1. From: Elevated Expression of O-GlcNAc–Modified Proteins and O-GlcNAc Transferase in Corneas of Diabetic Goto-Kakizaki Rats
Invest. Ophthalmol. Vis. Sci ;44(9): doi: /iovs
Figure Legend:
(A) Distribution of immunofluorescence with anti-O-GlcNAc in the Wistar rat corneal epithelium cultured in the absence (a, c) or presence (b, d) of 100 μM PUGNAc. Corneas were fixed with 4% paraformaldehyde. Frozen sections (10 μm in thickness) were reacted with anti-O-GlcNAc antibody (RL2) and then with fluorescence-conjugated goat anti-mouse IgG. Nuclei were stained with nucleic acid stain and the sections were observed with a laser scanning confocal microscope. (a, b) Green indicates the distribution of anti-O-GlcNAc reactivity. (c, d) Nucleic acid staining images (blue), which are the same optical field as in a and b, respectively. Ep, corneal epithelium; S, stroma. Scale bar, 10 μm. (B) Quantification of anti-O-GlcNAc immunoreactivity. The intensity of anti-O-GlcNAc immunoreactivity in both the nucleus and cytoplasm of the corneal epithelial cells cultured in the presence of PUGNAc increased significantly compared with that in the corneal epithelium cultured in the absence of PUGNAc. Data represent the mean ± SEM (n = 6). *P <
Date of download: 10/24/2017
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