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Novel phosphorylation sites on H+-ATPase proteins

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Presentation on theme: "Novel phosphorylation sites on H+-ATPase proteins"— Presentation transcript:

1 Novel phosphorylation sites on H+-ATPase proteins
Novel phosphorylation sites on H+-ATPase proteins.Top, MS/MS spectra for the phosphopeptides E889AVNIFPEKGpSYR901 of ATPase 2 (At4g30190) and ELpSEIAEQAK (conserved between ATPase 1 (At2g18960) and ATPase 2). Novel phosphorylation sites on H+-ATPase proteins.Top, MS/MS spectra for the phosphopeptides E889AVNIFPEKGpSYR901 of ATPase 2 (At4g30190) and ELpSEIAEQAK (conserved between ATPase 1 (At2g18960) and ATPase 2). Bottom, ClustalW analysis of the C termini of ATPase isoforms 1–11 of Arabidopsis. The identified phosphopeptides are underlined and phosphorylated residues printed in bold. The peptide ELpSEIAEQAK comes from either AHA1 or AHA2; the peptide LKGLDIETIQQAYYpTV from either AHA4 or AHA11. Thomas S. Nühse et al. Mol Cell Proteomics 2003;2: © 2003 The American Society for Biochemistry and Molecular Biology

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binding sites 58 of the 473 unambiguously assigned phosphorylation sites are predicted by Scansite to be sites for binding. 50 of these correspond.
Quantification of C-terminal phosphorylation of AtPIP2;1 upon NaCl (A) and H2O2 (B) treatments.A, plants were either untreated (white bars) or treated.

Quantification of C-terminal phosphorylation of AtPIP2;1 upon NaCl (A) and H2O2 (B) treatments.A, plants were either untreated (white bars) or treated.
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Sequence alignment of C-terminal phosphorylated plant aquaporins
Principle for quantification of phosphorylation of the C-terminal tail of AtPIP2;1. Principle for quantification of phosphorylation of the C-terminal tail.

Principle for quantification of phosphorylation of the C-terminal tail of AtPIP2;1. Principle for quantification of phosphorylation of the C-terminal tail.
MALDI-TOF MS spectrum of phosphopeptides from plant PM aquaporins.

MALDI-TOF MS spectrum of phosphopeptides from plant PM aquaporins.
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Phosphopeptide sequencing by MALDI-TOF/TOF of the C-terminal tail of AtPIP2;1.A, MS/MS spectrum of singly phosphorylated 277SLGSFRSAANV287 (m/z ).
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Distribution of disorder in the cytosolic phosphoproteome
Experimental overview A sequential protein and peptide IMAC enrichment was analyzed by four different LC-MS/MS strategies. Experimental overview A sequential.

Experimental overview A sequential protein and peptide IMAC enrichment was analyzed by four different LC-MS/MS strategies. Experimental overview A sequential.
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Distribution of phosphorylation sites identified in the cytosolic phosphoproteome.A, numbers of approved phosphopeptides, previously phosphorylated peptides,

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Phosphopeptide sequencing by MALDI-TOF/TOF of the C-terminal tail of AtPIP2;7.A, MS/MS spectrum of singly phosphorylated 270ALGSFRSNATN280 (m/z 1217.53).

Phosphopeptide sequencing by MALDI-TOF/TOF of the C-terminal tail of AtPIP2;7.A, MS/MS spectrum of singly phosphorylated 270ALGSFRSNATN280 (m/z ).
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