.ASbFGF at a slightly higher dose, 1 × 1010 pfu/ml (E), results in a reduction of the intimal thickening response by 66%. Highest dose of Ad.ASbFGF, 1 × 1011 pfu/ml (F), shows nearly negligible intimal thickening. The arteries depicted in images C and G were treated with the same concentration of adenovirus, 1 × 1010 pfu/ml, but C was treated with the Ad.lacZ gene, and G was treated with the Ad.ASbFGF gene. These images, taken at lower magnification, demonstrate that response to Ad.ASbFGF (a 66% reduction in neointimal hyperplasia) is uniform along lumen circumference (G). All tissues are stained with hematoxylin-eosin except for images A and F, which are prepared with elastin-collagen stain to demonstrate the elastin layers of the media. Images A, B, D, E, and F are magnified by 125X. Images C and G are magnified by 50X. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7 Fig. 1 Composite of photomicrographs of representative cross-sections from arteries treated with either control adenovirus transporting Ad.lacZ, the same adenovirus transporting Ad.ASbFGF, or vehicle alone (PBS). Control arteries are in top row (A-C); bottom row contains images of arteries treated with increasing doses of Ad.ASbFGF (left to right). In each micrograph, the neointima is demarcated between two black arrows. Treatment with the lowest dose of Ad.ASbFGF, 2 × 109 pfu/ml (D), does not significantly decrease neointimal thickening compared with control arteries (top row). Ad.ASbFGF at a slightly higher dose, 1 × 1010 pfu/ml (E), results in a reduction of the intimal thickening response by 66%. Highest dose of Ad.ASbFGF, 1 × 1011 pfu/ml (F), shows nearly negligible intimal thickening. The arteries depicted in images C and G were treated with the same concentration of adenovirus, 1 × 1010 pfu/ml, but C was treated with the Ad.lacZ gene, and G was treated with the Ad.ASbFGF gene. These images, taken at lower magnification, demonstrate that response to Ad.ASbFGF (a 66% reduction in neointimal hyperplasia) is uniform along lumen circumference (G). All tissues are stained with hematoxylin-eosin except for images A and F, which are prepared with elastin-collagen stain to demonstrate the elastin layers of the media. Images A, B, D, E, and F are magnified by 125X. Images C and G are magnified by 50X. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

8 Fig. 1 Composite of photomicrographs of representative cross-sections from arteries treated with either control adenovirus transporting Ad.lacZ, the same adenovirus transporting Ad.ASbFGF, or vehicle alone (PBS). Control arteries are in top row (A-C); bottom row contains images of arteries treated with increasing doses of Ad.ASbFGF (left to right). In each micrograph, the neointima is demarcated between two black arrows. Treatment with the lowest dose of Ad.ASbFGF, 2 × 109 pfu/ml (D), does not significantly decrease neointimal thickening compared with control arteries (top row). Ad.ASbFGF at a slightly higher dose, 1 × 1010 pfu/ml (E), results in a reduction of the intimal thickening response by 66%. Highest dose of Ad.ASbFGF, 1 × 1011 pfu/ml (F), shows nearly negligible intimal thickening. The arteries depicted in images C and G were treated with the same concentration of adenovirus, 1 × 1010 pfu/ml, but C was treated with the Ad.lacZ gene, and G was treated with the Ad.ASbFGF gene. These images, taken at lower magnification, demonstrate that response to Ad.ASbFGF (a 66% reduction in neointimal hyperplasia) is uniform along lumen circumference (G). All tissues are stained with hematoxylin-eosin except for images A and F, which are prepared with elastin-collagen stain to demonstrate the elastin layers of the media. Images A, B, D, E, and F are magnified by 125X. Images C and G are magnified by 50X. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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