Presentation is loading. Please wait.

Presentation is loading. Please wait.

Age-Related Differences in Sensitivity of Peripheral Blood Monocytes to Lipopolysaccharide and Staphylococcus Aureus Toxin B in Atopic Dermatitis  Marie.

Published by Modified over 5 years ago

Similar presentations

Presentation on theme: "Age-Related Differences in Sensitivity of Peripheral Blood Monocytes to Lipopolysaccharide and Staphylococcus Aureus Toxin B in Atopic Dermatitis  Marie."— Presentation transcript:

1 Age-Related Differences in Sensitivity of Peripheral Blood Monocytes to Lipopolysaccharide and Staphylococcus Aureus Toxin B in Atopic Dermatitis  Marie Mandron, Marie-Françoise Ariès, Franck Boralevi, Hélène Martin, Marie Charveron, Alain Taieb, Christian Davrinche  Journal of Investigative Dermatology  Volume 128, Issue 4, Pages (April 2008) DOI: /sj.jid Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Activation of CD14+ monocytes by SEB. (a) Monocytes isolated from non-atopic subjects were treated with increasing concentration of SEB or LPS (0–1,000ngml−1). After 24hours of incubation, HLA-DR expression was analyzed by flow cytometry, and histograms of MFI are shown. (b) Graphic representation of dose–response to stimulations with SEB or LPS. Averages from all the patients tested with standard deviations are shown. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Phenotype of SEB-activated monocytes. Monocytes were cultured in the presence of 10ngml−1 of SEB or LPS or left untreated (NT) for 24hours. HLA-DR, CD54, CD80, and CD86 surface expression was analyzed by flow cytometry as described in the Materials and Methods section. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Modulation of TLR2 and TLR4 expression on activated monocytes. (a) Monocytes were either untreated (NT) or incubated for 24hours with 10ngml−1 of SEB or LPS. Surface expression of TLR2 and TLR4 was analyzed by flow cytometry, and histograms of MFI are shown. (b) Monocytes were preincubated with neutralizing antibodies to TLR2 or TLR4 or isotypic control. Cells were treated for 24hours with 10ngml−1 of SEB or LPS, and TNF-α secretion was analyzed by ELISA on culture supernatants. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Activation of monocytes from atopic (AD) patients. Monocytes from 30AD patients (15 adults and 15 children) or 15 non-atopic controls as indicated were cultured for 24hours in the presence of 10ngml−1 of SEB or LPS. Surface expression of HLA-DR was analyzed by flow cytometry, and histograms of MFI values are shown after deduction of MFI values corresponding to isotypic controls. Mean values of MFI for each experiment are artificially connected and compared according to paired Student's t-test (*P<0.005, **P<0.001, NS, not significant). Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Production of cytokines by atopic monocytes. Monocytes isolated from AD patients and non-atopic controls were treated with 10ngml−1 of SEB or LPS. Supernatants collected after 24hours of incubation were analyzed for cytokine production using cytometry bead array assay. Histograms show concentration (pgml−1) for IL-6, IL-10, and TNF-α cytokines secreted by monocytes from 10 different adult individuals either non-atopic (NAD) or atopic (AD). Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 Modulation of TLR2 and TLR4 expression on atopic monocytes. Cultures of monocytes from 30AD patients or 15 non-atopic controls were either untreated (NT) or cultured in the presence of 10ngml−1 of SEB or LPS for 24hours. Surface expression of TLR2 and TLR4 was analyzed by flow cytometry using specific monoclonal antibodies. Histograms of MFI values are shown after deduction of MFI values corresponding to isotypic controls. Mean values of MFI for each experiment are artificially connected and compared according to paired Student's t-test (**P<0.001, NS, not significant). Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

8 Figure 7 Production of IL-12 by atopic monocytes. Monocytes isolated from 30AD patients (15 adults and 15 children) and 15 non-atopic controls were either untreated (NT) or treated with 10ngml−1 of SEB or LPS for 24hours. Secretion of IL-12p70 was subsequently analyzed by ELISA on culture supernatants and compared according to paired Student's t-test. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Age-Related Differences in Sensitivity of Peripheral Blood Monocytes to Lipopolysaccharide and Staphylococcus Aureus Toxin B in Atopic Dermatitis  Marie."

Similar presentations

Volume 94, Issue 2, Pages (March 2014)

Volume 94, Issue 2, Pages (March 2014)
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 
In situ Delivery of Tumor Antigen– and Adjuvant-Loaded Liposomes Boosts Antigen- Specific T-Cell Responses by Human Dermal Dendritic Cells  Martine A.

