1. Bringing critical systems thinking to high school students through ocean acidification research An overview for high school teachers

2. Program Vision To teach science as an engaging interdisciplinary subject using research-based education and current scientific practices Specifically – We help students develop the thinking and concepts required for systems science by using a common problem that inherently brings together biology, chemistry, physics, mathematics, statistics and computer science

3. Program Mission: Cultivate cross-disciplinary skills for solving complex problems

4. Why and how we bring Ocean Acidification research to 6-12 th graders…

5. Crucial for today's students to fully understand –scientific problem with largely anthropogenic roots with serious biological and societal consequences Inquiry based learning and experimentation that closely models what is occurring in laboratories across the world. Students can act as both scientists and delegates Interdisciplinary Addresses the Next Generation Science Standards The Changing Carbon Cycle: A Scientific and Societal Problem

6. Common problem/story is ocean acidification http://www.esrl.noaa.gov/gmd/ccgg/trends/history.html Each of our modules has an inherently interdisciplinary, systems problem or situation that allows students to learn about the process of research. For this module, ocean acidification is that situation. For your background information, the most important thing to realize is that the rate of change of CO 2 in our atmosphere is 10-100 times greater than ever observed in our Earths history. What does this mean for the 70% of our planet that is our ocean and for all of us who are connected to this immensely important system?

7. Ocean Acidification: A Systems Approach to a Global Problem A curriculum unit that models current research and connects to the work of others Students act as scientists and delegates. 3-5 weeks of class time. Photos: genome.jgi-psf.org/Thaps3, NASA, www.pnas.org/content/105/5/1391/F1.expansion.htmlgenome.jgi-psf.org/Thaps3 www.pnas.org/content/105/5/1391/F1.expansion.html

8. Broad Curriculum Module Goal: Analyze the effect increasing atmospheric CO 2 has on ocean chemistry, ecosystems and human societies Prior Knowledge Needed to achieve this goal: Understand the basics of networks. Lesson 1 and 2 of our Ecological Networks module can help with this understanding: http://baliga.systemsbiology.net/drupal/education/?q=content/ec ological-networks-course

9. EcoNet Lesson 1 - Classroom exercise: analyzing a social network 1.In an interactive group activity, students use familiar cell phone networks to learn about how information can be easily depicted. 2. Students pull together the class information to quickly learn that even when working in a team of five, it is still difficult to organize and analyze all of the information.

10. EcoNet Lesson 2: Motivation to use tools to solve problems

11. We know that systems thinking enables behavioral change. The cell phone and cytoscape lessons help build systems thinking skills and can be applied in many contexts throughout the year. When teachers complete the OA lessons with these two prior lessons, students learning is greatly enhanced for each of the following lessons. Poster: Waters Foundation

12. To introduce the module – there are many possibilities The news pieces in Lesson 1 serve as a great intro. Students see they are studying a problem concurrently with top scientists. We all need to decipher the evidence in order to understand and make decisions about what actions to take. Or you can give them a scenario that they will follow through and build on subsequent lessons: You are a different person or organism. As a person, your finances have drastically changed (some for the better, some NOT for the better). If you are an organism: you have noticed a change – youre not sure what is happening but you have noticed small things. For instance, previously you could look in the farmers almanac and predict life for the next few months. You notice your growing season has changed, or things sound or smell different, or things just arent right. Well use interdisciplinary science, collaboration & systems thinking to figure out what is wrong and what we can expect in the future!

13. Lesson 1 (Introduction through case studies): Understand the broad reach and accessibility of ocean studies. Gain the critical thinking skills to properly evaluate news media. Students each read a news article and critically assess its validity using SAT reading techniques. Each student then contributes their articles key nodes to help create an interconnected diagram. As a class they identify important points or nodes that they want to study in their setting.

14. Round Robin Labs: Respiration, Combustion, Reactions, Detecting Lesson 2: Exploring CO 2 in the lab A.Use inquiry to understand CO 2. B.Learn the basics of the changing carbon cycle and ocean acidification. Lesson 1Lesson 2 Option 1 Option 2 Open Inquiry

15. Lesson 3 Watch Acid Test video – realize this is a global problem with many stakeholders Setting the stage to model a collaborative lab group Is this a situation that requires a systems study? –Many parts with interactions, emergent properties, reverberating effects?

16. Does this require a systems study? Trees and Plants (photosynthesis) Animals (respiration) Phytoplankton (photosynthesis) Marine Animals (respiration) ? Combustion Reactions

17. Balancing versus reinforcing loops Questions for students to explore: Do you think this is a balancing or reinforcing loop? What about the CO 2 loop in our environment? How can we learn more?

18. By the end of Lesson 3 (see lesson plan for more info) Students will decide which interest group they would like to align themselves with.

19. Delegates to the International Convention on the Impacts of the Changing Carbon Cycle on Ocean Systems. Low CO 2 Emitter Interest Groups High CO 2 Emitter Photosynthesizing organisms Marine calcifying organisms One of each group; duplicates as needed.

