1. Cell selection by selective matrix adhesion is not sufficiently efficient for complete malignant cell depletion from contaminated human testicular cell suspensions 
Mieke Geens, Ph.D., Ellen Goossens, Ph.D., Herman Tournaye, M.D., Ph.D. 
Fertility and Sterility 
Volume 95, Issue 2, Pages (February 2011)
DOI: /j.fertnstert
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Overview of the matrix adhesion-based selection procedure. Testicular cell suspensions were cultured on uncoated dishes and subjected to magnetic-activated cell sorting (MACS) for CD49f expression and incubation on collagen I and laminin-coated culture plates. Finally selected cells were analyzed by fluorescence-activated cell sorting (FACS) and polymerase chain reaction (PCR) to determine the presence of residual contaminating cells. Samples of the initial cell suspensions, as well as all cells that had to be discarded during the selection procedure, were analyzed as control subjects by PCR.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Analysis of the cell suspensions during and after selection. (A) Analysis of the polymerase chain reaction (PCR) fragments for the final selected cell suspensions in all five patients, as well as for positive (SB cells) and negative (H2O) control samples. In all initially contaminated sorted cell suspensions, a peak was observed corresponding with a 120-bp PCR fragment specific for the SB cell line. All uncontaminated control samples were negative for this fragment. In patient 4, another peak, not corresponding to the SB cell line, was observed, possibly derived from a patient-specific residual B-cell. (B–D) FACS analysis for expression of human leukocyte antigen (HLA) class I and CD49f in SB cells (B) and finally selected uncontaminated (C) and contaminated (D) cells of patient 1. HLA class I proteins are highly expressed, whereas CD49f cannot be detected in the in vitro–cultured SB cell line (B). No HLA class I+ CD49f− cells could be detected after the complete selection procedure in uncontaminated testicular cells of patient 1 (C), whereas 2.1% HLA class I+ CD49f− cells were present after the same selection procedure with contaminated testicular cells of the same patient (D).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2011 American Society for Reproductive Medicine Terms and Conditions