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Peter R. Nelson, MD, Shinji Yamamura, MD, K.Craig Kent, MD 

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Presentation on theme: "Peter R. Nelson, MD, Shinji Yamamura, MD, K.Craig Kent, MD "— Presentation transcript:

1 Extracellular matrix proteins are potent agonists of human smooth muscle cell migration 
Peter R. Nelson, MD, Shinji Yamamura, MD, K.Craig Kent, MD  Journal of Vascular Surgery  Volume 24, Issue 1, Pages (July 1996) DOI: /S (96) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Concentration-response curves for migration of human SMCs to ECM proteins. Human SMCs were stimulated by ECM proteins in indicated concentrations, and chemokinesis (open box), chemotaxi (Δ), and haptotaxis (open circle) were measured as described in the Material and Methods section. Results are expressed as cells that have migrated + SD. Experiments were performed in triplicate and repeated in at least three cell lines from different donors. Data from a representative experiment is shown. Journal of Vascular Surgery  , 25-33DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Comparison of migratory effects of chemokinesis, chemotaxis, and haptotaxis. Human SMCs were stimulated with ECM proteins in concentrations that provided maximal chemokinesis (CK), chemotaxis (CT), and haptotaxis (HT). Results are expressed as fold-increase in migration versus unstimulated control + SD. Experiments were performed in triplicate and repeated in at least three cell lines from different donors. Data from a representative experiment is shown (p < 0.05, *CK compared with CT; **CT compared with HT). Journal of Vascular Surgery  , 25-33DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 2 Comparison of migratory effects of chemokinesis, chemotaxis, and haptotaxis. Human SMCs were stimulated with ECM proteins in concentrations that provided maximal chemokinesis (CK), chemotaxis (CT), and haptotaxis (HT). Results are expressed as fold-increase in migration versus unstimulated control + SD. Experiments were performed in triplicate and repeated in at least three cell lines from different donors. Data from a representative experiment is shown (p < 0.05, *CK compared with CT; **CT compared with HT). Journal of Vascular Surgery  , 25-33DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 2 Comparison of migratory effects of chemokinesis, chemotaxis, and haptotaxis. Human SMCs were stimulated with ECM proteins in concentrations that provided maximal chemokinesis (CK), chemotaxis (CT), and haptotaxis (HT). Results are expressed as fold-increase in migration versus unstimulated control + SD. Experiments were performed in triplicate and repeated in at least three cell lines from different donors. Data from a representative experiment is shown (p < 0.05, *CK compared with CT; **CT compared with HT). Journal of Vascular Surgery  , 25-33DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 2 Comparison of migratory effects of chemokinesis, chemotaxis, and haptotaxis. Human SMCs were stimulated with ECM proteins in concentrations that provided maximal chemokinesis (CK), chemotaxis (CT), and haptotaxis (HT). Results are expressed as fold-increase in migration versus unstimulated control + SD. Experiments were performed in triplicate and repeated in at least three cell lines from different donors. Data from a representative experiment is shown (p < 0.05, *CK compared with CT; **CT compared with HT). Journal of Vascular Surgery  , 25-33DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7 Fig. 3 Comparison of migratory effects of laminin, fibronectin, and collagen I and IV. Migration of human SMCs exposed to laminin (Lm), fibronectin (Fn), collagen type I (CnI), and collagen type IV (CnIV) with concentrations for each that produced maximal effect. PDGF-AB was also used in comparisons of chemokinesis and chemotaxis. Results are expressed as fold-increase in migration versus unstimulated control + SD. Experiments were performed in triplicate and repeated in at least three cell lines from different donors. Data from a representative experiment is shown (p < 0.05, *PDGF compared with Lm, **Fn compared with PDGF or Lm, # Cn I or IV compared with Fn). Journal of Vascular Surgery  , 25-33DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

8 Fig. 3 Comparison of migratory effects of laminin, fibronectin, and collagen I and IV. Migration of human SMCs exposed to laminin (Lm), fibronectin (Fn), collagen type I (CnI), and collagen type IV (CnIV) with concentrations for each that produced maximal effect. PDGF-AB was also used in comparisons of chemokinesis and chemotaxis. Results are expressed as fold-increase in migration versus unstimulated control + SD. Experiments were performed in triplicate and repeated in at least three cell lines from different donors. Data from a representative experiment is shown (p < 0.05, *PDGF compared with Lm, **Fn compared with PDGF or Lm, # Cn I or IV compared with Fn). Journal of Vascular Surgery  , 25-33DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

9 Fig. 3 Comparison of migratory effects of laminin, fibronectin, and collagen I and IV. Migration of human SMCs exposed to laminin (Lm), fibronectin (Fn), collagen type I (CnI), and collagen type IV (CnIV) with concentrations for each that produced maximal effect. PDGF-AB was also used in comparisons of chemokinesis and chemotaxis. Results are expressed as fold-increase in migration versus unstimulated control + SD. Experiments were performed in triplicate and repeated in at least three cell lines from different donors. Data from a representative experiment is shown (p < 0.05, *PDGF compared with Lm, **Fn compared with PDGF or Lm, # Cn I or IV compared with Fn). Journal of Vascular Surgery  , 25-33DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

10 Fig. 4 Effect of cholera and pertussis toxins on chemo-taxis and haptotaxis. Human SMCs, coincubated with CT (10 μg/ml) or PT (1 μg/ml), were exposed to either chemotactic or haptotactic stimulus of fibronectin (Fn), laminin (Lm), collagen type I (CnI), or collagen type IV (CnIV). Results are expressed as percent of control (vehicle only) chemotaxis/haptotaxis to each of the proteins + SD. Experiments were performed in triplicate and repeated in at least three cell lines from different donors. Data from a representative experiment is shown (p < 0.05, *CT compared with control; **PT compared with control). Journal of Vascular Surgery  , 25-33DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

11 Fig. 4 Effect of cholera and pertussis toxins on chemo-taxis and haptotaxis. Human SMCs, coincubated with CT (10 μg/ml) or PT (1 μg/ml), were exposed to either chemotactic or haptotactic stimulus of fibronectin (Fn), laminin (Lm), collagen type I (CnI), or collagen type IV (CnIV). Results are expressed as percent of control (vehicle only) chemotaxis/haptotaxis to each of the proteins + SD. Experiments were performed in triplicate and repeated in at least three cell lines from different donors. Data from a representative experiment is shown (p < 0.05, *CT compared with control; **PT compared with control). Journal of Vascular Surgery  , 25-33DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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