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Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: Seed storage proteins as common food allergens  Kirsten Beyer,

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Presentation on theme: "Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: Seed storage proteins as common food allergens  Kirsten Beyer,"— Presentation transcript:

1 Identification of sesame seed allergens by 2-dimensional proteomics and Edman sequencing: Seed storage proteins as common food allergens  Kirsten Beyer, MD, Ludmilla Bardina, MS, Galina Grishina, MS, Hugh A. Sampson, MD  Journal of Allergy and Clinical Immunology  Volume 110, Issue 1, Pages (July 2002) DOI: /mai Copyright © 2002 Mosby, Inc. Terms and Conditions

2 Fig. 1 SDS-PAGE analysis for sesame seed proteins: lane 2, labeling with Coomassie blue stain for total protein analysis; lanes 4 to 23, immunolabeling with sera from 20 patients with sesame seed allergy; lane 24, labeling with control sera. Molecular weight standards (MW) are shown in lanes 1, 3, and 25 . Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2002 Mosby, Inc. Terms and Conditions

3 Fig. 2 Two-dimensional proteomics map for sesame seed proteins. Shown is labeling with Coomassie blue for total protein analysis and immunolabeling of 4 representative patients and the control serum. Molecular weight standards (MW) are shown at left . Red circles indicate proteins recognized at 7, 34, 45, and 78 kd, for which Edman sequencing was performed. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2002 Mosby, Inc. Terms and Conditions

4 Fig. 3 Shown is the homology in amino acid sequences for the sesame seed 7S globulin and Ara h 1 in the area of an IgE-binding epitope of Ara h 1. Identical amino acids are shown in bold and underlined, and homologous amino acids are underlined only. Amino acids highlighted in gray are known to lead to a loss of IgE binding when altered.14 Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2002 Mosby, Inc. Terms and Conditions

5 Fig. 4 Shown are the amino acid sequences for the large subunits of the two 2S albumins of sesame seed. Identical amino acids are shown in bold and underlined, and homologous amino acids are underlined only. The two 2S albumins of sesame seed are, in general, only 35% identical and 47% homologous. Amino acids highlighted in light gray were found to be the sequence of an internal fragment of the 7-kd allergen in the present study, and amino acids are highlighted in dark gray in the sequence of an internal fragment of the allergen in the study by Pastorello et al.13 Adapted from Pastorello et al.13 Copyright 2001 with permission from Elsevier Science. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2002 Mosby, Inc. Terms and Conditions

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