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Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK
Tune file - nanospray
Micro AS autosampler settings Note: Micro AS must be put back to Menu/Serial (use the touch screen) Note: in sample table the vial position should be 1a1
Micro AS autosampler settings Wash solution: water
Micro AS autosampler settings
Surveyor LC settings
LTQ Orbitrap settings
Exclusion duration set to about ½ peak width (here 15 sec)
LTQ Orbitrap settings
Scan event 2 – MS/MS of the 1 st most intense ion
LTQ Orbitrap settings Scan event 3 – MS/MS of the 2 nd most intense ion
LTQ Orbitrap settings Scan event 4 – MS/MS of the 3 rd most intense ion
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1 The world leader in serving science LTQ Orbitrap Series: Calibration, Tuning, Operations I Orbi
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