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Figure 1 Reactivity of the patients' antibodies with rat brain and HEK cell-based assays Rat hippocampal dentate gyrus neuropils were stained with patient.

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Presentation on theme: "Figure 1 Reactivity of the patients' antibodies with rat brain and HEK cell-based assays Rat hippocampal dentate gyrus neuropils were stained with patient."— Presentation transcript:

1 Figure 1 Reactivity of the patients' antibodies with rat brain and HEK cell-based assays Rat hippocampal dentate gyrus neuropils were stained with patient CSF; the reactivity was revealed using anti-human immunoglobulin G (IgG) (in green) or immunoglobulin A (IgA) (in red) (A–F, scale bar = 50 μm). Reactivity of the patients' antibodies with rat brain and HEK cell-based assays Rat hippocampal dentate gyrus neuropils were stained with patient CSF; the reactivity was revealed using anti-human immunoglobulin G (IgG) (in green) or immunoglobulin A (IgA) (in red) (A–F, scale bar = 50 μm). The CSF of the first patient (A–C) contained both IgG–NMDA receptor (NMDAR)–antibodies (Abs) (A) and IgA-NMDAR-Abs (B), both showing the same pattern of neuropil staining (C). The CSF of the second patient (D–F) only contained IgG-NMDAR-Abs (D). No IgA-NMDAR-Abs was detected (E, F). Human epithelial kidney (HEK) cells were transfected to express green fluorescent protein (GFP)–tagged GluN1 (green; G, J) and were then incubated with CSF from patients; the reactivity was revealed using anti-human IgG (red; H) or anti-human IgA (red; K) (G–L, scale bars = 10 μm). (I, L) The merged image of GFP-GluN1 and IgG or IgA reactivity, respectively. The nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Virginie Desestret et al. Neurol Neuroimmunol Neuroinflamm 2015;2:e166 © 2015 American Academy of Neurology

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