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Biodegradable polymer with collagen microsponge serves as a new bioengineered cardiovascular prosthesis  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Hajime.

Published byVíctor Manuel García Acuña Modified over 5 years ago

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Presentation on theme: "Biodegradable polymer with collagen microsponge serves as a new bioengineered cardiovascular prosthesis  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Hajime."— Presentation transcript:

1 Biodegradable polymer with collagen microsponge serves as a new bioengineered cardiovascular prosthesis  Shigemitsu Iwai, MD, Yoshiki Sawa, MD, Hajime Ichikawa, MD, Satoshi Taketani, MD, Eiichiro Uchimura, PhD, Guoping Chen, PhD, Masayuki Hara, PhD, Jun Miyake, PhD, Hikaru Matsuda, MD  The Journal of Thoracic and Cardiovascular Surgery  Volume 128, Issue 3, Pages (September 2004) DOI: /j.jtcvs

2 Figure 1 The PLGA-collagen microsponge patch was 0.6 mm thick (a). Scanning electron microscopy showed the collagen microsponge had a uniformly distributed and interconnected pore structure (pore size of μm; b). The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )

3 Figure 2 Macroscopic findings 6 months after implantation. There was no thrombus formation in any case. The arrows show the border of the patch and the native pulmonary artery. The appearance of the reconstructed portion was not significantly different from that of the native pulmonary artery. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )

4 Figure 3 Microscopic findings of the patch with precellularization after 2 months of implantation showed numerous remaining seeding cells, which were labeled with a fluorescent tracer. (Original magnification 40 ×.) The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )

5 Figure 4 Histologic findings 2 months after implantation. (Original magnification 100 ×.) Hematoxylin-eosin staining (H-E) with (a-1) and without (a-2) precellularization treatment, CD31 staining with (b-1) and without (b-2) precellularization, α-smooth muscle actin staining (A-SMA) with (c-1) and without (c-2) precellularization, and elastica van Gieson staining (EVG) with (d-1) and without (d-2) precellularization are shown. These results show the formation of an endothelial cell monolayer, a parallel alignment of SMCs, and reconstructed vessel wall with elastin and collagen fibers in both groups. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )

6 Figure 5 Immunofluorescence staining of collagen. (Original magnification 100 ×.) Green reveals type I collagen, and red shows type IV collagen. The patch 2 months after implantation without precellularization (a) showed a similar pattern as that seen in the native pulmonary artery (b), with type IV collagen at the basal membrane. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )

7 Figure 6 Hematoxylin-eosin staining (original magnification 40 ×) of the patch 6 months after implantation with (a-1) and without (a-2) precellularization showed a similar architecture to the native pulmonary artery. α-Smooth muscle actin staining (original magnification 40 ×) of the patch 6 months after implantation with (b-1) and without (b-2) precellularization showed widely expanded SMCs in the wall of the patch. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )

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