Presentation is loading. Please wait.

Presentation is loading. Please wait.

Novel Tissue-Engineered Biodegradable Material for Reconstruction of Vascular Wall  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Satoshi Taketani, MD, Kei Torikai,

Published byGrethe Brekke Modified over 5 years ago

Similar presentations

Presentation on theme: "Novel Tissue-Engineered Biodegradable Material for Reconstruction of Vascular Wall  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Satoshi Taketani, MD, Kei Torikai,"— Presentation transcript:

1 Novel Tissue-Engineered Biodegradable Material for Reconstruction of Vascular Wall 
Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Satoshi Taketani, MD, Kei Torikai, MD, Koichiro Hirakawa, MS, Hikaru Matsuda, MD  The Annals of Thoracic Surgery  Volume 80, Issue 5, Pages (November 2005) DOI: /j.athoracsur Copyright © 2005 The Society of Thoracic Surgeons Terms and Conditions

2 Fig 1 Formation of a biodegradable scaffold reinforced with woven polylactic acid mesh (arrow) cross-linked with collagen-microsponge (A). Scanning electron microscopy image of the tissue-engineered patch shows the uniformly distributed and interconnected pore structure (pore size 50–150 μm) of the collagen-microsponge (B) (magnification 40×). The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2005 The Society of Thoracic Surgeons Terms and Conditions

3 Fig 2 The attachment and proliferation of seeded cells cultured on the polyglycolic acid (PGA) mesh with collagen-microsponge were significantly higher than when the culture was done on PGA mesh with a simple collagen-coat or on PGA mesh alone. * = p < 0.05 versus PGA mesh only; ** = p < 0.05 versus PGA mesh with collagen-coat and PGA mesh only; ♦ = PGA mesh only; ■ = PGA mesh with collagen-coat; ▴ = PGA mesh with collagen-microsponge. (MTT = 3-[4.5 dimethylthiazol 2-yl]-2,5-diphenyltetrazolium bromide.) The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2005 The Society of Thoracic Surgeons Terms and Conditions

4 Fig 3 Macroscopic and histologic findings of the descending aorta model, 2 months after implantation. Macroscopically, there was no aneurysm formation in any animal (A) and (B). The CD31 (C) and α-smooth muscle actin staining (D) showed uniform cellularization of the endothelial and smooth muscle cells (magnification 100×). The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2005 The Society of Thoracic Surgeons Terms and Conditions

5 Fig 4 Macroscopic (A and B) and histologic (C–E) findings of the pulmonary trunk model 1 month and 2 months after implantation. Macroscopically, there was no thrombus formation or neointimal thickness in any sample (A and B). Hematoxylin-eosin staining (C) (magnification 100×) showed in situ cellularization by 1 month (1 month [C]). The CD31 staining (D) (100×) showed a monolayer of endothelial cells by 1 month (1 month [D]). Alpha-smooth muscle actin staining (E) (100×) revealed the parallel alignment of smooth muscle cells at 2 months (2 months [E]). The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2005 The Society of Thoracic Surgeons Terms and Conditions

6 Fig 5 Right ventricular angiography of the large patch model. Arrow indicates tissue engineered patch. There was no stenosis or aneurysmal change from the right ventricular outflow tract to the pulmonary artery. The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2005 The Society of Thoracic Surgeons Terms and Conditions

7 Fig 6 Histologic and scanning electron microscopy findings of large tissue engineered patch (TEP) model 6 months after implantation. Hematoxylin-eosin staining (magnification 100×) showed that the architecture of the implanted TEP was similar to that of the native pulmonary artery wall (A). The scanning electron microscopy observations (500×) showed that the luminal surface of the TEP was uniformly covered by a confluent endothelium (B). Elastica van Gieson staining (100×) revealed the expansion of native tissue into the TEP (C). Factor VIII staining (100×) showed a number of microvessels in the regenerated tissue (D). The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur ) Copyright © 2005 The Society of Thoracic Surgeons Terms and Conditions

Download ppt "Novel Tissue-Engineered Biodegradable Material for Reconstruction of Vascular Wall  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Satoshi Taketani, MD, Kei Torikai,"

Similar presentations

Prevention of Postoperative Pericardial Adhesions With a Novel Regenerative Collagen Sheet  Hiroyuki Tsukihara, MD, Shinichi Takamoto, MD, PhD, Kazuo.

Prevention of Postoperative Pericardial Adhesions With a Novel Regenerative Collagen Sheet  Hiroyuki Tsukihara, MD, Shinichi Takamoto, MD, PhD, Kazuo.
Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 
In Vitro Fabrication of a Tissue Engineered Human Cardiovascular Patch for Future Use in Cardiovascular Surgery  Chao Yang, MD, Ralf Sodian, MD, Ping.

In Vitro Fabrication of a Tissue Engineered Human Cardiovascular Patch for Future Use in Cardiovascular Surgery  Chao Yang, MD, Ralf Sodian, MD, Ping.
Novel Bioengineered Small Caliber Vascular Graft With Excellent One-Month Patency  Yosuke Ishii, MD, Russell T. Kronengold, PhD, Renu Virmani, MD, Elias.

