3BACTERIA AS A PATHOGENBacteria are unicellular organisms that reproduce by cell division; usually have cell walls; can be shaped like spheres, rods or spirals; and can be found in virtually any environment.Although many are synergistic in organisms, they can also cause disease. In veterinary medicine we routinely see them affecting nine areas:Outer Ear InfectionsUrinary Tract InfectionsConjunctival infectionsGenital infectionsWound/abscessSkin InfectionsUpper Respiratory InfectionsMastitisSepticemia
4CURRENT METHODS OF DETECTION AND IDENTIFICATION There are generally two steps involved in the detection,identification and treatment of bacterial pathogensCultureGrowing suspected bacterial agents in a nutrientmedia (agar) within a controlled environment(incubator)SensitivitySubjecting bacterial agents to various commonveterinary antibiotics to determine efficacy.
5CURRENT METHODS OF DETECTION AND IDENTIFICATION (continued) Most veterinary clinics and hospitals for lack of a comprehensive,easy to use in-house method opt for using a reference laboratory.This involves:Collecting a sample from the affected areaSending it to a reference laboratoryTreat suspected bacteria with antibioticReceiving the culture and/or sensitivity results in 2-3 daysRecheck in 10 days to adjust antibiotic if necessary.This comes at a great cost per test to both the veterinarian and theclient with no guarantee the sample will not be deemed unusable.(Note: Up to 30% of all UTI samples sent to labs are rejected.)
6Veterinary Microbiology Culture System MultiChromeTMVeterinary Microbiology Culture SystemMultiChrome GP(+)Exp 3/10Lot #123MultiChrome GP(-)MultiChrome is cutting edge technology for Veterinarymicrobiology. At the center of this system is a uniqueChromogenic culture bi-plate that:Isolates Gram Positive from Gram Negative bacteriaDifferentiation of bacteria into two families based on their abilityto accept or reject a chromogen indicator.Gram Positive(+)(-)Gram NegativeMultiChrome GN(-)MultiChrome GP(+)Exp 3/10Lot #123E. faecalis (Bone)S. intermedius (Ear)S. aureus (Skin)S. uberis (Mastitis)Proteus (UTI)Pseudomonas (Ear)Klebsiella (UTI)E. Coli (UTI 90%)
7Is designed to ignite, individual, distinct color within bacteria Causes a reaction within the bacteriaresulting in distinct colors such as inE. Coli appearing as a rose coloredcolony with a darker center orStrep Agalactiae appearing teal toturquoise blue, etc.
8Bacterial pathogen probability charts for Canine and Feline: These reference charts suggest the highest and lowest percentage for infectionof various parts of the body by common pathogenic bacteria. Once identifiedproceed with accepted veterinary antibiotic protocols for treatment of pathogens.Species: CaninePercentageProbabilityBacteriaUTIEarBoneSkinGenitalWoundBloodURIStaph Intermedius10-15%25-30%40-50%60-70%15-25%25-35%30-35%E. Coli>50%10-20%20-30%35-45%15-20%Enterococcus faecalis<10%NGSProteus mirabilis15%20-25%Obligate anerobesPseudomonasKlebsiella20-35%Pasturella10-25%NGS= Not Generally SeenSpecies: FelinePercentProbabilityBacteriaConjunctivalGenitalUTIWoundURISkinPasturella10-20%15-25%10-15%30-40%>50%E. ColiNGS30-35%40-45%15-20%20-30%Proteus mirabilis<10%PseudomonasObligate anerobes10-25%25-35%KlebsiellaChlamydia psittoci50-75%B-hemolytic strepNGS= Not Generally SeenProbability reference charts taken from “Target: The Antimicrobial Reference Guide to Effective Treatment” , second edition, revised.Author: Dr. David Aucoin, DVM, Diplomate of the American College of Veterinary Clinical Pharmacology. Data on file, Kacey® Asheville, N.C
9Veterinary Microbiology Culture System MultiChromeTMVeterinary Microbiology Culture SystemProduces in-house confirmatory results in 24 hoursNo waiting for lab results that can take up to 3 daysand STILL be deemed inconclusive or unusable.Why wait for results?
