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Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Last Week:

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Presentation on theme: "Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Last Week:"— Presentation transcript:

1 Week 12w This Week: Unknown Project Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Last Week: Exp 43: Chemical Agents of Control: Chemotherapeutic Agents; Part A: Kirby Bauer Antibiotic Sensitivity Test

2 Exp 43: Chemical Agents of Control: Chemotherapeutic Agents Some chemotherapeutic agents have a limited spectrum of activity being effective against only one group of microorganisms. Other chemotherapeutic agents exhibit broad spectrum activity against a range of microorganisms. Drug susceptibilities on many pathogenic microorganisms are known, but it is sometimes necessary to test several agents to determine the drug of choice.

3 Exp. 69: Species Identification of Unknown Bacterial Cultures Unknown project overview. Requisition Forms. Written report. Part 2: –Gram Stain –Differential Media –Biochemical Tests

4 Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Disinfectants and antiseptics- chemicals used to prevent contamination and infection Efficacy is influenced by: –Concentration –Length of exposure –Type of microbial population to be destroyed –Environmental conditions

5 Exp 45: Chemical Agents of Control: Disinfectants and Antiseptics Part B: Agar Plate-Sensitivity Method Media: 2 TSA plates, 2 swabs Culture: EC & SA Procedure: (refer to pg. 307) Label plate w/org. Label plate with test solution. Heavily inoculate entire surface of plate. Saturate disks with test solution. Use forceps to fix disks to agar. - mouth wash - peroxide - iodine - hand soap (lab) Incubate plates at 37 o C for 24hrs in inverted position. Week 12W PER TABLE

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7 Epidemiology: the science of when and where diseases occur and how they are transmitted Descriptive (data on occurrence) Analytical (probable cause) Experimental (hypothesis, test) CDC, Center for Disease Control, US Public Health Service


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