1. Modulation of the BCL-2/BAX ratio by interferon-γ and hypoxia in human peritoneal and adhesion fibroblasts 
Ghassan M. Saed, Ph.D., Zhongliang Jiang, M.D., Ph.D., Nicole M. Fletcher, B.Sc., Michael P. Diamond, M.D. 
Fertility and Sterility 
Volume 90, Issue 5, Pages (November 2008)
DOI: /j.fertnstert
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Multiplex RT/PCR of BCL-2/BAX in human peritoneal and adhesion fibroblasts in response to hypoxia. Multiplex RT/PCR of BCL-2, BAX, and β-actin was performed as described in Materials and Methods using total RNA isolated from normal human peritoneal and adhesion fibroblasts treated with hypoxia (2% O2) for 0, 3, 6, 12, and 24 hours. Polymerase chain reaction products were analyzed on a 2% agarose gel and visualized by ethidium bromide staining. The density of each band was measured by a scanning densimeter. Relative pixel densities corrected to internal control (β-actin) were used to determine the effect of treatments on BCL-2/BAX mRNA ratio. Error bars represent the SEM of triplicates of five subjects (P<.05).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Multiplex RT/PCR of BCL-2/BAX in human peritoneal and adhesion fibroblasts in response to IFN-γ treatment. Multiplex RT/PCR of BCL-2, BAX, and β-actin was performed as described in Materials and Methods using total RNA isolated from normal human peritoneal and adhesion fibroblasts treated with 0, 100, 500, 1000 IU/mL IFN-γ. Polymerase chain reaction products were analyzed on a 2% agarose gel and visualized by ethidium bromide staining. The density of each band was measured by a scanning densimeter. Relative pixel densities corrected to internal control (β-actin) were used to determine the effect of treatments on BCL-2/BAX mRNA ratio. Error bars represent the SEM of triplicates of five subjects (P<.05).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Multiplex RT/PCR of BCL-2/BAX in human peritoneal fibroblasts in response to increasing IFN-γ concentration and hypoxia. Multiplex RT/PCR of BCL-2/BAX and β-actin was performed as described in Materials and Methods using total RNA isolated from normal human peritoneal and adhesion fibroblasts treated with 24 hours' hypoxia and 0, 100, 500, 1000 IU/mL IFN-γ. Polymerase chain reaction products were analyzed on a 2% agarose gel and visualized by ethidium bromide staining. The density of each band was measured by a scanning densimeter. Relative pixel densities corrected to internal control (β-actin) were used to determine the effect of treatments on BCL-2/BAX mRNA ratio. Error bars represent the SEM of triplicates of five subjects (P<.05).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions