Presentation on theme: "Design and Calibration of a Dissolution Test Equipment Training Workshop on Dissolution, Pharmaceutical Product Interchangeability and Biopharmaceutical."— Presentation transcript:
1 Design and Calibration of a Dissolution Test Equipment Training Workshop on Dissolution, Pharmaceutical Product Interchangeability and Biopharmaceutical Classification System. Kyiv, Ukraine, JuneDr. Sandra Klein, Institute of Pharmaceutical Technology,Johann Wolfgang Goethe University Frankfurt
2 Dosage forms to be tested immediate release dosage formspowders, granules / beads, tablets, capsulescontrolled release dosage formstransdermal systemsimplants
3 Official Dissolution Monographs United States PharmacopeiaUSP XXX (30)European PharmacopoeiaPh. Eur. 5th Edition, Supplement 5.3British PharmacopoeiaBP 2007Japanese PharmacopoeiaJP XIV (14)
4 Official dissolution apparatus USP 30 classificationRotating Basket (Ph.Eur./BP/JP)Paddle (Ph.Eur./BP/JP)Reciprocating Cylinder (Ph.Eur.)Flow Through Cell (Ph.Eur./BP/JP)Paddle Over Disk (Ph.Eur.)Rotating Cylinder (Ph.Eur.)Reciprocating Holder
5 Which type of dissolution apparatus ? Depends on intentionQuality controlexamining batch homogeneityexamining batch to batch conformityexamining stabilityResearch & Developmentexamining drug release behavior of preformulationsin vitro simulation of the gastrointestinal passageIVIVC
6 Apparatus 1 - Basket Useful for Standard volume capsules beads delayed release / enteric coated dosage formsfloating dosage formssurfactants in mediaStandard volume900/1000 ml1, 2, 4 liter vessels
7 Apparatus 1 - Basket Advantages breadth of experience (more than 200 monographs)full pH change during the testcan be easily automated which is important for routine investigations
8 Apparatus 1 - Basket Disadvantages disintegration-dissolution interactionhydrodynamic „dead zone“ under the basket degassing is particularly importantlimited volume sink conditions for poorly soluble drugs ?
10 Apparatus 2 - Paddle Useful for Standard volume tabletscapsulesbeadsdelayed release / enteric coated dosage formsStandard volume900/1000 mlMethod of first choice !!!
11 Apparatus 2 - Paddle Advantages easy to use robust can be easily adapted to apparatus 5long experiencepH change possiblecan be easily automated which is important for routine investigations
12 Apparatus 2 - Paddle Disadvantages pH/media change is often difficult limited volume sink conditions for poorly soluble drugs ?hydrodynamics are complex, they vary with site of the dosage form in the vessel (sticking,floating) and therefore may significantly affect drug dissolution„coning“sinkers for floating dosage forms
13 Sinker types JP/ USP / Ph. Eur. 5.3 Sinker „a small loose piece of nonreactive material such asnot more than a few turns of wire helix may be attachedto dosage units that would otherwise float …“„…. other validated sinker devices may be used“
16 Apparatus 3 – Reciprocating cylinder Useful fortabletsbeadscontrolled release formulationsStandard volumeml per station
17 Apparatus 3 – Reciprocating cylinder Advantageseasy to change the pHpH-profileshydrodynamics can be directly influenced by varying the dip rateDisadvantagessmall volume (max. 250 ml)little experiencelimited data
20 Cell typesTablets 12 mm Tablets 22,6 mm Powders / Granules Implants Suppositories / Soft gelatine capsules
21 Apparatus 4 – Flow-Through Cell Advantageseasy to change media pHpH-profile possiblesink conditionsdifferent modes a) open system b) closed systemDisadvantagesDeaeration necessaryhigh volumes of medialabor intensive
23 Apparatus 5 – Paddle over disk Useful fortransdermal patchesStandard volume900 ml
24 Apparatus 5 – Paddle over disk Advantagesstandard equipment (paddle) can be used, only add a stainless steel disk assemblyDisadvantagesdisk assembly restricts patch size
25 Apparatus 6 – Rotating cylinder USP apparatus 7 – Reciprocating holder most probably will be removed from the USP !!!
26 Summary Immediate release dosage forms: apparatus 1 or 2 (preferably 2)Controlled release dosage forms: apparatus 1 or 2 using different media for QC apparatus 3 or 4 for R&D purposesBeside the selection of an adequate dissolution apparatus, adequate test conditions are crucial for all purposes !
