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1 The world leader in serving science Proprietary & Confidential WiSIM DIA Method Set-up Orbitrap Fusion MS MS Instrument Control Software v.1.1 SP1.

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Presentation on theme: "1 The world leader in serving science Proprietary & Confidential WiSIM DIA Method Set-up Orbitrap Fusion MS MS Instrument Control Software v.1.1 SP1."— Presentation transcript:

1 1 The world leader in serving science Proprietary & Confidential WiSIM DIA Method Set-up Orbitrap Fusion MS MS Instrument Control Software v.1.1 SP1

2 2 WiSIM DIA, how does it work? Unique to the Orbitrap Fusion MS: Three HR/AM SIM are used to cover precursors ions from 400 to 1000 m/z MS1 ultra high resolution quantification In parallel with each HR/AM SIM scan, 17 sequential Ion Trap CID MS/MS with 12 amu isolation windows are acquired to cover the associated 200 amu HR/AM SIM mass range MS2 confirmation SIM amuSIM amuSIM amu tSIM Orbitrap tMS2 Ion Trap

3 3 WiSIM DIA, how does it work? SIM 400 – 600 m/z MS2 No. 1 … MS2 No.17 1 st MS1 SIM 600 – 800 m/z Ion Trap OT 240k 3.6 sec SIM 800 – 1000 m/z 2 nd MS1 MS2 No. 1 … MS2 No.17 Ion Trap 3 rd MS1 MS2 No. 1 … MS2 No.17 Ion Trap OT 240k OT 240k 17 Windows of 12 amu each 17 Windows of 12 amu each 17 Windows of 12 amu each

4 4 WiSIM DIA Method Set-up at a glance: SIM , , m/z 200 amu ion trap isolation width AGC: 3e4 50 ms Max injection Profile tMS2 12 amu quadrupole isolation m/z mass range Ion Trap detection AGC: 5e4 35 ms Max Injection CID 30 Rapid Scan rate X3 6 experiments:

5 5 WiSIM DIA Method Set-up: 6 experiments: -Three HR/AM tSIM -Three Ion trap tMS2 CID

6 6 Experiment 1: SIM amu SIM amuSIM amu

7 7 Experiment 2: SIM amuSIM amuSIM amu

8 8 Experiment 2: SIM amuSIM amuSIM amu

9 9 Experiment 3: SIM amu SIM amu SIM amu

10 10 Experiment 4: SIM amuSIM amuSIM amu

11 11 Experiment 4: SIM amuSIM amuSIM amu

12 12 Experiment 5: SIM amuSIM amu SIM amu

13 13 Experiment 6: SIM amuSIM amuSIM amu

14 14 Experiment 6: SIM amuSIM amuSIM amu

15 15 WiSIM-DIA advantages Low detection limits, high selectivity is achieved using 240,000 SIM scan. High sensitivity Ion Trap CID MS2 is used for confirmation of the low abundant components. Unsurpassed speed with 2 detectors working in parallel allows for 3.6 s cycle. LOD and LOQ down to 10 attomole. CVs are better than 15 % for 85 % of compounds, better than 20% for 95% of compounds. Linear dynamic range up to 4 orders of magnitude.

16 16 For More details, please see Application Note 600 And:


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