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Progesterone regulation of glutathione S-transferase Mu2 expression in mouse uterine luminal epithelium during preimplantation period  Hua Ni, Ph.D.,

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Presentation on theme: "Progesterone regulation of glutathione S-transferase Mu2 expression in mouse uterine luminal epithelium during preimplantation period  Hua Ni, Ph.D.,"— Presentation transcript:

1 Progesterone regulation of glutathione S-transferase Mu2 expression in mouse uterine luminal epithelium during preimplantation period  Hua Ni, Ph.D., Xing-Jiang Yu, B.S., Hua-Jie Liu, B.S., Wei Lei, B.S., Deivendran Rengaraj, Ph.D., Xiao-Juan Li, Ph.D., Zeng-Ming Yang, Ph.D.  Fertility and Sterility  Volume 91, Issue 5, Pages (May 2009) DOI: /j.fertnstert Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Gstm2 expression during early pregnancy. (A) In situ hybridization of Gstm2 expression in mouse uteri during early pregnancy on days 1, 2, 3, 4, and 5 (D1–D5; D5-I = implantation site; D5-N = interimplantation site). Bar = 100 μm. (B) Representative photograph of reverse-transcription polymerase chain reaction of Gstm2 expression in mouse uterus on days 1–8 during pregnancy. (C) Ratio between Gstm2 and Gapdh. Gstm2 expression was normalized to Gapdh. Each bar shows mean ± SEM of samples. Bars with different letters at the top differ significantly. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Gstm2 expression during pseudopregnancy. (A) In situ hybridization of Gstm2 expression in mouse uteri during pseudopregnancy on days 1, 3, 4, and 5 (PD1–PD5). Bar = 100 μm. (B) Representative photograph of reverse-transcription polymerase chain reaction. (C) Ratio between Gstm2 and Gapdh. Each bar shows mean ± SEM of samples. Bars with different letters at the top differ significantly. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 In situ hybridization of Gstm2 expression under delayed implantation, and 12 and 24 h after activation by estrogen treatment. ∗ = blastocyst. Bar = 80 μm. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

5 Figure 4 Progesterone regulation of Gstm2 expression. (A) In situ hybridization of Gstm2 expression in ovariectomized mouse uteri after progesterone treatments for 0 (control), 3, 6, 9, 12, and 24 h. Bar = 100 μm. (B, E) Representative photographs of reverse-transcription polymerase chain reaction. (C, F) Ratio between Gstm2 and Gapdh. Each bar shows mean ± SEM of samples. Bars with different letters at the top differ significantly. (D) In situ hybridization of Gstm2 expression in mouse uteri on day 4 of pregnancy after pregnant mice on day 3 were treated with 0.4 mg RU486 in 0.1 mL sesame oil or sesame oil (Control) for 24 h. Bar = 100 μm. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions


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