Presentation is loading. Please wait.

Presentation is loading. Please wait.

Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis 

Published byPosy Hicks Modified over 6 years ago

Similar presentations

Presentation on theme: "Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis "— Presentation transcript:

1 Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis  Alliric I. Willis, MD, Shoichi Fuse, MD, Xiu Jie Wang, MD, Emery Chen, BA, George P. Tuszynski, PhD, Bauer E. Sumpio, MD, PhD, Vivian Gahtan, MD  Journal of Vascular Surgery  Volume 31, Issue 6, Pages (June 2000) DOI: /mva Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Chemotaxis concentration curve for LY Preincubation of vascular smooth muscle cells with the phosphatidylinositol 3-kinase inhibitor LY (10, 50, and 100 μmol/L) demonstrated significant inhibition of thrombospondin-1 (TSP-1 )-, fibronectin (Fn )-, and vitronectin (Vn )-induced chemotaxis at 50 and 100 μmol/L. *P <.05 and **P <.005, compared with their respective uninhibited vascular smooth muscle cell chemotaxis toward TSP-1, Fn, or Vn. A representative experiment is shown. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Attenuation of vascular smooth muscle cell chemotaxis by phosphatidylinositol 3-kinase inhibition. Preincubation of vascular smooth muscle cells with the phosphatidylinositol 3-kinase inhibitor LY (100 μmol/L) significantly attenuated vascular smooth muscle cell chemotaxis toward thrombospondin-1 (TSP-1 ), fibronectin (Fn ), and vitronectin (Vn ), to a level not significantly different from vascular smooth muscle cells exposed to serum-free media (SFM ), P <.05. The chemoattractants TSP-1, Fn, and Vn (20 μg/mL) induced a significant increase in chemotaxis of uninhibited vascular smooth muscle cells, in comparison with SFM (P <.05). LY had no significant effect on the migration of vascular smooth muscle cells exposed to SFM. A representative experiment is shown. Experiments were performed in triplicate and repeated at least three times. *P <.05, compared with their respective uninhibited vascular smooth muscle cell chemotaxis toward TSP-1, Fn, or Vn. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 Chemotaxis concentration curve for GF X. Preincubation of vascular smooth muscle cells with the protein kinase C inhibitor GF X (100 nmol/L, 1 μmol/L, and 5 μmol/L) demonstrated significant inhibition of thrombospondin-1 (TSP-1 )–induced chemotaxis at 1 and 5 μmol/L. *P <.05, compared with their respective uninhibited vascular smooth muscle cells chemotaxis toward TSP-1, Fn, or Vn. A representative experiment is shown. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 4 Attenuation of vascular smooth muscle cell chemotaxis by protein kinase C inhibition. Preincubation of vascular smooth muscle cells with the protein kinase C inhibitor GF X (1 μmol/L) significantly attenuated vascular smooth muscle cell chemotaxis toward thrombospondin-1 (TSP-1 ; P <.05). The chemoattractants TSP-1, fibronectin (Fn ), and vitronectin (Vn ; 20 μg/mL) induced a significant increase in chemotaxis of uninhibited vascular smooth muscle cells in comparison with serum-free media (SFM ; P <.05). GF X had no significant effect on migration of vascular smooth muscle cells exposed to SFM. A representative experiment is shown. Experiments were performed in triplicate and repeated at least three times. *P <.05, compared with their respective uninhibited vascular smooth muscle cell chemotaxis toward TSP-1, Fn, or Vn. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 5 A schematic representation of the potential involvement of phosphatidylinositol 3-kinase (PI3K ) and protein kinase C (PKC ) in thrombospondin-1 (TSP-1 )-, fibronectin (Fn )-, and vitronectin (Vn )-induced vascular smooth muscle cell (VSMC ) chemotaxis. PI3K may serve as an important signal transducer in chemotaxis triggered by the binding of TSP-1, Fn, or Vn. PI3K may relay its signaling via its interaction with G proteins, Ras, or mitogen-activated protein kinase (MAPK ). PKC is likely involved in signal transduction in TSP-1-induced VSMC chemotaxis, but not that of Fn or Vn. Journal of Vascular Surgery  , DOI: ( /mva ) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Download ppt "Inhibition of phosphatidylinositol 3-kinase and protein kinase C attenuates extracellular matrix protein-induced vascular smooth muscle cell chemotaxis "

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,
The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.

