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Cornelis J. J. Huijsmans, Jeroen Poodt, Paul H. M. Savelkoul, Mirjam H

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Presentation on theme: "Cornelis J. J. Huijsmans, Jeroen Poodt, Paul H. M. Savelkoul, Mirjam H"— Presentation transcript:

1 Sensitive Detection and Quantification of the JAK2V617F Allele by Real-Time PCR 
Cornelis J.J. Huijsmans, Jeroen Poodt, Paul H.M. Savelkoul, Mirjam H.A. Hermans  The Journal of Molecular Diagnostics  Volume 13, Issue 5, Pages (September 2011) DOI: /j.jmoldx Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Standard curves (using twofold dilution steps) generated by JAK2V617F real-time PCR using PNA blocking (squares) and LNA blocking (triangles). Data are given as mean Ct ± SD. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 Amplification plots of JAK2V617F real-time PCR of 25 (A) and 22 (B) healthy individuals in duplicate (JAK2V617F negative cohort) and one 1% JAK2V617F positive control. The amplification plots shown are representative of the entire cohort. Gray horizontal line (at Delta Rn 0.8e-002) indicates threshold; black vertical line, cutoff CT value of 37.9. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

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