20. Lessons 4-5: Exploration of the effects of changing CO 2 and nutrient cycles Main question: What effect does the increasing atmospheric CO 2 have on the ocean and its subsystems? Model collaborative systems research Analyze multiple and diverse data Use multiple stressors

21. Options (while in their interest groups) A.Student groups each design their own experiment B.8 or so protocols available for student groups to complete all with slight variations (see Lesson 4 Teacher Resources for more information) Examples: Diatoms – various nutrient, CO 2 entry, water, temperature, salinity types Shell and bone dissolution with sea urchin online lab –All have some online data component

22. Visible results of trial run conducted by ISB high school interns. Differences in cell density across varying media are visibly detectable. Flasks shown one week after inoculation.

23. Growth curves as determined from hemocytometer counts performed by ISB high school interns

24. Need for multiple & diverse data Daily culture measurements: –Cell count using a hemocytometer –OD 600 reading/Fluorometer reading (depending on what technology is available) –Pigment description –Pigment extraction experiment Chromatography Chromatogram from Henderson State Univ. http://198.16.16.43/content.aspx?id=7261

25. Example of experiment design 5g of dry ice were used to stabilize CO 2 levels at approximately 2000 ppm. pH of seawater dropped from 8.0 to 6.5 overnight. Shells left in seawater lost 2% of their mass over 3 days.

26. NetLogo / Java simulation for generating hypotheses.

27. Supplement their experiment with online data component. Bad Acid: Sea Urchin Simulation C-MORE WA State Department of Ecology (Eyes over Puget Sound) Multiple in situ sensors Ice Core studies Mesocosm studies Many NOAA resources Carbon footprint calculators

28. In addition to completing their own experiment, students will explore an online data component. Some experiments are more technical than others, so the groups with less time intensive experiments may complete the majority of the online portions. Jigsawing the activities is a good option: –Ice Core Exploration –Mesocosms –Eyes over Puget Sound –Students in the Marine Calcifying group should also complete the Online Stanford Sea Urchin – Acid Ocean activity: http://virtualurchin.stanford.edu/AcidOcean/AcidOcean.htm

29. Once students have their data Help them loop back to all they have learned. They should begin stepping back to make a map with connections between all of the interacting parts. Help them understand the subsystem they explored. Then have them step back to connect this to the entire system as they begin to prepare for their summit. The DRAFT figures on the following slides may be used to help them see the positive and negative impact of various parts in this system.

30. Atmospheric CO2 Level Ecosystem Services CO 2 Absorbed by Ocean Nutrients Higher Trophic Level Fish Dependent on Marine Calcifiers Fisheries Reef Tourism CO 2 Polluting Nations Developing Island Nation Economies Marine Calcifying Organisms Diatoms Oxygen Carbonic Acid Calcium Carbonate

31. Atmospheric CO2 Level Ecosystem Services CO 2 Absorbed by Ocean Nutrients Higher Trophic Level Fish Dependent on Marine Calcifiers Fisheries Reef Tourism CO 2 Polluting Nations Developing Island Nation Economies Marine Calcifying Organisms Diatoms Oxygen Carbonic Acid Calcium Carbonate + + ++ + - - - - - - - - - -

32. Diatoms Atmospheric CO2 Level CO2 Absorbed by Ocean Light Temperature NitrogenPhosphorusSilica Winter Storms/Depth of Mixing Oxygen Zooplankton + + + ++ + + -- - - - - -

33. Lesson 6: Mock Summit Research summits, position statements, etc. Each group presents for 2 minutes, others take notes only Questioning protocol

34. Lesson 6 – Mock Summit Each group presents for 2 minutes, others take notes only –Poster, lab report, powerpoint? –What interest group, research report, data collected, experimental data that supports position, future next steps for your research –Recommendations for future research –Policy recommendations for individuals, as a member of the interest group, for all others

35. Delegates reconvene Discussion of findings –Emphasis placed on the impact on the given network. –Recommendations crafted for scientists, politicians and people across the world. –Students reflect on unanswered questions and on what their individual roles in the networks theyve studied are. How they might change their actions in order to impact the network? What does their final, class experimental network look like?

36. Flow of module

37. Please complete both the pre and post assessments. We would appreciate any copies. Hard copies available online Or use our survey monkey –Preassessment: https://www.surveymonkey.com/s/XKSWTML –Postassessment: https://www.surveymonkey.com/s/X8VSZJS Attn: Claudia Ludwig, 401 Terry Avenue N., Seattle, WA 98109

38. Assessment results elucidate emphasis Incorporating interest groups leads to passionate and global perspectives –No interest group = decreased passion and perspective Incorporating systems level emphasis = great gains –No systems emphasis = gain in general scientific thinking without systems. The difference is that students do not gain understanding in coupled factors, need for multiple data types or the importance of modeling if a systems emphasis is not used. If cell phone lessons were completed –Students focus more on reverberating effects and tend to see the big picture. They also articulate these effects and views more fully during the summit when they have completed the cell phone lessons 1 and 2.

39. Anecdotal Student Comments Students are engaged and learn a tremendous amount during this module. Here are a couple of student comments: –This was the most fun I have ever had in a science class. –I wish we had more time for this.

40. Thanks for your interest! Please let us know if you would like to participate more in our program and/or if you have any feedback! From the OA Curriculum team…

41. We thank NSF (OCF 0928561) and NIH/NIGMS for leveraged dissemination. Please see http://baliga.systemsbiology.net/ for more information.http://baliga.systemsbiology.net/