28 Calibration Why ? How ? When ? to confirm suitability of the equipment and proper operation of the apparatusHow ?mechanical calibration (verification of physical parameters)chemical calibration („Apparatus Suitability Test“ – USP)When ?before using new test equipmentafter relocation or major maintenanceat regular intervals („every 6 months“)
29 Factors that may affect reliability of the test Proper alignment/geometry of dissolution apparatusdimensions of vessels, paddles, baskets, cylindersheight, centering and wobbleProper conditions during dissolution testtemperatureagitation speeddegassingsampling (sampling zone, timing, filtration, dilution)vibrationProper validation of analytical methodspecified in USP Chapter <1225>
30 Mechanical calibration Verification of physical parameters specified in the pharmacopoeia: USP apparatus 1 and 2
31 Mechanical calibration - Parameters Height – Vertical Position of the Paddle or Basketthe vertical position of paddle or basket affects the hydrodynamics condition in the vesseleach paddle or basket should be individually adjusted to the compendial distancein the pharmacopoeia, a distance of cm is specifieddifferent kinds of height gauges can be used to align or check* this parameter*
32 Mechanical calibration - Parameters Rotational Speed – Stirring Rateinput variable that affects the hydrodynamicschanges in the rotational speed result in a changing liquid-solid interface between the solvent and the dosage formthe rotational speed can be checked by using a digital tachometer*the compendia specify a rotational speed tolerance of + 4 %*
33 Mechanical calibration - Parameters Shaft Wobble – Eccentricity of Stirring Deviceassumed to alter the pattern of fluid movement in both paddle and basket apparatus and therefore may influence the dissolution ratecan be measured with a micrometer*measured is the sum of distance between both sides (180°) of the axis of rotation*
34 Mechanical calibration - Parameters Centering (Vessel / Shaft)the axis of the rotating shaft must coincide at all points with the axis of the vessel to within + 1 mm“the shaft has to positioned so that is axis is not more than 2^mm at any point from the vertical axis of the vessel and rotates smoothly without significant wobble”*
35 Mechanical calibration Measurement toolsall mechanical tools used for calibration should be certified to assure their reliabilitythe results of mechanical calibration have to be documented
36 Apparatus suitability test (USP) if all parts ( apparatus, geometry, test conditions, analytical method) are within compliance – why perform an apparatus suitability test?the apparatus suitability is to check for parameters that can not be conveniently measured (vibration, vessel cleanliness, medium degassing ...) and also to provide an overall check of the system
37 Apparatus suitability test (USP) first established in 1978routine test in most pharmaceutical laboratoriescalibration at regular intervals (every 6 months)standard calibrator substances according USP chapter <711>only the method(s) to be used have to be calibrated !if six units are tested – all have to pass
38 Apparatus suitability test (USP) Standard calibrators according to USP chapter <711>Apparatus I, II and V:disintegrating typeUSP Prednisone Tabletsnondisintegrating typeUSP Salicylic acid TabletsApparatus III:USP Chlorpheniramine Maleate Extended-Release Tablets
40 Apparatus suitability test (USP) USP Prednisone Tablets RS – current lot P0E203(10 mg nominal prednisone content per tablet)disintegrating typepaddle and basket, 50 rpm500 ml deaerated water, 37°Cquantity of prednisone released after 30 minutes is determinedspecified ranges Lot P0E203: Apparatus 1: % Apparatus 2: %
41 Apparatus suitability test (USP) USP Salicylic acid Tablets RS – current lot Q0D200(300 mg nominal salicylic acid content per tablet)nondisintegrating typepaddle and basket, 100 rpm900 ml deaerated phosphate buffer, 37°Cquantity of salicylic acid, released after 30 minutes is determinedspecified ranges Lot Q0D200: Apparatus 1: % Apparatus 2: %
42 Apparatus suitability test (USP) Controversies regarding the current testthe variability in the intrinsic performance of the USP calibrator tablets is so great that it exceeds the variability in intrinsic performance of modern test dissolution assembliesthis variability becomes obvious in both vessel-to-vessel variability and inter-laboratory variability of results for a given lot of calibrators
43 Troubleshooting Calibrator Tablets: always check the incoming tablets !right lot of calibrators ?are the tablets broken, fused or severely chipped ?particularly salicylic acid tablets are often subject to sublimation ( dust on the tablets and the inner surface of the container)use correct storage conditions !take the tablets out of the original container immediately before test !
