Presentation is loading. Please wait.

Presentation is loading. Please wait.

Wanfen Xiong, PhD, Rebecca A. Knispel, BS, Harry C

Published byDaniela Kohler Modified over 5 years ago

Similar presentations

Presentation on theme: "Wanfen Xiong, PhD, Rebecca A. Knispel, BS, Harry C"— Presentation transcript:

1 Doxycycline delays aneurysm rupture in a mouse model of Marfan syndrome 
Wanfen Xiong, PhD, Rebecca A. Knispel, BS, Harry C. Dietz, MD, Francesco Ramirez, PhD, B. Timothy Baxter, MD  Journal of Vascular Surgery  Volume 47, Issue 1, Pages (January 2008) DOI: /j.jvs Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 The effect of doxycycline on the survival of mgR/mgR mice. Mice were treated with doxycycline (doxy) (100 mg/kg) or regular water (no doxy) daily, started at the age of 1 day. Life table analysis shows improved survival in mice treated with doxycycline (P = .0009). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Gelatin zymographic analysis of MMP-2 and MMP-9 in the mouse thoracic aortas. Mouse thoracic aortas from wild type control, mgR/mgR, and doxycycline-treated mgR/mgR mice harvested at 6 weeks of age (n = 5 mice/group). Aortic proteins were extracted and separated by electrophoresis on a 10% SDS-PAGE containing 0.8% gelatin. Band intensities (gelatinolytic activities) were measured by densitometer. Relative expression of MMP-9 and MMP-2 in wild type control, mgR/mgR, and doxycycline-treated mgR/mgR mouse aortas was analyzed. *, # P < 0.05 (*relative to wild type controls, # relative to untreated mgR/mgR mice). A representative gel is shown. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 Reverse zymographic analysis of TIMP levels in the mouse thoracic aortas. Mouse thoracic aortas from wild type control, mgR/mgR, and doxycycline-treated mgR/mgR mice harvested at 6 weeks of age (n = 5 mice/group). Aortic proteins were separated by electrophoresis on a 12.5% SDS-PAGE containing 1.6% gelatin, 0.16 μg/ml MMP-2. A representative reverse zymogram shows the bands representing gelatin preserved by TIMP-1 and TIMP-2 (indicated by arrows). Band intensities were measured by densitometer. Relative expression of TIMP-1 and TIMP-2 in wild type control, mgR/mgR, and doxycycline-treated mgR/mgR mouse aortas was analyzed. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 Elastic fiber degradation in the wild type (WT) and mgR/mgR mice. a, elastin breaks per field under 40x magnification in WT, mgR/mgR, and doxycycline-treated mgR/mgR mice (n = 3 aortas/group). *, # P < 0.05 (*relative to wild type controls, # relative to untreated mgR/mgR mice). b, c, and d, representative trichrome stainings of aortic tissue of WT (b), mgR/mgR (c), and doxycycline-treated mgR/mgR (d) mice at 6 weeks age. Arrows indicates breaks in elastic fibers. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

Download ppt "Wanfen Xiong, PhD, Rebecca A. Knispel, BS, Harry C"

Similar presentations

Effectiveness of combination of losartan potassium and doxycycline versus single-drug treatments in the secondary prevention of thoracic aortic aneurysm.

Effectiveness of combination of losartan potassium and doxycycline versus single-drug treatments in the secondary prevention of thoracic aortic aneurysm.
Analysis of motor and somatosensory evoked potentials during thoracic and thoracoabdominal aortic aneurysm repair  Kourosh Keyhani, DO, Charles C. Miller,

Analysis of motor and somatosensory evoked potentials during thoracic and thoracoabdominal aortic aneurysm repair  Kourosh Keyhani, DO, Charles C. Miller,
Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,

Mesenchymal stem cells attenuate angiotensin II-induced aortic aneurysm growth in apolipoprotein E-deficient mice  Ryotaro Hashizume, MD, Aika Yamawaki-Ogata,
Regulation of vascular smooth muscle cell expression and function of matrix metalloproteinases is mediated by estrogen and progesterone exposure  Oscar.

