1. Intracellular EBV‐encoded LMP1 localizes to late endosomes.
Intracellular EBV‐encoded LMP1 localizes to late endosomes. CLSM analysis on cytospins of EBV‐infected LCLs showing the subcellular localization of endogenous LMP1 in combination with subcellular markers (A). Shown are representative images of individual LCL cells with a polarized phenotype and ‘intermediate’ levels of LMP1 co‐stained with GM130 (cis‐Golgi; 34%), TGN46 (trans‐Golgi; 30%), EEA1 (early endosomes; 24%), CD63/Lamp3 (late endosomes; 92%) or LAMP1 (lysosomes; 13%). Percentages represent the proportion of cells with co‐localization as shown in Supplementary Figure S1. (B) Pearson correlation coefficients (PCCs) were calculated from multiple individual cells expressing ‘intermediate’ levels LMP1 and the indicated subcellular markers (error bars represent s.d.; n>5). (C) The percentage co‐localization calculated in (B) were multiplied by the proportion of cells (n>50) that display co‐localization in overview images (Supplementary Figure S1) (GM130 represents Golgi=1).
Frederik J Verweij et al. EMBO J. 2011;30:
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