44 Troubleshooting Standard / Standard solution: USP Standard used ? drying procedure conducted ?standard solution prepared on day of test ?standard solution filtered in the same manner as sample ?amount of alcohol used in the standard < 5% ?
45 Troubleshooting Vibration vibration produces unwanted variation in dissolution data and mostly results in an increased dissolution rateinternal vibration may be caused e.g. from frayed drive beltsexternal vibration may be caused by e.g. magnetic stirrers, centrifuges, vacuum pumps, old fridges, nearby construction, ...inability to properly measure vibration levels at various points within an apparatus is the main reason why calibrator tablets were originally developedVibration is a variable that can seriously distort the data from any dissolution system. Therefore the system must be free of vibration that significantly interferes with the test.Vibration may be caused internal for example from frayed drive belts or the circulation of the water bath but rather by external sources like magnetic stirrers, centrifuges, nearby construction and so on.The inability to properly measure vibration levels at various points within an apparatus was the main reason why calibrator tablets were originally developed.
46 Troubleshooting Vibration effects – case example: Effect of vibration levels of the dissolution apparatus on the dissolution rate of enteric coated granules of Cefalexin. The vertical dotted line indicates 0.05 m/s2.Kaniwa N. et al. (1998) Int J Pharm, 175,„Low vibration“: < 0.05 m/s2„High vibration“: > 0.05 m/s2A possible effect of high vibration levels on the dissolution rate was also shown in a collaborative study of Kaniwa et al. (1998).In order to develop a dissolution standard for evaluating vibration levels for dissolution apparatus, a series of tests, examining enteric coated granules of cefalexin was performed at seven independent laboratories.The vibration of the dissolution apparatus during the test was measured with a vibration meter and the resulting values were plotted against the corresponding release rates of cefalexin. The enteric coated granules worked very well in detection of high vibration levels (especially in the basket apparatus). There seems to be a critical value between so called low and high vibration apparatus at about 0.05 m/s2. Dissolution apparatus that belong to the high vibration category according this classification do not seem to provide accurate and reproducible results!Based on the results of this study, in the event of dissolution results that show unexpectedly high release rates, troubleshooting concerning vibration is crucially important.
47 Troubleshooting Vibration: dissolution equipment placed planar ? drive chain or belt free of tension and/or dirt ?torn parts replaced ?correctly functioning gear plates ?individual spindles are not surging ?bench/table stable ?no sources of vibration nearby ?
48 Troubleshooting Dissolution medium: correctly degassed ? correct amount used (900/500 ml) ?correct amount dosed (weight/volume) ?dosing procedure gentle (resaturation/spillage) ?buffer correct (pH units, buffer salts, molarity) ?correct temperature during test (32°C / 37°C + 0.5°C)?evaporation during test negligible ?
49 Troubleshooting Importance of degassing: insufficient degassing may result in decreased dissolution rates of several drugse.g. prednisone tablets but also a range of poorly soluble drugs are very sensitive to the amount of dissolved gases in the dissolution mediumthe degassing procedure should therefore be efficient and reproducible for every testI really want to point out the importance of degassing, because ….
50 Troubleshooting Deaeration method USP heat the dissolution medium to about 41°Cvacuum filter through a 0.45-µm-porosity membrane into a flask, stirring with a magnetic stirrercontinue to draw a vacuum and stir for an additional 5 mingently transfer the medium directly into the vesselrotating the apparatus 2 shafts to speed equilibration to 37°C is discouraged!!!use medium promptly after equilibrationAn adequate dearation method is decribed in the USP:First, a suitable volume of medium is heated to 41°C. With the aid of vacuum it is filtered through a 0.45 µm porosity membrane into a filtering flask equipped with a stirring device. The flask is sealed and while stirring for an additional 5 minutes, the vacuum is been continued. Then the medium is gently transferred directly into the vessel and promptly used after equilibration to a temperature of 37°C.
