1. INTRODUCTION ( ) Xi Zhang Shanghai Municipal Center for Disease Control

2. Guideline( ) Brief Introduction of SCDC SCDC Introduction of Lab of Microbiology Brief Introduction of Myself

3. Sight Seeing of SCDC (SCDC )

4. New Lab Buiding (SCDC )

5. Goals of SCDC ( ) Established Public System meet health needs meet health needs improve quality of citizen life improve quality of citizen life ensure the persistent social and economic development of the city. ensure the persistent social and economic development of the city.

6. Goals and Objectives of SCDC ( ) To expand responsibility on public health to meet social and individual needs To expand responsibility on public health to meet social and individual needs To improve the ability of rapid response to the urgent events and disasters; To offer high quality health services for community; To improve the preventive medicine research level To promote national and international collaboration

7. MISSION of SCDC ( ) Policy making and planning Surveillance and information synthesis Implementation of prevention and intervention Public health research Prevention and health care service

8. Dept of Chronic Diseases Prevention Dept of Health Care for Children&Adolescents Health Care for Children&Adolescents Dept of Health Care for Children&Adolescents Health Care for Children&Adolescents Dept of Nutrition Division for Chronic Diseases Prevention Dept of Epidemic Prevention Dept of Immunization Vaccine Management Dept of Vector Control & Prevention Dept of Parasitosis Control&Prevention Dept of Tuberculosis Control&Prevention Dept of HIV/AIDS,STD Control & Prevention Dept of Disinfection Lab of Microbiology Division for communicable Disease Control&Prevention Dept of Occupational Health Dept of Occupational Health Dept of Environmental Health Research Lab of Molecular Biology & Gene Division for Environmental & Occupational Health Dept of Environmental Health Evaluation Health Evaluation Dept of Environmental Health Evaluation Health Evaluation Dept of Toxicology Toxicology Lab of ChemicalAnalysis ChemicalAnalysis Dept of Food Health and Safety Lab of Experimental Animal Experimental Animal Lab of Experimental Animal Experimental Animal Division for Health & Safety Assessment Health & Safety Assessment Division for Health & Safety Assessment Health & Safety Assessment Office of Preventive Health Examination Office of Preventive Health Examination Office of Medical Examination for Drivers Dept of Occupational Health Examination Division for Health Care Service Health Care Service Division for Health Care Service Health Care Service Dept of Health Statistics Central library Dept of computer administration Division for Health Information Personnel & Security office Logistics office Financing (Auditing) office Assents Manage office Capital Construction office Quality control office Association Manage office Test Manage office Research and Education office Editorial Department Training Department Shanghai Municipal Center for Disease Control and Prevention Shanghai Institute of Preventive Medicine Shanghai Municipal Center for Disease Control and Prevention Shanghai Institute of Preventive Medicine Administration office SCDC Organization Chart

9. SCDC RESOURCE Employee: More than 700 persons Technical Employee: more than 530 persons Lab area: 32,000m 2 Instrument capital: over 200 million Yuan (RMB)

10. LAB MAMAGEMENT ORGANIZITION CHART

11. SCDC Lab Management Accredited with ISO /IEC17025:1999 certification Accredited with Metrology Certification Pay more attention to lab safety and bio-safety Employee technical training annual Developing LIMS

12. Tasks of Lab of Microbiology ( To monitor pathogens contaminating in environment (water, air), foods, air conditioners To monitor infectious diseases To do some research works for public health (especially in detection methods, food safety, professional health, environment health) To take part in dealing with public health emergency affairs To provide analysis data for surveillance and product safety. To participate in and issue quality control plan To provide professional and technical training for different microbiology lab

13. Structure of Lab of Microbiology ( Bacteria monitoring and testing section Respiratory bacteria group Enterobacterium group Vector borne bacteria group Serum antibody surveillance group Virus monitoring and testing section Respiratory virus group Enterovirus group Hepatitis virus group Vector borne virus group Serum antibody surveillance group

14. Structure of Lab of Microbiology ( Lab of Microbiology Bacteria sectionViruses section Respiratory bacteria group Enterobacterium group Surem antibody surveillance group Vector borne Bacteria group Respiratory virus group Enterovirus group Hepatitis virus group Arbonia virus group