The role of arteriovenous shunts in the pathogenesis of varicose veins Howard C. Baron, M.D., Sebastiano Cassaro, M.D. Journal of Vascular Surgery Volume.
The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.

The effect of inguinal lymphatic manipulation on regional lymph flow patterns Jeffrey R. Rubin, MD, Lisa B. Eberlin, MD Journal of Vascular Surgery Volume.
Heparin-coated catheters and heparin-induced thrombocytopenia

Heparin-coated catheters and heparin-induced thrombocytopenia
Peter R. Nelson, MD, Shinji Yamamura, MD, K.Craig Kent, MD 

Peter R. Nelson, MD, Shinji Yamamura, MD, K.Craig Kent, MD 
Axillary-to-carotid artery bypass grafting for symptomatic severe common carotid artery occlusive disease  Joseph P. Archie, PhD, MD  Journal of Vascular.

Axillary-to-carotid artery bypass grafting for symptomatic severe common carotid artery occlusive disease  Joseph P. Archie, PhD, MD  Journal of Vascular.
Heparin inhibits thrombin-induced mitogen-activated protein kinase signaling in arterial smooth muscle cells  Ulf Hedin, MD, PhD, Günter Daum, PhD, Alexander.

Heparin inhibits thrombin-induced mitogen-activated protein kinase signaling in arterial smooth muscle cells  Ulf Hedin, MD, PhD, Günter Daum, PhD, Alexander.
Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway  Hidenori.

Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway  Hidenori.
Mitogen-activated protein kinase activation: An alternate signaling pathway for sustained vascular smooth muscle contraction  Aaron M. Epstein, MD, Douglas.

Mitogen-activated protein kinase activation: An alternate signaling pathway for sustained vascular smooth muscle contraction  Aaron M. Epstein, MD, Douglas.
Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α  Alan Dardik,

Shear stress-stimulated endothelial cells induce smooth muscle cell chemotaxis via platelet-derived growth factor-BB and interleukin-1α  Alan Dardik,
Kristopher G. Maier, PhD, Xuan Han, Benjamin Sadowitz, MD, Karen L

Kristopher G. Maier, PhD, Xuan Han, Benjamin Sadowitz, MD, Karen L
Urokinase-induced smooth muscle cell responses require distinct signaling pathways: A role for the epidermal growth factor receptor  Suzanne M. Nicholl,

Urokinase-induced smooth muscle cell responses require distinct signaling pathways: A role for the epidermal growth factor receptor  Suzanne M. Nicholl,
Heparin, urokinase, and ancrod alter neutrophil function

Heparin, urokinase, and ancrod alter neutrophil function
Peter R. Nelson, MD, Shinji Yamamura, MD, K.Craig Kent, MD 

Peter R. Nelson, MD, Shinji Yamamura, MD, K.Craig Kent, MD 
The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro  Carol J. Sawmiller, MD, Richard J. Powell, MD,

The differential effect of contrast agents on endothelial cell and smooth muscle cell growth in vitro  Carol J. Sawmiller, MD, Richard J. Powell, MD,
Kinase activation and smooth muscle contraction in the presence and absence of calcium  Glenn Whitney, MD, Doug Throckmorton, MD, Carlos Isales, MD, Yo.

Kinase activation and smooth muscle contraction in the presence and absence of calcium  Glenn Whitney, MD, Doug Throckmorton, MD, Carlos Isales, MD, Yo.
A new valvulotome for in situ bypass grafts

A new valvulotome for in situ bypass grafts
Nicholas P. Ziats, PhD, James M. Anderson, MD, PhD 

Nicholas P. Ziats, PhD, James M. Anderson, MD, PhD 
Hiroyuki Itoh, MD, Peter R

Hiroyuki Itoh, MD, Peter R
Omar Araim, MD, James Ballantyne, Andrew L. Waterhouse, PhD, Bauer E

Omar Araim, MD, James Ballantyne, Andrew L. Waterhouse, PhD, Bauer E

Similar presentations

Ads by Google