51 Troubleshooting Alternative deaeration methods the USP states that „other validated deaeration techniques for removal of dissolved gases may be used“other techniques include:heatingsonicationvacuumhelium sparging (expensive)
52 Troubleshooting Sampling take each sample at the correct time point sampling time points (+ 2%)use a single glass syringe for each vesselsample from the right location within the vessel between media surface and top of the paddle blade n.l.t. 10 mm from vessel wall
53 Troubleshooting Sampling always use a suitable filter check filter adsorptioncheck the clearity of the filtered samplefilter the sample immediately after samplingfor automated sampling also check the tubings
54 Troubleshooting Physical conditions of the apparatus vessels scrupulously clean ?vessel surface smooth and curvature appropriate ?Apparatus 1the conditions of the baskets, particularly of their clips is criticalcheck all baskets for corrosion and blocked meshes before using themalign the air holes to prevent air cushions emerging during the test
55 (Semi) Automation Advantages high throughput of samples minimizes analyst-to-analyst variability in sampling and filtrationreduces the average costs per analysisvery promising for QC purposes
56 Media preparation automated mixing of water and concentrate preheating of mediumdeaeration (vacuum and stirring)media can be dispensed directly into the vesselIn this slide you can see a media preparation system that offers you a time- and effort- saving way of mixing, preheating, deaerating and dipensing of your medium.The test medium can be mixed from concentrate (buffer or acid) and water, preheated to a defined temperature, deaerated under vacuum over a particular time and then be directly dipensed into the vessel.Altogether this procedure should result in a reproducible degassing process.
57 Offline systemHere you can see an Offline system for apparatus 1 and 2. This system consists of a dissolution bath, coupled with a sampling unit. Samples can be taken at predetermined time points. If necessary they can be diluted immediately and after that collected in test tubes for UV-analysis or in HPLC vials respectively.
58 Online systemIf you have a high throughput of samples that do not require dilution before UV analysis, a so-called Online system will make sense. This system consists of a dissolution bath that is directly coupled with an UV-spectrophotometer. Using this setup, it is possible to record multiple-point (real time) dissolution curves using a flow through-cuvette-system (closed system).
59 On- / Offline systemA combination of On- and Offline System combines two major advantages of single On- and Offline System by offering the possibility of an online-UV-measurement of automatically diluted samples.The system also can be run in single offline or online mode like described before.
60 HPLC - online systemA further possibility for online-detection is represented by an HPLC – online system that combines the dissolution bath with a complete HPLC system. This system would be helpful in case of a high throughput in samples that cannot be measured by simple UV-analysis. For example this will be the case as you have excipients that would interfere with perturb UV-analysis or as you use biorelevant media like e.g. FaSSIF or FeSSIF as test media.
61 Automationalways validate automated methods, including analytical and sampling methodsvalidation should be performed using manual analysis, withdrawing samples at the same times and comparing to the automated results:not highly variable dissolution results: two concurrent runshighly variable dissolution results: simultaneous samplingpay attention to automated dilution and filtering processesWhat do you have to consider when thinking about (semi-) automation?Automated methods always have to be validated. The validation should include analytical and sampling methods. Validation should be performed using manual analysis, withdrawing samples at the same times and comparing to automated results.If the dissolution results are not highly variable, it is possible to compare two concurrent runs. If they are highly variable, simultaneous sampling has to take place.Special attention should be paid to automated dilution and filtering processes!
62 Suggested readingFIP Guidelines for dissolution testing of solid oral products. Dissolution Technologies 4:5-14 (1997).SM Diebold, JB Dressman. Dissolved oxygen as a measure for de- and re-aeration of aqueous media for dissolution testing. Dissolution Technologies 5: (1998).S Qureshi. Calibration – the USP dissolution apparatus suitability test. Drug Inf. J. 30, (1996).N Kaniwa et al. Collaborative study on the development of a standard for evaluation of vibration levels for dissolution apparatus. Int. J. Pharm. 175: (1998).VA Gray, CK, Brown, JB Dressman, LJ Leeson. A new general information chapter on dissolution. Pharmacopoeial Forum 27 (6) [Nov.-Dec. 2001]W Brown. General information <1092> The dissolution procedure: development and validation Pharmacopoeial Forum 30 (1) [Jan.-Feb. 2004]Of general interest:Dissolution Technologies:With this slide, I want to end my talk.You can see some references for more information regarding calibration of dissolution systems.It is also worth to have a view at the homepage of „Dissolution technologies“. This journal is published quarterly and contains a lot of useful informations for all areas of dissolution testing.
63 Johann Wolfgang Goethe University Dr. Sandra KleinJohann Wolfgang Goethe UniversityInstitute of Pharmaceutical Technology9 Max von Laue StreetFrankfurt, 60438, GermanyWith this slide, I want to end my talk.You can see some references for more information regarding calibration of dissolution systems.It is also worth to have a view at the homepage of „Dissolution technologies“. This journal is published quarterly and contains a lot of useful informations for all areas of dissolution testing.