1. The disturbance of TH17-Treg cell balance in adenomyosis
Tao Gui, M.D., Chen Chen, M.D., Zhenzhen Zhang, M.D., Weiwei Tang, M.D., Ruyun Qian, M.D., Xiaoping Ma, M.D., Peng Cao, Ph.D., Guiping Wan, Ph.D. 
Fertility and Sterility 
Volume 101, Issue 2, Pages (February 2014)
DOI: /j.fertnstert
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2. Figure 1
(A) Representative flow cytometry results, showing identification of TH17 and Treg cells in peripheral blood from control women (n = 25), and patients with diffuse (D-AM; n = 29) and focal (F-AM; n = 16) adenomyosis. Analysis of the frequency of (B) TH17 cells, (C) Treg cells, and (D) TH17-Treg ratios in peripheral blood of control women (n = 25) and patients with D-AM (n = 29) and F-AM (n = 16). Dashed lines represent medians, solid lines the lower and upper quartiles. (E) Relative expression of ROR-γt, FOXP3, STAT3 mRNA in control, eutopic, and homologous ectopic endometrium. (F) The histogram shows median concentrations of interleukin (IL) 6, IL-10, IL-17, and transforming growth factor (TGF) β (pg/mL) in the plasma of patients from D-AM (n = 29), F-AM (n = 16), and control (n = 25) groups. Bars and whiskers indicate mean ± SD. Differences between groups were analyzed by Tukey multiple comparision test of one-way analysis of variance (ns = not significant; *P<.05; **P<.01; ***P<.01).
Fertility and Sterility  , DOI: ( /j.fertnstert )
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3. Figure 2
(A) Scatter plot of TH17-Treg ratios and CA-125 levels in women with adenomyosis (AM). The red and blue symbols represent data points from women with diffuse and focal AM, respectively. Pearson rank correlation coefficient was used to analysis their correlations. The dashed lines indicate linear regression fits of the corresponding data. (B) Scatter plot depicting the relationship between the severity of dysmenorrhea in AM and the TH17-Treg ratio in peripheral blood mononuclear cells of patients with AM, in which the solid line represents the lower and upper quartiles and the dashed lines the medians.
Fertility and Sterility  , DOI: ( /j.fertnstert )
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4. Figure 3
(A) Relative expression of FOXP3 and IL-17A mRNA determined by real-time polymerase chain reaction in control endometrium (n = 25), eutopic endometrium (n = 45), and homologous ectopic endometrium (n = 45). (B) Expression of IL-17A+ and FOXP3+ cells in normal endometrium and eutopic and homologous ectopic endometrium. (a) Negative control, using isotype antibodies (matched to IL-17A) staining in normal endometrial tissue. (b, c, d) Immunohistochemical staining of IL-17A in normal, eutopic, and ectopic endometrial samples, respectively. (e) Negative control, using isotype antibodies (matched to FOXP3) staining in adenomyotic lession. (f, g, h) Immunohistochemical staining of FOXP3 in normal, eutopic, and homologous ectopic endometrial samples, respectively. All magnification ×200. Scale bars = 100 μm. Histograms show (C) FOXP3+ and (D) IL-17A+ cell densities per mm2 within the normal, eutopic, and homologous ectopic endometrial samples.
Fertility and Sterility  , DOI: ( /j.fertnstert )
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