1. From: Skeletal Muscle Ryanodine Receptor Mutations Associated with Malignant Hyperthermia Showed Enhanced Intensity and Sensitivity to Triggering Drugs when Expressed in Human Embryonic Kidney Cells
Anesthes. 2013;119(1): doi: /ALN.0b013e31828cebfe
Figure Legend:
Immunofluorescence for transfected human embryonic kidney (HEK)-293 cells expressing the large T-antigen of simian virus 40 (HEK-293T) cells. HEK-293T cells on a glass slide were transfected with wild-type (WT) or R163C mutant skeletal muscle ryanodine receptor (RYR1) complementary DNAs. The expressed RYR1 was detected by 34C and fluorescein isothiocyanate-conjugated secondary antibody (green), and protein disulfide isomerase proteins were detected by antiprotein disulfide isomerase and tetramethylrhodamine isothiocyanate-conjugated secondary antibody (red) as an endoplasmic reticulum (ER) marker. Nuclei were stained using 4′,6-diamidino-2-phenylindole (blue). Fluorescence was observed using a BX51 fluorescence microscope (Olympus, Tokyo, Japan) using the 100× objective and filter cubes U-MWU2, U-MWIBA2, U-MWIG2, or U-61000V2. Transient transfection at low density of cells induces RYR1 expression only in a few cells, showing the difference between cells with and without RYR1 proteins. The scale bar represents 50 µm.
Date of download: 11/9/2017
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