In situ Delivery of Tumor Antigen– and Adjuvant-Loaded Liposomes Boosts Antigen- Specific T-Cell Responses by Human Dermal Dendritic Cells  Martine A.
Nickel Sulfate Promotes IL-17A Producing CD4+ T Cells by an IL-23-Dependent Mechanism Regulated by TLR4 and Jak-STAT Pathways  Rami Bechara, Diane Antonios,

Nickel Sulfate Promotes IL-17A Producing CD4+ T Cells by an IL-23-Dependent Mechanism Regulated by TLR4 and Jak-STAT Pathways  Rami Bechara, Diane Antonios,
Nickel Sulfate Promotes IL-17A Producing CD4+ T Cells by an IL-23-Dependent Mechanism Regulated by TLR4 and Jak-STAT Pathways  Rami Bechara, Diane Antonios,

Nickel Sulfate Promotes IL-17A Producing CD4+ T Cells by an IL-23-Dependent Mechanism Regulated by TLR4 and Jak-STAT Pathways  Rami Bechara, Diane Antonios,
The CXC-chemokine platelet factor 4 promotes monocyte survival and induces monocyte differentiation into macrophages by Barbara Scheuerer, Martin Ernst,

The CXC-chemokine platelet factor 4 promotes monocyte survival and induces monocyte differentiation into macrophages by Barbara Scheuerer, Martin Ernst,
CD271 on Melanoma Cell Is an IFN-γ-Inducible Immunosuppressive Factor that Mediates Downregulation of Melanoma Antigens  Junpei Furuta, Takashi Inozume,

CD271 on Melanoma Cell Is an IFN-γ-Inducible Immunosuppressive Factor that Mediates Downregulation of Melanoma Antigens  Junpei Furuta, Takashi Inozume,
Interleukin-10 Downregulates Anti-Microbial Peptide Expression in Atopic Dermatitis  Michael D. Howell, Natalija Novak, Thomas Bieber, Saveria Pastore,

Interleukin-10 Downregulates Anti-Microbial Peptide Expression in Atopic Dermatitis  Michael D. Howell, Natalija Novak, Thomas Bieber, Saveria Pastore,
Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.
Impaired Responses of Peripheral Blood Mononuclear Cells to Staphylococcal Superantigen in Patients with Severe Atopic Dermatitis: A Role of T Cell Apoptosis 

Impaired Responses of Peripheral Blood Mononuclear Cells to Staphylococcal Superantigen in Patients with Severe Atopic Dermatitis: A Role of T Cell Apoptosis 
IL-36γ Is Involved in Psoriasis and Allergic Contact Dermatitis

IL-36γ Is Involved in Psoriasis and Allergic Contact Dermatitis
Wanhong Ding, John A. Wagner, Richard D. Granstein 

Wanhong Ding, John A. Wagner, Richard D. Granstein 
Staphylococcus aureus Stimulates Neutrophil Targeting Chemokine Expression in Keratinocytes through an Autocrine IL-1α Signaling Loop  Florina Olaru,

Staphylococcus aureus Stimulates Neutrophil Targeting Chemokine Expression in Keratinocytes through an Autocrine IL-1α Signaling Loop  Florina Olaru,
IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis  Rahul Purwar, Thomas Werfel, Miriam.

IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis  Rahul Purwar, Thomas Werfel, Miriam.
Cytokine Effects Induced by the Human Autoallergen α-NAC

Cytokine Effects Induced by the Human Autoallergen α-NAC
Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes  Marcel B.M. Teunissen, Jan.

Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes  Marcel B.M. Teunissen, Jan.
Human Keratinocytes Express Functional CD14 and Toll-Like Receptor 4

Human Keratinocytes Express Functional CD14 and Toll-Like Receptor 4
Propionibacterium acnes Induces an IL-17 Response in Acne Vulgaris that Is Regulated by Vitamin A and Vitamin D  George W. Agak, Min Qin, Jennifer Nobe,

Propionibacterium acnes Induces an IL-17 Response in Acne Vulgaris that Is Regulated by Vitamin A and Vitamin D  George W. Agak, Min Qin, Jennifer Nobe,
Volume 141, Issue 3, Pages (September 2011)

Volume 141, Issue 3, Pages (September 2011)
Streptococcus Induces Circulating CLA+ Memory T-Cell-Dependent Epidermal Cell Activation in Psoriasis  Marta Ferran, Ana B. Galván, Catalina Rincón, Ester.

Streptococcus Induces Circulating CLA+ Memory T-Cell-Dependent Epidermal Cell Activation in Psoriasis  Marta Ferran, Ana B. Galván, Catalina Rincón, Ester.

Similar presentations

Ads by Google