1. Altered microRNA expression profiles of human spermatozoa in patients with different spermatogenic impairments 
Masood Abu-Halima, M.Sc., Mohamad Hammadeh, Prof. Dr., Jana Schmitt, M.Sc., Petra Leidinger, Ph.D., Andreas Keller, Ph.D., Eckart Meese, Prof. Dr., Christina Backes, Ph.D. 
Fertility and Sterility 
Volume 99, Issue 5, Pages e16 (April 2013)
DOI: /j.fertnstert
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Clustering of 50 miRNAs with the highest variances. A = asthenozoospermic samples; OA = oligoasthenozoospermic samples; N = normozoospermic samples. Complete linkage hierarchic clustering was performed with the Euclidian distance measure. Subfertile patients and fertile healthy control subjects cluster separately with the exception of three samples (A-4, A-6, and N-7). The colors in the heatmap represent normalized values, with lower values being colored in shades of green and higher values in shades of red.
Fertility and Sterility  , e16DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Verification of miRNA level by quantitative real-time polymerase chain reaction of six differentially expressed miRNAs in asthenozoospermia (A) and oligoasthenozoospermia (OA) versus normospermia (N) (>2.0-fold difference and 5% false-discovery rate). miRNAs are listed on the x-axis, and the y-axis refers to the relative expression levels.
Fertility and Sterility  , e16DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

4. Supplemental Figure 1
Detection of argonaute-2/eukaryotic translation initiation factor 2C 2. Agarose gel showing the polymerase chain reaction (PCR) products of semen samples which were prepared individually by Puresperm density-gradient purification. Spermatozoa, whole ejaculate, and HeLa cell type (positive control). A representative photograph is shown for randomly selected samples from our tested groups. Highly purified spermatozoa were obtained from the Puresperm fractions (lanes 1 and 2), whole ejaculate from the same patient sample (lane 3), and HeLa cell type (lane 4); lanes 5–8 are reverse transcription control, and lane 9 PCR negative control. M = DNA ladder (100 bp).
Fertility and Sterility  , e16DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

5. Supplemental Figure 2
A Venn diagram showing the overlap of miRNAs that were found to be down-regulated and up-regulated in asthenozoospermic (A) and oligoasthenozoospermic (OA) compared with normozoospermic (N) sample groups. Venn diagram created with the use of Venny (
Fertility and Sterility  , e16DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

6. Supplemental Figure 3
Up-regulated and down-regulated miRNAs in asthenozoospermic (A) and oligoasthenozoospermic (OA) compared with normozoospermic (N) sample groups. Venn diagrams show miRNA expressions that were (A) increased 2-fold or (B) decreased 2-fold. Listed are the miRNAs that overlapped between these two different data sets. Venn diagram created with the use of Venny (
Fertility and Sterility  , e16DOI: ( /j.fertnstert )
Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions