1. Volume 94, Issue 2, Pages 123-130 (March 2014)
Pleural fluid mononuclear cells (PFMCs) from tuberculous pleurisy can migrate in vitro in response to CXCL10 
Changyou Wu, Jiangjun Ma, Yanquan Xu, Xianlan Zhang, Suihua Lao, Binyan Yang 
Tuberculosis 
Volume 94, Issue 2, Pages (March 2014)
DOI: /j.tube
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2. Figure 1
The expression of chemokine receptors on T cells from PFMCs and PBMCs. The expression of CXCR3, CCR4, CCR6 and CCR7 on CD3+CD4+ and CD3+CD8+ T cells from PFMCs (A, n = 3) and PBMCs (B, n = 3) were analyzed by flow cytometry. Dark histogram: control; white histogram: cell surface staining. (C). Percentage of chemokine receptors on CD3+, CD4+ and CD8+ T cells from PFMCs and PBMCs. Data are expressed as mean ± SD, whereas error bars represent of three independent experiments with the similar results. *P < 0.05, * *P < 0.01, ns: not significant.
Tuberculosis  , DOI: ( /j.tube )
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3. Figure 2
The levels of CXCL10 in PFMCs fluids (TBPF) and the production of CXCL10 in PFMCs and PBMCs after stimulation with BCG. (A). High levels of CXCL10 protein in tubercular pleural fluid. Scatter-plot showing CXCL10 levels in patients with tuberculous pleurisy (n = 63), cancerous pleurisy (n = 12) and normal serum (n = 15). Each symbol represents the value for an individual person and horizontal bars represent the medians. (B). BCG induced the production of CXCL10 by PFMCs and PBMCs. Levels of CXCL10 protein in BCG-stimulated PFMCs (n = 13) and PBMCs (n = 13) were measured by ELISA. *P < 0.05, * *P < 0.01, ns: not significant.
Tuberculosis  , DOI: ( /j.tube )
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4. Figure 3
The levels of CXCL10 and IFN-γ in the tubercular pleural fluid and IFN-γ induced the production of CXCL10 by PFMCs and PBMCs. A. The correlation between CXCL10 and IFN-γ in the TBPF (n = 36). Statistical significance and correlations were determined with the spearman test (R2 = 0.7013). P <  B and C. IFN-γ induced the production of CXCL10 by PFMCs and PBMCs. Cell culture supernatants were collected at different time points after stimulation and analyzed for CXCL10 secretion by ELISA. Dose- and time-dependent expression of CXCL10 by PFMCs (B, n = 5) and PBMCs (C, n = 5) stimulated with IFN-γ. * *P < 0.01.
Tuberculosis  , DOI: ( /j.tube )
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5. Figure 4
The expression of CXCL10 on CD3+, CD14+, CD16b+ cells from PFMCs and PBMCs and the production of CXCL10 in monocytes and neutrophils after stimulation with IFN-γ. A. The cells were cultured in medium alone or stimulated with IFN-γ (10 ng/ml) for 12 h. After stimulation, cells were harvested and FACS staining was conducted (A, n = 5) Dark histogram: control, white histogram: intracellular cytokine staining. B and C. IFN-γ induced the production of CXCL10 by monocytes (B, n = 3) and neutrophils (C, n = 5). Cell culture supernatants were collected at different time points after stimulation and analyzed for CXCL10 secretion by ELISA. Dose- and time-dependent expression of CXCL10 by monocytes and neutrophils stimulated with IFN-γ. * *P < 0.01.
Tuberculosis  , DOI: ( /j.tube )
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6. Figure 5
Anti-IFN-γ antibody inhibited the production of CXCL10 by PFMCs and PBMCs. Levels of CXCL10 protein in BCG-stimulated PFMCs (A, n = 3) and PBMCs (B, n = 3) with or without neutralizing anti-IFN-γ antibody or an isotype-matched control antibody for 12 h. CXCL10 in the culture supernatant was measured by ELISA. *P < 0.05, ns: not significant.
Tuberculosis  , DOI: ( /j.tube )
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7. Figure 6
Production of IFN-α and IFN-α induced the production of CXCL10. A. BCG induced the production of IFN-α by PFMCs and PBMCs. PFMCs and PBMCs were incubated with BCG for 8 h and the levels of for IFN-α were measured by StepOnePlus Real Time PCR system and used SYBR Green qPCR Mastermix. GAPDH served as an internal control. The similar results were in an additional three experiments. * *P < 0.01. B. BCG induced the expression of IFN-α by neutrophils, monocytes and non-monocytes. Neutrophils, monocytes and non-monocytes were treated with BCG and the levels of IFN-α mRNA were measured by StepOnePlus Real Time PCR system. The similar results were in an additional three experiments. * *P < 0.01. C. The levels of IFN-α in the supernatants of cultures of PFMCs that had been stimulated with BCG were measured (n = 5). BCG was added to the culture medium of PFMCs at different concentrations (1.25–20 μg/ml) and cultured for 12 h, 24 h, and 48 h. IFN-α induced the production of CXCL10 by PFMCs (D, n = 5), PBMCs (E, n = 5), monocytes (F, n = 5) and neutrophils (G, n = 5). Dose- and time-dependent expression of CXCL10 by PFMCs, PBMCs, monocytes and neutrophils stimulated with IFN-α. Cell culture supernatants were collected at different time points after stimulation and analyzed for CXCL10 secretion by ELISA. Each symbol represents the value for an individual person and horizontal bars represent the medians. *P < 0.05, * *P < 0.01. Anti-IFN-α antibody inhibited the production of CXCL10 by PFMCs (H, n = 3) and PBMCs (I, n = 6). *P < 0.05, * *P < 0.01, ns: not significant.
Tuberculosis  , DOI: ( /j.tube )
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8. Figure 7
CXCL10 induced the migration of PFMCs. PFMCs from TP patients were incubated in presence or absence of CXCL10, anti-CXCL10 and TBPF for 2 h, 4 h and 6 h in a trans-well migration assay. The number of cells migration to the lower chamber was determined by counting under microscopy. Data are expressed as mean ± SD, and error bars represent of three independent experiments with the similar results. * *P < 0.01, ns: not significant.
Tuberculosis  , DOI: ( /j.tube )
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