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Establishment and Study of Different Real-Time Polymerase Chain Reaction Assays for the Quantification of Cells with Deletions of Chromosome 7  Elia Mattarucchi,

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Presentation on theme: "Establishment and Study of Different Real-Time Polymerase Chain Reaction Assays for the Quantification of Cells with Deletions of Chromosome 7  Elia Mattarucchi,"— Presentation transcript:

1 Establishment and Study of Different Real-Time Polymerase Chain Reaction Assays for the Quantification of Cells with Deletions of Chromosome 7  Elia Mattarucchi, Milena Marsoni, Alberto Passi, Francesco Lo Curto, Francesco Pasquali, Giovanni Porta  The Journal of Molecular Diagnostics  Volume 8, Issue 2, Pages (May 2006) DOI: /jmoldx Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Illustration of the forward primers and the common reverse primer and probe. In square brackets, the MID1064 polymorphism on the partial sequence of 7q35. The Journal of Molecular Diagnostics 2006 8, DOI: ( /jmoldx ) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Plots of the MRD functions (top) and their derivatives (bottom). ΔΔCt values range from 0 to 1 for the Chr versus Chr function and from 0 to for the two alleles function. The Journal of Molecular Diagnostics 2006 8, DOI: ( /jmoldx ) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Plots of Ct values of 100, 20, 2, and 0.2 ng of genomic DNA versus the logarithm of the corresponding DNA amount. For each plot the coefficient of determination (R2), slope, and PCR efficiency are reported. Threshold was set at 0.1. The Journal of Molecular Diagnostics 2006 8, DOI: ( /jmoldx ) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

5 Figure 4 Calculated MRD values. A: The relative difference between nominal and calculated MRD values, SD of measured ΔΔCt), and range of error at the 95% confidence interval are reported for each reference sample. Threshold was set at 0.1. Plots of calculated versus nominal values of MRD are reported in B and C for the Ch versus Ch strategy and the two alleles strategy, respectively. Stippled lines enclose the range of error. Slope and coefficient of determination (R2) of the plots are reported. The Journal of Molecular Diagnostics 2006 8, DOI: ( /jmoldx ) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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