15. Technical Platform of Lab of Microbiology Pathogens isolation and identification Immunoassay (ELISA, IFA, etal) Molecular biology assay (PCR, PFGE, Ribo- printer, DNA Sequence tests)

16. Pictures of Microbiology Lab Lab corridor A variety of microscope

17. More Pictures Nucleic acid extraction machine Real-time PCR instrument

18. More Pictures Temple for Bacteria counting ATB for Bacteria identification

19. Bio-safety level-3 Laboratory

20. Main co-operate Items Join in the Global Salmonella Surveillance (GSS) Join in the Pulse-Net Chine (which is co-operate with Pulse-Net USA) Join in the WHO Influenza surveillance and monitoring Net Join in the foods safety and inspects surveillance in China

21. Bacteria M onitoring & Testing Section Monitoring salmonella, shigella, Vibro Cholera, E.Coli O157:H7, Vibro Parahemolyticus, staphylococcus aureus, campylobacter Listeria Monocytogenes et al in foods (pork, beef, seafood, milk, vegetables et al), water supply and diarrhea patients Monitoring Legionalle bacillus in air conditioners, water distribution systems and patients Monitoring Neisseria meningitis, Group A Streptococcus and other respiratory bacteria in patient and public areasy Monitoring vector-borne pathegons Monitoring and testing pathogens in foodborne diseases and waterborne diseases Monitoring antibiotic sensitivity and antibiotic resistence Monitoring antibody level in population

22. Viruses Monitoring and Testing Section Monitoring HAV,HEV and Noro virus in seafood, water supply, riverhead and patients Monitoring influenza and other respiratory viruses in publics and patients Monitoring Avian influenza in publics and birds, poultries Monitoring Japanese Encephalitis virus, Dengue fever virus and Hanta virus, et al in mosquito, mouse and patients Monitoring Hepatitis virus such as HBV, HCV, HEV in patients Monitoring other diarrhea viruses in patients Monitoring hand-foot-mouth disease in children Monitoring virus-Antibody levels in population

23. Monitoring Results Comma bacillus positive in HuangPu River and sea foods Salmonella,pathogenic E. coli positive in pork and beef Parahaemorrhagic vibro positive in sea foods Legionella bacillus positive in water distribution systems and central air conditioner chilled water supplys HAV, Noro virus positive in sea foods Dengue virus, Japanese encephalitis virus negative in mosquito and Hanten virus antibody positive in mouse

24. The situation of Communicable disease in Shanghai,2007 Name of diseaes Number of patient Rate of incidence(1/10,000) Increase or decrease(%) Cholera10.01-50.34 AIDS580.4298.65 Hepatitis330924.09-29.23 Measles3972.8982.55 Rabies40.03-0.68 Japanese encephalitis 20.01- Dengue fever10.01-

25. The situation of Communicable disease (part) in Shanghai,2007 Name of diseasesNumber of patients Morbidity (1/10,000) Increase or decrease(%) bacillary dysentery 39010.12-52.75 TB429631.2813.72 Typhoid680.50-20.54 Epidemic cerebrospinal meningitis 50.0424.15 Brucellosis50.04- Gonorrhea605444.05-21.42 Syphilis874763.698.35

26. Personal Experience The Chief of Microbiology Lab Responsible for lab management, lab bio-safety, including quality and technical management Over 20 years lab practice Familiar with pathogens isolation, identification and molecular technique( such PCR and Real- time PCR) Graduated from the School of Public Health, Fudan University

27. Personal Research Field Microbiology pollution surveillance and monitor in foods, water supply and central air conditioner Pathogenic organisms detecting and results analysis in communicable disease patients Pathogenic organisms rapid testing methods research Search for unknown pathogens which causes communicated disease

28. Goals of Fogarty Training To understand the lab management here in California (lab safety, quality control) To learn the research strategies and methods To learn the planning & preparing of bioterrorism To learn the public health emergency response strategies To realize Californias culture, historical and social environment To improve my English

29. Thanks for Fogarty International Center of NIH California Department of Public Health, EHIB &EHLB Faculty members of Fogarty International Training Program All members in EHIB and EHLB

30. THANK YOU !