1. Details of Tune and Method Setup for LTQ Orbitrap
Application: nanospray, peptides
Gary Woffendin, Michaela Scigelova
Thermo Electron, UK

2. Tune file - nanospray

3. Tune file - nanospray

4. Tune file - nanospray

5. Tune file - nanospray

6. Tune file - nanospray

7. Tune file - nanospray

8. Tune file - nanospray

9. Tune file - nanospray

10. Micro AS autosampler settings
Note: Micro AS must be put back to ‘Menu/Serial’ (use the touch screen)
Note: in sample table the vial position should be ‘1a1’

11. Micro AS autosampler settings
Wash solution: water

12. Micro AS autosampler settings

13. Surveyor LC settings

14. Surveyor LC settings

15. LTQ Orbitrap settings

16. LTQ Orbitrap settings

17. LTQ Orbitrap settings

18. LTQ Orbitrap settings
Exclusion duration set to about ½ peak width (here 15 sec)

19. LTQ Orbitrap settings

20. LTQ Orbitrap settings

21. LTQ Orbitrap settings

22. LTQ Orbitrap settings

23. LTQ Orbitrap settings
Scan event 2 – MS/MS of the 1st most intense ion

24. LTQ Orbitrap settings
Scan event 3 – MS/MS of the 2nd most intense ion

25. LTQ Orbitrap settings
Scan event 4 – MS/MS of the 3rd most intense ion

26. Divert Valve

27. Method Summary

28. Method Summary