Novel Bioengineered Small Caliber Vascular Graft With Excellent One-Month Patency  Yosuke Ishii, MD, Russell T. Kronengold, PhD, Renu Virmani, MD, Elias.
Tissue Engineering of Autologous Human Heart Valves Using Cryopreserved Vascular Umbilical Cord Cells  Ralf Sodian, MD, Cora Lueders, PhD, Liv Kraemer,

Tissue Engineering of Autologous Human Heart Valves Using Cryopreserved Vascular Umbilical Cord Cells  Ralf Sodian, MD, Cora Lueders, PhD, Liv Kraemer,
Bioengineered vascular access maintains structural integrity in response to arteriovenous flow and repeated needle puncture  Bryan W. Tillman, MD, PhD,

Bioengineered vascular access maintains structural integrity in response to arteriovenous flow and repeated needle puncture  Bryan W. Tillman, MD, PhD,
Biodegradable polymer with collagen microsponge serves as a new bioengineered cardiovascular prosthesis  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Hajime.

Biodegradable polymer with collagen microsponge serves as a new bioengineered cardiovascular prosthesis  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Hajime.
Fariba Chalajour, MD, Laura A

Fariba Chalajour, MD, Laura A
Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential  Lorenzo Boni, MD,

Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential  Lorenzo Boni, MD,
Mesothelium regeneration on acellular bovine pericardia loaded with an angiogenic agent (ginsenoside Rg1) successfully reduces postsurgical pericardial.

Mesothelium regeneration on acellular bovine pericardia loaded with an angiogenic agent (ginsenoside Rg1) successfully reduces postsurgical pericardial.
Biomatrix/Polymer Composite Material for Heart Valve Tissue Engineering  Christof Stamm, MD, Amir Khosravi, MD, Niels Grabow, MS, Kathleen Schmohl, PhD,

Biomatrix/Polymer Composite Material for Heart Valve Tissue Engineering  Christof Stamm, MD, Amir Khosravi, MD, Niels Grabow, MS, Kathleen Schmohl, PhD,
Experimental noninferiority trial of synthetic small-caliber biodegradable versus stable vascular grafts  Damiano Mugnai, MD, Jean-Christophe Tille, MD,

Experimental noninferiority trial of synthetic small-caliber biodegradable versus stable vascular grafts  Damiano Mugnai, MD, Jean-Christophe Tille, MD,
Tissue-engineered stent-graft integrates with aortic wall by recruiting host tissue into graft scaffold  Mugiho Takeuchi, MD, Toru Kuratani, MD, PhD,

Tissue-engineered stent-graft integrates with aortic wall by recruiting host tissue into graft scaffold  Mugiho Takeuchi, MD, Toru Kuratani, MD, PhD,
Bioabsorbable Gelatin Sheets Latticed With Polyglycolic Acid Can Eliminate Pericardial Adhesion  Ichiro Yoshioka, MD, Yoshikatsu Saiki, MD, Kei Sakuma,

Bioabsorbable Gelatin Sheets Latticed With Polyglycolic Acid Can Eliminate Pericardial Adhesion  Ichiro Yoshioka, MD, Yoshikatsu Saiki, MD, Kei Sakuma,
Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 

Preliminary experience with tissue engineering of a venous vascular patch by using bone marrow–derived cells and a hybrid biodegradable polymer scaffold 
Mid-Term Clinical Results Using a Tissue-Engineered Pulmonary Valve to Reconstruct the Right Ventricular Outflow Tract During the Ross Procedure  Pascal.

Mid-Term Clinical Results Using a Tissue-Engineered Pulmonary Valve to Reconstruct the Right Ventricular Outflow Tract During the Ross Procedure  Pascal.
Lucas P. Neff, MD, Bryan W. Tillman, MD, PhD, Saami K

Lucas P. Neff, MD, Bryan W. Tillman, MD, PhD, Saami K
Engineered Living Blood Vessels: Functional Endothelia Generated From Human Umbilical Cord-Derived Progenitors  Dörthe Schmidt, MD, Lars M. Asmis, MD,

Engineered Living Blood Vessels: Functional Endothelia Generated From Human Umbilical Cord-Derived Progenitors  Dörthe Schmidt, MD, Lars M. Asmis, MD,
Mycotic Thoracic Aortic Aneurysm After Intravesical Bacillus Calmette–Guérin Treatment  Kenneth P. Seastedt, MD, Usman Ahmad, MD, Christopher Lau, MD,

Mycotic Thoracic Aortic Aneurysm After Intravesical Bacillus Calmette–Guérin Treatment  Kenneth P. Seastedt, MD, Usman Ahmad, MD, Christopher Lau, MD,
The role of circulating cells in the healing of vascular prostheses

The role of circulating cells in the healing of vascular prostheses

Similar presentations

Ads by Google