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Natural latex, grass pollen, and weed pollen share IgE epitopes

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Presentation on theme: "Natural latex, grass pollen, and weed pollen share IgE epitopes"— Presentation transcript:

1 Natural latex, grass pollen, and weed pollen share IgE epitopes
Thomas Fuchs, MD a, Susanne Spitzauer, MD b, Claudia Vente, MD a, Joachim Hevler c, Sonja Kapiotis b, Helmut Rumpold, MD b, Dietrich Kraft, MD d, Rudolph Valenta, MD d  Journal of Allergy and Clinical Immunology  Volume 100, Issue 3, Pages (September 1997) DOI: /S (97) Copyright © 1997 Mosby, Inc. Terms and Conditions

2 Fig. 2 Inhibition of IgE binding to milk proteins by rBet v 1, rBet v 2, mugwort, and ragweed extract. Sera from nine individuals with latex allergy (numbered as in Table 1) were preincubated with bovine serum albumin (lane B), rBet v 1 (B1), rBet v 2 (B2) , ragweed pollen extract (R), or mugwort pollen extract (M), before probing for IgE reactivity with nitrocellulose-blotted latex milk proteins. Lane N represents a control with the serum from a nonallergic individual, and lane P represents the buffer control without addition of serum. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

3 Fig. 2 Inhibition of IgE binding to milk proteins by rBet v 1, rBet v 2, mugwort, and ragweed extract. Sera from nine individuals with latex allergy (numbered as in Table 1) were preincubated with bovine serum albumin (lane B), rBet v 1 (B1), rBet v 2 (B2) , ragweed pollen extract (R), or mugwort pollen extract (M), before probing for IgE reactivity with nitrocellulose-blotted latex milk proteins. Lane N represents a control with the serum from a nonallergic individual, and lane P represents the buffer control without addition of serum. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

4 Fig. 3 Inhibition of IgE binding to latex milk with timothy grass pollen extract. Sera from six individuals with latex allergy (numbered as in Table I) were preincubated with bovine serum albumin or timothy grass pollen extract (lane +) before probing with nitrocellulose-blotted latex milk extract. Lane N represents a control with serum from a nonallergic individual, and lane P represents the buffer control without addition of serum. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

5 Fig. 4 Influence of periodate treatment on IgE binding to nitrocellulose-blotted latex milk extract and latex glove extract. Latex milk extract (M) and latex glove extract (G) were separated by SDS-PAGE and blotted onto nitrocellulose. Strips were preincubated with periodate (p) or sodium acetate buffer before incubation with sera from patients with latex allergy (numbered as in Table I), serum from an individual with mugwort allergy (M), or buffer (B). Bound IgE was detected with 125I-labeled anti-human IgE antibodies. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

6 Fig. 4 Influence of periodate treatment on IgE binding to nitrocellulose-blotted latex milk extract and latex glove extract. Latex milk extract (M) and latex glove extract (G) were separated by SDS-PAGE and blotted onto nitrocellulose. Strips were preincubated with periodate (p) or sodium acetate buffer before incubation with sera from patients with latex allergy (numbered as in Table I), serum from an individual with mugwort allergy (M), or buffer (B). Bound IgE was detected with 125I-labeled anti-human IgE antibodies. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

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