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Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK.

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Presentation on theme: "Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK."— Presentation transcript:

1 Details of Tune and Method Setup for LTQ Orbitrap Application: nanospray, peptides Gary Woffendin, Michaela Scigelova Thermo Electron, UK

2 Tune file - nanospray

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10 Micro AS autosampler settings Note: Micro AS must be put back to Menu/Serial (use the touch screen) Note: in sample table the vial position should be 1a1

11 Micro AS autosampler settings Wash solution: water

12 Micro AS autosampler settings

13 Surveyor LC settings

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15 LTQ Orbitrap settings

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18 Exclusion duration set to about ½ peak width (here 15 sec)

19 LTQ Orbitrap settings

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23 Scan event 2 – MS/MS of the 1 st most intense ion

24 LTQ Orbitrap settings Scan event 3 – MS/MS of the 2 nd most intense ion

25 LTQ Orbitrap settings Scan event 4 – MS/MS of the 3 rd most intense ion

26 Divert Valve

27 Method Summary

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