10“MultiChrome™ Microbiology System In-House Culture ProcedureMaterials needed:A sterile Swab or inoculating loop (10ul recommended)One MultiChrome plate brought to room temperature (stored in fridge)Existing incubator or the NEW MultiChrome™ Micro IncubatorMultiChrome GN(-)MultiChrome GP(+)Exp 3/10Lot #123
11“MultiChrome™ Microbiology System In-House Culture Procedure (continued)Procedure: Simple, Fast & Easy!Obtain sample from affected area with a swab or loopStreak both sides of the MultiChrome plate in a zigzag fashionPlace in pre heated incubator (98.6 F or 37 C)Read in 24 hoursExp 3/10Lot #123Exp 3/10Lot #123MultiChrome GP(+)MultiChrome GN(-)MultiChrome GP(+)MultiChrome GN(-)Inoculated24 Hour Confirmation
12“MultiChrome™ Microbiology System In-House Culture Procedure (continued)Reading Results:Confirmation can be madefirst by its +/- Gram familyand then by its distinctivecolor match to the referencechart included in each packof MultiChrome™.Veterinarians can treat at thisstage with the antibiotics mostappropriate for the bacteriafound. A re-check in ten daysis recommendedConfirmation and Identificationin just 24 hours!Gram PositiveGram NegativeS. Aureus*S. IntermediusE. faecalisE. ColiS. saprophyticusKlebsiellaPseudomonasOnly a few of the bacteria detected!*Can even detect super bug MRSA with the MultiChrome™ MRSA specificTest plate coming in 12/09. MRSA (Methicillin Resistant Staph Aureus) hasbeen recently identified on companion animals and is a zoonotic.
13“MultiChrome™ Microbiology System and Sensitivity TestingWe have now completed the first stage in a microbiology in-housesystem and we could stop there, but what about Sensitivity testing?Simply inoculate a “Mueller Hinton” agar plate with confirmed growthfrom the MultiChrome™ plate, drop in antibiotic test discs and incubatefor another 24 hours.Kacey “MultiChrome™Micro IncubatorSensitivity disk withantibiotic discs
14Kacey MultiChrome™ Micro Incubator Although the MultiChrome™ system can be used with conventionalincubators the patented Kacey Micro Incubator is far from conventional.Here is why:Small footprint (8” wide, 13” high, 12” deep)Precision temperature controlDigital temperature readoutSliding trays which cradle for all forms of dishesClear door for observationTwelve individual 24 hour countdown timersAudible and visible alert when sample is readyLightweight ABS construction, 1 year warrantySuitable for MultiChrome, Dermatophyte, Sensitivity andrelated diagnostics requiring incubationEasy Operation:1- Load Sample2- Press timer3- Walk away
15MultiChrome™ Microbiology System Benefits of using theMultiChrome™ Microbiology SystemTo best demonstrate this, let’s compare the Diagnostic andEconomic benefits of MultiChrome™ versus the Ref. Lab.Diagnostics:BenefitsMultiChrome™Ref LaboratoryAccuracyWithin 5-10%*Within 5-10%Turn around time24 hours2-3 daysFlexibilityCulture and/or sensitivityNo stand aloneSensitivities accepted*Well within the acceptable error range of most labs. Accuracy will improve with practice.For unresponsive bacterial agents and/or unknown etiology we do recommend the use ofa Reference lab for more advanced diagnostic tests.
16MultiChrome™ Microbiology System (continued) Benefits of using theMultiChrome™ Microbiology System (continued)Economic benefit to the Veterinarian: It’s all about gross profit! Let’s compare the current reference lab prices to the MultiChrome™ systemon one Culture & Sensitivity sample.Reference Labs$58.00 (avg.) Cost for C&S$ (avg.) Client fee (2x lab fee)$58.00 Gross ProfitMultiChrome™$8.00 (avg.) Cost for Culture$ (avg.) Client fee$ Gross ProfitNOTE: This does not account for samples rejected by lab, but chargedfor and not generally recovered in the fee to the client. In that case thegross profit on a re-run would be $0.00.
17Veterinary Microbiology Culture System MultiChromeTMVeterinary Microbiology Culture SystemTo recap, MultiChrome™ offers the following:Speed: 24 hour to confirmation, identification & treatmentConvenience: In-house ability to test 9 areas affected by bacteriaReliability: Cutting edge technology with superior accuracyProfit Potential: A fraction of reference lab pricesProfessional Curiosity: “You were trained, you just need the tools!”MultiChrome GP(+)Exp 3/10Lot #123MultiChrome GP(-)MultiChrome™ “A different way to detect bacteria!”
18Veterinary Microbiology Culture System MultiChromeTMVeterinary Microbiology Culture SystemProduct details:MultiChrome™ is available in convenient packs of 5 or 10 plates:Five packs*Ten packs*Each plate individually packaged and sterilizedFull color reference insert included with each packShipped refrigerated, plates thaw in 5-10 min5-6 month expiration from date of shippingMultiChrome™ Micro Incubator*:Each incubator comes complete, ready to use with five trays*Contact your local distributor for exact pricing and for promotionsor discounts if applicable.MultiChrome GP(+)Exp 3/10Lot #123MultiChrome GP(-)