1. Sphingosine pathway deregulation in endometriotic tissues
Pietro Santulli, M.D., Louis Marcellin, M.D., Jean-Christophe Noël, M.D., Ph.D., Bruno Borghese, M.D., Ph.D., Isabelle Fayt, M.D., Daniel Vaiman, Ph.D., Charles Chapron, M.D., Céline Méhats, Ph.D. 
Fertility and Sterility 
Volume 97, Issue 4, Pages e5 (April 2012)
DOI: /j.fertnstert
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Real-time polymerase chain reaction (PCR) gene expression analysis of sphingosine-1-phosphate metabolic enzymes in eutopic and ectopic endometrium of 16 different endometriotic women. Depicted is the relative amount of mRNA in endometriotic tissues vs. healthy endometrium. (Left) Baseline levels in endometrium of healthy women. Relative abundance: ++++ = detection between 18 and 25 cycles, very high expression; +++ = 26–28 cycles, high expression; ++ = 29–35 cycles, middle expression; + = 36–37 cycles, low expression; ± = 38–40 cycles, very low expression. The relative expression level was normalized using the geometric mean of three housekeeping genes. Values are mean. Statistical significance: ∗∗P<.01; ∗∗∗P<.001; ∗∗∗∗P<.0001.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Real-time polymerase chain reaction (PCR) gene expression analysis of the five sphingosine-1-phosphate receptors in eutopic and ectopic endometrium from 16 different endometriotic women. Depicted is the relative amount of messenger RNA in endometriotic tissues versus healthy endometrium. (Left) Baseline levels in endometrium of healthy women. Relative abundance: ++++ = detection between 18 and 25 cycles, very high expression; +++ = 26–28 cycles, high expression; ++ = 29–35 cycles, middle expression; + = 36–37 cycles, low expression; ± = 38–40 cycles, very low expression. The relative expression level was normalized using the geometric mean of three housekeeping genes. Values are mean. Statistical significance: ∗P<.05; ∗∗∗P<.001; ∗∗∗∗P<.0001.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
The S1PR2 expression (secretory phase) in normal endometrium of women without endometriosis (A) and with endometriosis (B), and in deep rectovaginal endometriosis (C) and in ovarian endometriosis (D). Note that the protein expression is mainly observed in the cytoplasm of the glandular component.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Simplified scheme of sphingosine-1-phosphate (S1P) metabolism and its modification at the messenger RNA expression levels in eutopic and ectopic endometriotic tissues compared with healthy endometrium; hypothetic model of S1P axis amplification. (A) Baseline levels of messenger RNA expression in healthy endometrium; (B) changes in eutopic endometrium from endometriotic women; (C) changes in ectopic endometrium of endometriotic women compared with healthy tissues. The sizes of the boxes reflect the messenger RNA levels proportion of the different components of the S1P axis.
Fertility and Sterility  , e5DOI: ( /j.fertnstert )
Copyright © 2012 American Society for Reproductive Medicine Terms and Conditions