Regulation of vascular smooth muscle cell expression and function of matrix metalloproteinases is mediated by estrogen and progesterone exposure  Oscar.
Castration of male mice prevents the progression of established angiotensin II-induced abdominal aortic aneurysms  Xuan Zhang, PhD, Sean Thatcher, PhD,

Castration of male mice prevents the progression of established angiotensin II-induced abdominal aortic aneurysms  Xuan Zhang, PhD, Sean Thatcher, PhD,
Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats 

Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats 
Endogenous superoxide dismutase activation by oral administration of riboflavin reduces abdominal aortic aneurysm formation in rats  Zhenhai Yu, MD, Keisuke.

Endogenous superoxide dismutase activation by oral administration of riboflavin reduces abdominal aortic aneurysm formation in rats  Zhenhai Yu, MD, Keisuke.
Hyperglycemia limits experimental aortic aneurysm progression

Hyperglycemia limits experimental aortic aneurysm progression
Mark J.W. Koelemay, MD, PhD  Journal of Vascular Surgery 

Mark J.W. Koelemay, MD, PhD  Journal of Vascular Surgery 
Coarctation-induced degenerative abdominal aortic aneurysm in a porcine model  Pao-Yen Lin, MD, Yeng-Ting Wu, MS, Guan-Cheng Lin, BS, Yao Hsiang Shih,

Coarctation-induced degenerative abdominal aortic aneurysm in a porcine model  Pao-Yen Lin, MD, Yeng-Ting Wu, MS, Guan-Cheng Lin, BS, Yao Hsiang Shih,
Transglutaminase type 2 in human abdominal aortic aneurysm is a potential factor in the stabilization of extracellular matrix  Sung Shin, MD, Yong-Pil.

Transglutaminase type 2 in human abdominal aortic aneurysm is a potential factor in the stabilization of extracellular matrix  Sung Shin, MD, Yong-Pil.
Age-related trends in utilization and outcome of open and endovascular repair for abdominal aortic aneurysm in the United States, 2001-2006  Margaret.

Age-related trends in utilization and outcome of open and endovascular repair for abdominal aortic aneurysm in the United States,   Margaret.
Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.
Loss of STAT1 is associated with increased aortic rupture in an experimental model of aortic dissection and aneurysm formation  Matthew J. Eagleton, MD,

Loss of STAT1 is associated with increased aortic rupture in an experimental model of aortic dissection and aneurysm formation  Matthew J. Eagleton, MD,
Tamoxifen up-regulates catalase production, inhibits vessel wall neutrophil infiltration, and attenuates development of experimental abdominal aortic.

Tamoxifen up-regulates catalase production, inhibits vessel wall neutrophil infiltration, and attenuates development of experimental abdominal aortic.
Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.

Effect of blocking platelet activation with AZD6140 on development of abdominal aortic aneurysm in a rat aneurysmal model  Jianping Dai, MD, PhD, Liliane.
Doxycycline therapy for abdominal aneurysm: Improved proteolytic balance through reduced neutrophil content  Hazem Abdul-Hussien, MD, Roeland Hanemaaijer,

Doxycycline therapy for abdominal aneurysm: Improved proteolytic balance through reduced neutrophil content  Hazem Abdul-Hussien, MD, Roeland Hanemaaijer,
Matrix metalloproteinase inhibition reduces intimal hyperplasia in a porcine arteriovenous-graft model  Joris I Rotmans, MD, Evelyn Velema, BSc, Hence.

Matrix metalloproteinase inhibition reduces intimal hyperplasia in a porcine arteriovenous-graft model  Joris I Rotmans, MD, Evelyn Velema, BSc, Hence.
The fibrillin-1 hypomorphic mgR/mgR murine model of Marfan syndrome shows severe elastolysis in all segments of the aorta  Simon Schwill, Philipp Seppelt,

The fibrillin-1 hypomorphic mgR/mgR murine model of Marfan syndrome shows severe elastolysis in all segments of the aorta  Simon Schwill, Philipp Seppelt,
Controlled release of ascorbic acid from gelatin hydrogel attenuates abdominal aortic aneurysm formation in rat experimental abdominal aortic aneurysm.

Controlled release of ascorbic acid from gelatin hydrogel attenuates abdominal aortic aneurysm formation in rat experimental abdominal aortic aneurysm.

Similar presentations

Ads by Google