1. Volume 55, Issue 6, Pages 2299-2309 (June 1999)
Combined administration of IgA and IgG anti-Thy-1 antibodies enhances renal inflammation in rats 
Mieneke G.A. Van Dixhoorn, Toshinobu Sato, Yvonne Muizert, Danielle J. Van Gijlswijk-Janssen, Emile de Heer, Mohamed R. Daha 
Kidney International 
Volume 55, Issue 6, Pages (June 1999)
DOI: /j x
Copyright © 1999 International Society of Nephrology Terms and Conditions

2. Figure 1
Albuminuria in rats injected with increasing amounts of ER4G on days 1 (□) and 7 (▪) (depicted are averages ± SD of N = 3 per dose).
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

3. Figure 2
Immunofluorescence staining using FITC-conjugated goat antimouse IgG antibodies in glomeruli of rats injected with PBS, (A) a limiting dose of ER4G (0.4mg/kg; B) or an optimal dose of ER4G (1.0mg/kg; C) on day 1 after injection (all, ×250).
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

4. Figure 3
Immunofluorescence staining using FITC-conjugated goat antimouse IgA antibodies in kidney cross-sections of rats injected with PBS (A) or 7.5mg/kg ER4A (B) on day 1 after injection (all, ×250).
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

5. Figure 4
Immunofluorescence staining using a mouse monoclonal antibody directed against rat C6 on kidney cross-sections of rats injected with PBS (A), a limiting dose of ER4G (B), ER4A (C), or a combination of a limiting dose of ER4G in the presence of ER4A (D) on day 1 after the injection (all, ×250). Rat C6 was only detected in glomeruli of rats treated with the combination of anti-Thy-1 antibodies.
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

6. Figure 5
Immunofluorescence staining using a mouse monoclonal antibody directed against rat C5b-9 on kidney cross-sections of rats injected with PBS (A), a limiting dose of ER4G (B), ER4A (C), or a combination of a limiting dose of ER4G in the presence of ER4A (D) on day 1 after the injection (all, ×250). Rat C5b-9 was only observed in glomeruli of rats treated with the combination of ER4 monoclonal isotypes.
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

7. Figure 6
Assessment of albuminuria as determined by rat serum albumin (RSA) excretion in urine of rats injected with ER4 monoclonal antibodies seven days after antibody injection. A significant (P < 0.02; indicated by the asterisk), synergistic increase in albuminuria was found in the combination group as compared with groups of rats injected with a single antibody or PBS alone. Depicted are averages ± sd of six rats per group.
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

8. Figure 7
Light microscopy of rat kidney cross-sections of day 7 after administration of PBS (A), ER4G alone (B), ER4A alone (C), or the combination of ER4A together with ER4G (D; methenamine silver staining, ×250). Mesangial cell proliferation and ECM expansion are indicated by small arrowheads. Glomerular adhesion to Bowman's capsule is indicated by the larger arrowhead.
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions

9. Figure 8
Average extracellular matrix (ECM) score (± SEM) in PAS-stained kidney biopsies (20 glomeruli per biopsy) of rats injected with PBS, ER4A, ER4G, or the combination of antibodies on day 7. The group of rats receiving the combination of anti-Thy-1 antibodies revealed a significant (P < 0.05) additive increased ECM as compared with rats receiving PBS or single antibody injections alone (indicated by asterisk). Depicted are averages ± sd of six rats per group.
Kidney International  , DOI: ( /j x)
Copyright © 1999 International Society of Nephrology Terms and Conditions