1. Pathogenic potential of naïve Helicobacter-reactive CT6 cells in lymphopenic mice.
Pathogenic potential of naïve Helicobacter-reactive CT6 cells in lymphopenic mice. Six-week-old Rag1−/− mice were given H. apodemus with or without naïve CT6 T cell transfer. H. apodemus was gavaged for a total of three times every other day. Naïve CT6 cells (105) were transferred at the time of last gavage. (A) Flow cytometry analysis for the frequency of Tg cells among the host CD45+ cells [P = CT6 versus CT6 + H. apodemus, one-way analysis of variance (ANOVA) with Tukey’s post hoc test; n = 3, 5, 5, and 5] and development of Treg (Foxp3+) and Teff (CD44hi CD62Llo Foxp3−) cell markers (n = 5) in dMLN at 6 weeks. (B) Colon weight/length ratio at 6 weeks [P = (no treatment versus CT6 + H. apodemus), P = (CT6 versus CT6 + H. apodemus), P = (H. apodemus versus CT6 + H. apodemus), one-way ANOVA with Tukey’s post hoc test; n = 3, 5, 5, and 5]. (C) Representative hematoxylin and eosin (H&E)–stained section of the ascending colon at 6 weeks (original magnification, ×10) and quantification of crypt number [P = (no treatment versus CT6 + H. apodemus), P = (CT6 versus CT6 + H. apodemus), P = (H. apodemus versus CT6 + H. apodemus), one-way ANOVA with Tukey’s post hoc test; n = 3]. The number of crypts observed per 100 μm of ascending colon was averaged from five fields. Decreased crypt number reflects crypt dropout due to inflammation. Bars indicate mean ± SEM. **P < 0.01, ***P < 0.001, ****P <
Jiani N. Chai et al. Sci. Immunol. 2017;2:eaal5068
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